Restriction map for pF1KE0608
##################################
# NO CUT ENZYMES (  138 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AcyI        AflII       AgeI        AhdI        Alw44I      
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AviII       AvrII       BalI        
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BfrI        BglI        BlnI        BpuAI       
BsaAI       BsaBI       BsaHI       BsgI        BsiEI       
BsiWI       BsmBI       Bsp1407I    BspHI       BspLU11I    
BsrBI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstZ17I     Bsu36I      CpoI        DraIII      DsaI        
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoO65I     EcoRI       EcoRV       
EcoT22I     FauI        FseI        FspI        HaeII       
HgaI        Hin1I       HpaI        KasI        KpnI        
KspI        MamI        MfeI        MluI        MluNI       
MscI        MunI        MvnI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacII       SalI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
Tth111I     Van91I      XbaI        XcmI        XhoI        
XmaCI       XmaI        XmnI        


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# ONE CUT ENZYMES (   50 enzymes) #
###################################

AccIII        460  TCCGGA               T^CCGGA
AflIII        787  ACGTGT               A^C[GA][CT]GT
AvaI          158  CTCGGG               C^[CT]CG[GA]G
BamHI         931  GGATCC               G^GATCC
BanI          814  GGTGCC               G^G[CT][GA]CC
BanII         750  GAGCTC               G[GA]GC[CT]^C
BclI          449  TGATCA               T^GATCA
BcnI          986  CCCGG                CC^[GC]GG
BfaI          133  CTAG                 C^TAG
BglII         577  AGATCT               A^GATCT
BlpI          747  GCTGAGC              GC^T[ACGT]AGC
BpmI          804  CTCCAG               CTGGAG(16/14)
Bpu1102I      747  GCTGAGC              GC^T[ACGT]AGC
BsaI          215  GGTCTC               GGTCTC(1/5)
BseAI         460  TCCGGA               T^CCGGA
BsmI          300  GAATGC               GAATGC(1/-1)
BsoBI         158  CTCGGG               C^[CT]CG[GA]G
BspDI         713  ATCGAT               AT^CGAT
BspEI         460  TCCGGA               T^CCGGA
BsrDI          32  GCAATG               GCAATG(2/0)
BsrFI          91  ACCGGC               [GA]^CCGG[CT]
BstXI         755  CCATGACGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII         747  GCTGAGC              GC^T[ACGT]AGC
CfoI           56  GCGC                 GCG^C
Cfr10I         91  ACCGGC               [GA]^CCGG[CT]
ClaI          713  ATCGAT               AT^CGAT
DraI          540  TTTAAA               TTT^AAA
DrdI          786  GACGTGTGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI         682  CCTGGGCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I       860  CCAAGG               C^C[AT][AT]GG
FbaI          449  TGATCA               T^GATCA
HhaI           56  GCGC                 GCG^C
HinP1I         56  GCGC                 G^CGC
HincII        442  GTTGAC               GT[CT]^[GA]AC
HindII        442  GTTGAC               GT[CT]^[GA]AC
HindIII       226  AAGCTT               A^AGCTT
MaeI          133  CTAG                 C^TAG
MaeII         787  ACGT                 A^CGT
MroI          460  TCCGGA               T^CCGGA
NciI          986  CCCGG                CC^[GC]GG
NheI          132  GCTAGC               G^CTAGC
NspI          968  GCATGT               [GA]CATG^[CT]
PpuMI         976  GGGACCC              [GA]G^G[AT]CC[CT]
PvuII         732  CAGCTG               CAG^CTG
SacI          750  GAGCTC               GAGCT^C
SapI          990  GAAGAGC              GCTCTTC(1/4)
StyI          860  CCAAGG               C^C[AT][AT]GG
TaqI          714  TCGA                 T^CGA
Tsp45I        203  GTGAC                ^GT[GC]AC
TthHB8I       714  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AciI           46  GCGG                 CCGC(-3/-1)
              929  GCGG                
AcsI          272  AAATTC               [GA]^AATT[CT]
             1027  AAATTT              
AfaI          573  GTAC                 GT^AC
              836  GTAC                
AlwI          932  GATCC                GGATC(4/5)
              931  GGATC               
ApoI          272  AAATTC               [GA]^AATT[CT]
             1027  AAATTT              
AspHI         411  GAGCAC               G[AT]GC[AT]^C
              750  GAGCTC              
BmyI          411  GAGCAC               G[AGT]GC[ACT]^C
              750  GAGCTC              
BseRI         309  CTCCTC               GAGGAG(10/8)
              882  GAGGAG              
BsiHKAI       411  GAGCAC               G[AT]GC[AT]^C
              750  GAGCTC              
BsmFI         162  GGGAC                GGGAC(10/14)
              976  GGGAC               
Bsp1286I      411  GAGCAC               G[AGT]GC[ACT]^C
              750  GAGCTC              
BspMI         467  ACCTGC               ACCTGC(4/8)
              534  GCAGGT              
BsrI          632  CCAGT                ACTGG(1/-1)
              722  CCAGT               
BstF5I        654  GGATG                GGATG(2/0)
              870  GGATG               
BstYI         577  AGATCT               [GA]^GATC[CT]
              931  GGATCC              
DraII         286  AGGCCCC              [GA]G^G[ACGT]CC[CT]
              976  GGGACCC             
EarI          990  GAAGAG               CTCTTC(1/4)
             1057  GAAGAG              
EcoO109I      286  AGGCCCC              [GA]G^G[ACGT]CC[CT]
              976  GGGACCC             
FokI          654  GGATG                GGATG(9/13)
              870  GGATG               
Ksp632I       990  GAAGAG               CTCTTC(1/4)
             1057  GAAGAG              
MflI          577  AGATCT               [GA]^GATC[CT]
              931  GGATCC              
MslI          610  CATGGTGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              756  CATGACGATG          
MspA1I        732  CAGCTG               C[AC]G^C[GT]G
              927  CAGCGG              
PleI          483  GACTC                GAGTC(4/5)
              802  GACTC               
PstI          532  CTGCAG               CTGCA^G
              583  CTGCAG              
RsaI          573  GTAC                 GT^AC
              836  GTAC                
SfaNI         616  GATGC                GCATC(5/9)
              911  GCATC               
SfcI          532  CTGCAG               C^T[GA][CT]AG
              583  CTGCAG              
XhoII         577  AGATCT               [GA]^GATC[CT]
              931  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   41 enzymes) #
#########################################

AluI        AvaII       BbvI        BsaJI       BsaWI       
BsiYI       BslI        BsmAI       BssKI       BstNI       
Cac8I       Cfr13I      DdeI        DpnII       EcoRII      
Fnu4HI      HaeIII      HapII       HinfI       HpaII       
HphI        ItaI        MaeIII      MboI        MboII       
MnlI        MseI        MspI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       Sau3AI      Sau96I      
ScrFI       TfiI        Tru9I       TseI        Tsp509I     
TspRI       
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