Restriction map for pF1KE0608
 (Linear) (Six-base) MAPSORT of: KIEE0608.seq  Check: 115  from: 1  to: 1077



KIEE0608.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:16  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     804    1077 
   Size:        804     273
 
AflIII A'CryG_T

Cuts at:      0     787    1077 
   Size:        787     290
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     642    1077 
   Size:        642     435
 
ApoI r'AATT_y

Cuts at:      0     272    1027    1077 
   Size:        272     755      50

  Fragments arranged by size: 

                 755     272      50
 
AvaI C'yCGr_G

Cuts at:      0     158    1077 
   Size:        158     919
 
BamHI G'GATC_C

Cuts at:      0     931    1077 
   Size:        931     146
 
BanI G'GyrC_C

Cuts at:      0     814    1077 
   Size:        814     263
 
BanII G_rGCy'C

Cuts at:      0     754    1077 
   Size:        754     323
 
BclI T'GATC_A

Cuts at:      0     449    1077 
   Size:        449     628
 
BglII A'GATC_T

Cuts at:      0     577    1077 
   Size:        577     500
 
BlpI GC'TnA_GC

Cuts at:      0     748    1077 
   Size:        748     329
 
BmgBI CAC'GTC

Cuts at:      0     788    1077 
   Size:        788     289
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     626    1077 
   Size:        626     451
 
BmtI G_CTAG'C

Cuts at:      0     136    1077 
   Size:        136     941
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     789    1077 
   Size:        789     288
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     221    1077 
   Size:        221     856
 
BsaWI w'CCGG_w

Cuts at:      0     460     707     905    1077 
   Size:        460     247     198     172

  Fragments arranged by size: 

                 460     247     198     172
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      29     900     930    1077 
   Size:         29     871      30     147

  Fragments arranged by size: 

                 871     147      30      29
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     300     897    1077 
   Size:        300     597     180

  Fragments arranged by size: 

                 597     300     180
 
BseYI C'CCAG_C

Cuts at:      0      58     354     693    1077 
   Size:         58     296     339     384

  Fragments arranged by size: 

                 384     339     296      58
 
BsiHKAI G_wGCw'C

Cuts at:      0     415     754    1077 
   Size:        415     339     323

  Fragments arranged by size: 

                 415     339     323
 
BsmI GAATG_Cn'

Cuts at:      0     306    1077 
   Size:        306     771
 
Bsp1286I G_dGCh'C

Cuts at:      0     415     754    1077 
   Size:        415     339     323

  Fragments arranged by size: 

                 415     339     323
 
BspEI T'CCGG_A

Cuts at:      0     460    1077 
   Size:        460     617
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     476     525    1077 
   Size:        476      49     552

  Fragments arranged by size: 

                 552     476      49
 
BsrDI GCAATG_nn'

Cuts at:      0      39    1077 
   Size:         39    1038
 
BsrFI r'CCGG_y

Cuts at:      0      91    1077 
   Size:         91     986
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     762    1077 
   Size:        762     315
 
BstYI r'GATC_y

Cuts at:      0     577     931    1077 
   Size:        577     354     146

  Fragments arranged by size: 

                 577     354     146
 
ClaI AT'CG_AT

Cuts at:      0     714    1077 
   Size:        714     363
 
DraI TTT'AAA

Cuts at:      0     542    1077 
   Size:        542     535
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     792    1077 
   Size:        792     285
 
EarI CTCTTCn'nnn_

Cuts at:      0     985    1052    1077 
   Size:        985      67      25

  Fragments arranged by size: 

                 985      67      25
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      61    1077 
   Size:         61    1016
 
EcoICRI GAG'CTC

Cuts at:      0     752    1077 
   Size:        752     325
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     789     804    1077 
   Size:        789      15     273

  Fragments arranged by size: 

                 789     273      15
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     686    1077 
   Size:        686     391
 
EcoO109I rG'GnC_Cy

Cuts at:      0     287     977    1077 
   Size:        287     690     100

  Fragments arranged by size: 

                 690     287     100
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1038    1077 
   Size:       1038      39
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     468     500     562     594     646     678    1077 
   Size:        468      32      62      32      52      32     399

  Fragments arranged by size: 

                 468     399      62      52      32      32      32
 
HincII GTy'rAC

Cuts at:      0     444    1077 
   Size:        444     633
 
HindIII A'AGCT_T

Cuts at:      0     226    1077 
   Size:        226     851
 
MslI CAynn'nnrTG

Cuts at:      0     614     760    1077 
   Size:        614     146     317

  Fragments arranged by size: 

                 614     317     146
 
MspA1I CmG'CkG

Cuts at:      0     734     929    1077 
   Size:        734     195     148

  Fragments arranged by size: 

                 734     195     148
 
NheI G'CTAG_C

Cuts at:      0     132    1077 
   Size:        132     945
 
NspI r_CATG'y

Cuts at:      0     972    1077 
   Size:        972     105
 
PpuMI rG'GwC_Cy

Cuts at:      0     977    1077 
   Size:        977     100
 
PstI C_TGCA'G

Cuts at:      0     536     587    1077 
   Size:        536      51     490

  Fragments arranged by size: 

                 536     490      51
 
PvuII CAG'CTG

Cuts at:      0     734    1077 
   Size:        734     343
 
SacI G_AGCT'C

Cuts at:      0     754    1077 
   Size:        754     323
 
SanDI GG'GwC_CC

Cuts at:      0     977    1077 
   Size:        977     100
 
SapI GCTCTTCn'nnn_

Cuts at:      0     985    1077 
   Size:        985      92
 
SfcI C'TryA_G

Cuts at:      0     532     583    1077 
   Size:        532      51     494

  Fragments arranged by size: 

                 532     494      51
 
StyI C'CwwG_G

Cuts at:      0     860    1077 
   Size:        860     217
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     555    1077 
   Size:        555     522
 
TatI w'GTAC_w

Cuts at:      0     572     835    1077 
   Size:        572     263     242

  Fragments arranged by size: 

                 572     263     242
 

 Enzymes that do cut:

     AcuI   AflIII     AlfI     ApoI     AvaI    BamHI     BanI    BanII
     BclI    BglII     BlpI    BmgBI     BmrI     BmtI     BpmI     BsaI
    BsaWI    BsaXI    BseRI    BseYI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspMI    BsrDI    BsrFI    BstXI    BstYI     ClaI     DraI     DrdI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI    Hin4I
   HincII  HindIII     MslI   MspA1I     NheI     NspI    PpuMI     PstI
    PvuII     SacI    SanDI     SapI     SfcI     StyI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI     BbeI     BbsI    BbvCI     BcgI    BciVI
    BfrBI     BglI Bme1580I     BplI   Bpu10I    BpuEI    BsaAI    BsaBI
    BsaHI     BsgI    BsiEI    BsiWI    BsmBI    BspHI    BsrBI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI    CspCI   DraIII     EaeI     EagI    EcoRI    EcoRV
     FseI     FspI    FspAI    HaeII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    RsrII    SacII     SalI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI
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