Restriction map for pF1KE0595
##################################
# NO CUT ENZYMES (  151 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AcsI        AflII       AflIII      AgeI        Alw44I      
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspHI       AspI        AviII       AvrII       
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BclI        BfaI        BfrI        BglI        BlnI        
BlpI        BpmI        Bpu1102I    BpuAI       BsaAI       
BsaI        BsgI        BsiEI       BsiHKAI     BsiWI       
BsmI        Bsp1407I    BspDI       BspHI       BspLU11I    
BspMI       BsrGI       BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstUI       BstXI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
DdeI        DraI        DraII       DraIII      DrdI        
DsaI        EagI        EclXI       Eco47III    Eco52I      
Eco81I      EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT14I     EcoT22I     FbaI        FseI        FspI        
HgaI        HincII      HindII      HindIII     HpaI        
KpnI        KspI        MaeI        MluI        MseI        
MslI        MspA1I      MvnI        NaeI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SbfI        ScaI        
SexAI       SfcI        SfiI        SfuI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        Tru9I       
TspRI       Tth111I     Van91I      XbaI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AccIII        174  TCCGGA               T^CCGGA
AcyI          465  GGCGCC               G[GA]^CG[CT]C
AfaI          126  GTAC                 GT^AC
AhdI           78  GACCCCGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI          78  GACCCCGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI          380  TGGCCA               TGG^CCA
BamHI         307  GGATCC               G^GATCC
BanII         178  GAGCCC               G[GA]GC[CT]^C
BbeI          465  GGCGCC               GGCGC^C
BglII         416  AGATCT               A^GATCT
BmyI          178  GAGCCC               G[AGT]GC[ACT]^C
BsaBI         411  GATTCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         465  GGCGCC               G[GA]^CG[CT]C
BseAI         174  TCCGGA               T^CCGGA
BseRI         325  CTCCTC               GAGGAG(10/8)
Bsp1286I      178  GAGCCC               G[AGT]GC[ACT]^C
BspEI         174  TCCGGA               T^CCGGA
BsrBI         115  GAGCGG               CCGCTC(-3/-3)
BsrFI         233  ACCGGC               [GA]^CCGG[CT]
BsrI          219  CCAGT                ACTGG(1/-1)
BstAPI        482  GCATCCGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I        483  CATCC                GGATG(2/0)
CfoI          466  GCGC                 GCG^C
Cfr10I        233  ACCGGC               [GA]^CCGG[CT]
EaeI          380  TGGCCA               [CT]^GGCC[GA]
Eam1105I       78  GACCCCGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI          30  CCTCCCCGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI          270  CCCGC                CCCGC(4/6)
FokI          483  CATCC                GGATG(9/13)
HaeII         465  GGCGCC               [GA]GCGC^[CT]
HhaI          466  GCGC                 GCG^C
Hin1I         465  GGCGCC               G[GA]^CG[CT]C
HinP1I        466  GCGC                 G^CGC
HphI          302  TCACC                GGTGA(8/7)
KasI          465  GGCGCC               G^GCGCC
MaeII         264  ACGT                 A^CGT
MaeIII        266  GTGAC                ^GT[ACGT]AC
MamI          411  GATTCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI          503  CAATTG               C^AATTG
MluNI         380  TGGCCA               TGG^CCA
MroI          174  TCCGGA               T^CCGGA
MscI          380  TGGCCA               TGG^CCA
MunI          503  CAATTG               C^AATTG
NarI          465  GGCGCC               GG^CGCC
NlaIII        215  CATG                 CATG^
RsaI          126  GTAC                 GT^AC
SfoI          465  GGCGCC               GGC^GCC
SgrAI         232  CACCGGCG             C[GA]^CCGG[CT]G
Tsp45I        266  GTGAC                ^GT[GC]AC
Tsp509I       504  AATT                 ^AATT


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AvaII          49  GGACC                G^G[AT]CC
              186  GGTCC               
BanI          443  GGCACC               G^G[CT][GA]CC
              465  GGCGCC              
BsmAI         261  GAGAC                GTCTC(1/5)
              388  GAGAC               
BsmBI         261  GAGACG               CGTCTC(1/5)
              388  GAGACG              
BsmFI          48  GGGAC                GGGAC(10/14)
              450  GGGAC               
BstYI         307  GGATCC               [GA]^GATC[CT]
              416  AGATCT              
MflI          307  GGATCC               [GA]^GATC[CT]
              416  AGATCT              
PleI           84  GAGTC                GAGTC(4/5)
              403  GAGTC               
SmaI          129  CCCGGG               CCC^GGG
              256  CCCGGG              
TfiI          411  GATTC                G^A[AT]TC
              429  GAATC               
XcmI          143  CCAAAGAAGAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              368  CCAGGGAAGAGCTGG     
XhoII         307  GGATCC               [GA]^GATC[CT]
              416  AGATCT              
XmaCI         129  CCCGGG               C^CCGGG
              256  CCCGGG              
XmaI          129  CCCGGG               C^CCGGG
              256  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   43 enzymes) #
#########################################

AciI        AluI        AlwI        AvaI        BbvI        
BcnI        BsaJI       BsaWI       BsiYI       BslI        
BsoBI       BsrDI       BssKI       BstNI       Cac8I       
Cfr13I      DpnII       EarI        EcoRII      Fnu4HI      
HaeIII      HapII       HinfI       HpaII       ItaI        
Ksp632I     MboI        MboII       MnlI        MspI        
MvaI        MwoI        NciI        NdeII       NlaIV       
SapI        Sau3AI      Sau96I      ScrFI       SfaNI       
TaqI        TseI        TthHB8I     
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