Restriction map for pF1KE0594
##################################
# NO CUT ENZYMES (  146 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcsI        
AflII       AflIII      AgeI        Alw44I      AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspHI       
AspI        AviII       AvrII       BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaI        BsgI        BsiEI       BsiHKAI     BsiWI       
Bsp1407I    BspDI       BspHI       BspLU11I    BspMI       
BsrGI       BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstXI       BstZ17I     Bsu36I      
CelII       ClaI        CpoI        DdeI        DraI        
DraII       DraIII      DrdI        DsaI        EagI        
EclXI       Eco47III    Eco52I      Eco81I      EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT14I     EcoT22I     
FbaI        FseI        FspI        HincII      HindII      
HindIII     HpaI        KpnI        KspI        MaeI        
MaeII       MaeIII      MamI        MluI        MseI        
MslI        NaeI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        RcaI        
RsrII       SacI        SacII       SalI        SbfI        
ScaI        SexAI       SfcI        SfiI        SfuI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
Tru9I       Tsp45I      Tth111I     XbaI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   58 enzymes) #
###################################

AccII         265  CGCG                 CG^CG
AccIII        174  TCCGGA               T^CCGGA
AcyI          465  GGCGCC               G[GA]^CG[CT]C
AfaI          126  GTAC                 GT^AC
AhdI           78  GACCCCGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI         441  CAGGCACTG            CAG[ACGT][ACGT][ACGT]^CTG
AspEI          78  GACCCCGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI          380  TGGCCA               TGG^CCA
BamHI         307  GGATCC               G^GATCC
BanI          465  GGCGCC               G^G[CT][GA]CC
BanII         178  GAGCCC               G[GA]GC[CT]^C
BbeI          465  GGCGCC               GGCGC^C
BmyI          178  GAGCCC               G[AGT]GC[ACT]^C
BsaHI         465  GGCGCC               G[GA]^CG[CT]C
BseAI         174  TCCGGA               T^CCGGA
BseRI         325  CTCCTC               GAGGAG(10/8)
BsmI          359  GCATTC               GAATGC(1/-1)
Bsp1286I      178  GAGCCC               G[AGT]GC[ACT]^C
BspEI         174  TCCGGA               T^CCGGA
BsrBI         115  GAGCGG               CCGCTC(-3/-3)
BsrFI         233  ACCGGC               [GA]^CCGG[CT]
BstAPI        482  GCATCCGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I        483  CATCC                GGATG(2/0)
BstUI         265  CGCG                 CG^CG
BstYI         307  GGATCC               [GA]^GATC[CT]
CfoI          466  GCGC                 GCG^C
Cfr10I        233  ACCGGC               [GA]^CCGG[CT]
EaeI          380  TGGCCA               [CT]^GGCC[GA]
Eam1105I       78  GACCCCGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI          30  CCTCCCCGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI          270  CCCGC                CCCGC(4/6)
FokI          483  CATCC                GGATG(9/13)
HaeII         465  GGCGCC               [GA]GCGC^[CT]
HgaI          263  GACGC                GACGC(5/10)
HhaI          466  GCGC                 GCG^C
Hin1I         465  GGCGCC               G[GA]^CG[CT]C
HinP1I        466  GCGC                 G^CGC
HphI          302  TCACC                GGTGA(8/7)
KasI          465  GGCGCC               G^GCGCC
MfeI          503  CAATTG               C^AATTG
MflI          307  GGATCC               [GA]^GATC[CT]
MluNI         380  TGGCCA               TGG^CCA
MroI          174  TCCGGA               T^CCGGA
MscI          380  TGGCCA               TGG^CCA
MspA1I        151  CAGCTG               C[AC]G^C[GT]G
MunI          503  CAATTG               C^AATTG
MvnI          265  CGCG                 CG^CG
NarI          465  GGCGCC               GG^CGCC
NlaIII        215  CATG                 CATG^
PflMI         440  CCAGGCACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII         151  CAGCTG               CAG^CTG
RsaI          126  GTAC                 GT^AC
SfoI          465  GGCGCC               GGC^GCC
SgrAI         232  CACCGGCG             C[GA]^CCGG[CT]G
Tsp509I       504  AATT                 ^AATT
TspRI         445  CACTG                CA[GC]TG[ACGT][ACGT]^
Van91I        440  CCAGGCACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         307  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AvaII          49  GGACC                G^G[AT]CC
              186  GGTCC               
BbvI          228  GCAGC                GCAGC(8/12)
              496  GCAGC               
BsmAI         261  GAGAC                GTCTC(1/5)
              388  GAGAC               
BsmBI         261  GAGACG               CGTCTC(1/5)
              388  GAGACG              
BsmFI          48  GGGAC                GGGAC(10/14)
              450  GGGAC               
BsrDI         135  CATTGC               GCAATG(2/0)
              491  GCAATG              
BsrI          446  ACTGG                ACTGG(1/-1)
              219  CCAGT               
EarI          373  GAAGAG               CTCTTC(1/4)
              434  GAAGAG              
Ksp632I       373  GAAGAG               CTCTTC(1/4)
              434  GAAGAG              
MboII         373  GAAGA                GAAGA(8/7)
              434  GAAGA               
PleI           84  GAGTC                GAGTC(4/5)
              403  GAGTC               
SapI          373  GAAGAGC              GCTCTTC(1/4)
              434  GAAGAGC             
SmaI          129  CCCGGG               CCC^GGG
              256  CCCGGG              
TfiI          411  GATTC                G^A[AT]TC
              429  GAATC               
TseI          228  GCAGC                G^C[AT]GC
              496  GCAGC               
XcmI          143  CCAAAGAACAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              368  CCAGGGAAGAGCTGG     
XmaCI         129  CCCGGG               C^CCGGG
              256  CCCGGG              
XmaI          129  CCCGGG               C^CCGGG
              256  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   36 enzymes) #
#########################################

AciI        AluI        AlwI        AvaI        BcnI        
BsaJI       BsaWI       BsiYI       BslI        BsoBI       
BssKI       BstNI       Cac8I       Cfr13I      DpnII       
EcoRII      Fnu4HI      HaeIII      HapII       HinfI       
HpaII       ItaI        MboI        MnlI        MspI        
MvaI        MwoI        NciI        NdeII       NlaIV       
Sau3AI      Sau96I      ScrFI       SfaNI       TaqI        
TthHB8I     
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