Restriction map for pF1KE0594
 (Circular) (Six-base) MAPSORT of: pF1KE0594.seq  Check: 3969  from: 1  to: 3623



pF1KE0594  3623 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:16  ..

AccI GT'mk_AC

Cuts at:    661     661 
   Size:       3623
 
Acc65I G'GTAC_C

Cuts at:    639     639 
   Size:       3623
 
AclI AA'CG_TT

Cuts at:   3463    3463 
   Size:       3623
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1448    1880    2627    2895    1448 
   Size:        432     747     268    2176

  Fragments arranged by size: 

                2176     747     432     268
 
AflIII A'CryG_T

Cuts at:    975    2079    3056     975 
   Size:       1104     977    1542

  Fragments arranged by size: 

                1542    1104     977
 
AgeI A'CCGG_T

Cuts at:   2011    2011 
   Size:       3623
 
AhdI GACnn_n'nnGTC

Cuts at:    191     191 
   Size:       3623
 
AlwNI CAG_nnn'CTG

Cuts at:    554     928    2495     554 
   Size:        374    1567    1682

  Fragments arranged by size: 

                1682    1567     374
 
ApaLI G'TGCA_C

Cuts at:   2393    2393 
   Size:       3623
 
ApoI r'AATT_y

Cuts at:    627    2976    3232     627 
   Size:       2349     256    1018

  Fragments arranged by size: 

                2349    1018     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3623
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3623
 
AvaI C'yCGr_G

Cuts at:    142     189     237     364     643     142 
   Size:         47      48     127     279    3122

  Fragments arranged by size: 

                3122     279     127      48      47
 
BamHI G'GATC_C

Cuts at:    415     648     415 
   Size:        233    3390
 
BanI G'GyrC_C

Cuts at:    573     639    1053    1301    1336     573 
   Size:         66     414     248      35    2860

  Fragments arranged by size: 

                2860     414     248      66      35
 
BanII G_rGCy'C

Cuts at:    290     637    1667     290 
   Size:        347    1030    2246

  Fragments arranged by size: 

                2246    1030     347
 
BbeI G_GCGC'C

Cuts at:    577    1057    1305     577 
   Size:        480     248    2895

  Fragments arranged by size: 

                2895     480     248
 
BciVI GTATCCnnnnn_n'

Cuts at:   1521    2282    3623    1521 
   Size:        761    1341    1521

  Fragments arranged by size: 

                1521    1341     761
 
BclI T'GATC_A

Cuts at:   1143    1143 
   Size:       3623
 
BglII A'GATC_T

Cuts at:   1138    3175    1138 
   Size:       2037    1586
 
BlpI GC'TnA_GC

Cuts at:    735     735 
   Size:       3623
 
Bme1580I G_kGCm'C

Cuts at:   1248    1341    2397    1248 
   Size:         93    1056    2474

  Fragments arranged by size: 

                2474    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    564     835     916    1251    3395     564 
   Size:        271      81     335    2144     792

  Fragments arranged by size: 

                2144     792     335     271      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1990    1990 
   Size:       3623
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    799    1678    2170    2468     799 
   Size:        879     492     298    1954

  Fragments arranged by size: 

                1954     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3327    3327 
   Size:       3623
 
BsaAI yAC'GTr

Cuts at:    976     976 
   Size:       3623
 
BsaBI GATnn'nnATC

Cuts at:      6    1137    3174       6 
   Size:       1131    2037     455

  Fragments arranged by size: 

                2037    1131     455
 
BsaHI Gr'CG_yC

Cuts at:    574    1054    1302    2004    3500     574 
   Size:        480     248     702    1496     697

  Fragments arranged by size: 

                1496     702     697     480     248
 
BsaWI w'CCGG_w

Cuts at:    282     412     593     812    1333    2011    2285    2432    2843 
   Size:        130     181     219     521     678     274     147     411

Cuts at:   2843     282 
   Size:       1062

  Fragments arranged by size: 

                1062     678     521     411     274     219     181     147
                 130
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    424     424 
   Size:       3623
 
BseYI C'CCAG_C

Cuts at:   1048    1806    2383    2879    1048 
   Size:        758     577     496    1792

  Fragments arranged by size: 

                1792     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1165    1211    2419     104 
   Size:       1061      46    1208    1308

  Fragments arranged by size: 

                1308    1208    1061      46
 
BsiHKAI G_wGCw'C

Cuts at:    637    1415    1605    2397     637 
   Size:        778     190     792    1863

  Fragments arranged by size: 

                1863     792     778     190
 
BsmI GAATG_Cn'

Cuts at:    467     467 
   Size:       3623
 
BsmBI CGTCTCn'nnnn_

Cuts at:    363     490     363 
   Size:        127    3496
 
Bsp1286I G_dGCh'C

Cuts at:    290     637    1248    1341    1415    1605    1667    2397     290 
   Size:        347     611      93      74     190      62     730    1516

  Fragments arranged by size: 

                1516     730     611     347     190      93      74      62
            
 
BspEI T'CCGG_A

Cuts at:    282    2843     282 
   Size:       2561    1062
 
BspHI T'CATG_A

Cuts at:   2799    2799 
   Size:       3623
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    673     887    1189    1570     673 
   Size:        214     302     381    2726

  Fragments arranged by size: 

                2726     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     225    1915    1969    3453    3618      50 
   Size:        175    1690      54    1484     165      55

  Fragments arranged by size: 

                1690    1484     175     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    242     606    1535     242 
   Size:        364     929    2330

  Fragments arranged by size: 

                2330     929     364
 
BsrFI r'CCGG_y

Cuts at:    341    1621    1802    2011     341 
   Size:       1280     181     209    1953

  Fragments arranged by size: 

                1953    1280     209     181
 
BssSI C'ACGA_G

Cuts at:   1894    2252    1894 
   Size:        358    3265
 
BstAPI GCAn_nnn'nTGC

Cuts at:    596    1000     596 
   Size:        404    3219
 
BstBI TT'CG_AA

Cuts at:   1984    1984 
   Size:       3623
 
BstYI r'GATC_y

Cuts at:      1     415     648    1115    1138    1473    1719    2731    2817 
   Size:        414     233     467      23     335     246    1012      86

Cuts at:   2817    2829    3175       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1012     467     449     414     346     335     246     233
                  86      23      12
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       3623
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    215    1573    1754    1917     215 
   Size:       1358     181     163    1921

  Fragments arranged by size: 

                1921    1358     181     163
 
BtsI GCAGTG_nn'

Cuts at:    866     866 
   Size:       3623
 
DraI TTT'AAA

Cuts at:    622     622 
   Size:       3623
 
DrdI GACnn_nn'nnGTC

Cuts at:   1329    2187    1329 
   Size:        858    2765
 
EaeI y'GGCC_r

Cuts at:    488    1208    1382    1773    1800    3145     488 
   Size:        720     174     391      27    1345     966

  Fragments arranged by size: 

                1345     966     720     391     174      27
 
EagI C'GGCC_G

Cuts at:   1208    1208 
   Size:       3623
 
EarI CTCTTCn'nnn_

Cuts at:    476     537    1646    1856     476 
   Size:         61    1109     210    2243

  Fragments arranged by size: 

                2243    1109     210      61
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1831    2141    2287    3433    1831 
   Size:        310     146    1146    2021

  Fragments arranged by size: 

                2021    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    635     635 
   Size:       3623
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1448    1880    2627    2895    1448 
   Size:        432     747     268    2176

  Fragments arranged by size: 

                2176     747     432     268
 
EcoNI CCTnn'n_nnAGG

Cuts at:    142     142 
   Size:       3623
 
EcoO109I rG'GnC_Cy

Cuts at:    762     762 
   Size:       3623
 
EcoRI G'AATT_C

Cuts at:    627     627 
   Size:       3623
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    474    1642     474 
   Size:       1168    2455
 
FspI TGC'GCA

Cuts at:   1404    1404 
   Size:       3623
 
HaeII r_GCGC'y

Cuts at:    577    1057    1305    2327    3306     577 
   Size:        480     248    1022     979     894

  Fragments arranged by size: 

                1022     979     894     480     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    177     209    1465    1495    1497    1527     177 
   Size:         32    1256      30       2      30    2273

  Fragments arranged by size: 

                2273    1256      32      30      30       2
 
HincII GTy'rAC

Cuts at:    662     662 
   Size:       3623
 
KasI G'GCGC_C

Cuts at:    573    1053    1301     573 
   Size:        480     248    2895

  Fragments arranged by size: 

                2895     480     248
 
KpnI G_GTAC'C

Cuts at:    643     643 
   Size:       3623
 
MfeI C'AATT_G

Cuts at:    611     611 
   Size:       3623
 
MluI A'CGCG_T

Cuts at:   3056    3056 
   Size:       3623
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2294    2478    2294 
   Size:        184    3439
 
MscI TGG'CCA

Cuts at:    490    1384     490 
   Size:        894    2729
 
MslI CAynn'nnrTG

Cuts at:   1612    1739    1612 
   Size:        127    3496
 
MspA1I CmG'CkG

Cuts at:    261     734    1048    1408    2421    2666     261 
   Size:        473     314     360    1013     245    1218

  Fragments arranged by size: 

                1218    1013     473     360     314     245
 
NaeI GCC'GGC

Cuts at:   1804    1804 
   Size:       3623
 
NarI GG'CG_CC

Cuts at:    574    1054    1302     574 
   Size:        480     248    2895

  Fragments arranged by size: 

                2895     480     248
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       3623
 
NgoMIV G'CCGG_C

Cuts at:   1802    1802 
   Size:       3623
 
NspI r_CATG'y

Cuts at:    676    1176    2083     676 
   Size:        500     907    2216

  Fragments arranged by size: 

                2216     907     500
 
PasI CC'CwG_GG

Cuts at:    153     267     290     153 
   Size:        114      23    3486

  Fragments arranged by size: 

                3486     114      23
 
PciI A'CATG_T

Cuts at:   2079    2079 
   Size:       3623
 
PflMI CCAn_nnn'nTGG

Cuts at:    554     554 
   Size:       3623
 
PfoI T'CCnGG_A

Cuts at:    506     514     506 
   Size:          8    3615
 
PmeI GTTT'AAAC

Cuts at:    622     622 
   Size:       3623
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2762    2794    2762 
   Size:         32    3591
 
PstI C_TGCA'G

Cuts at:    670    1355     670 
   Size:        685    2938
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3623
 
PvuII CAG'CTG

Cuts at:    261    1048    1408     261 
   Size:        787     360    2476

  Fragments arranged by size: 

                2476     787     360
 
RsrII CG'GwC_CG

Cuts at:   1818    1818 
   Size:       3623
 
SacI G_AGCT'C

Cuts at:    637     637 
   Size:       3623
 
SalI G'TCGA_C

Cuts at:    660     660 
   Size:       3623
 
SapI GCTCTTCn'nnn_

Cuts at:    476     537    1646    1856     476 
   Size:         61    1109     210    2243

  Fragments arranged by size: 

                2243    1109     210      61
 
SbfI CC_TGCA'GG

Cuts at:    670     670 
   Size:       3623
 
ScaI AGT'ACT

Cuts at:   3140    3140 
   Size:       3623
 
SfcI C'TryA_G

Cuts at:     33     666    1351    2344    2535      33 
   Size:        633     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     633     191
 
SfoI GGC'GCC

Cuts at:    575    1055    1303     575 
   Size:        480     248    2895

  Fragments arranged by size: 

                2895     480     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3623
 
SgrAI Cr'CCGG_yG

Cuts at:    341     341 
   Size:       3623
 
SmaI CCC'GGG

Cuts at:    239     366     645     239 
   Size:        127     279    3217

  Fragments arranged by size: 

                3217     279     127
 
SmlI C'TyrA_G

Cuts at:    778    1693    2185    2447     778 
   Size:        915     492     262    1954

  Fragments arranged by size: 

                1954     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3623
 
SphI G_CATG'C

Cuts at:    676    1176     676 
   Size:        500    3123
 
StyI C'CwwG_G

Cuts at:    107     757    1111     107 
   Size:        650     354    2619

  Fragments arranged by size: 

                2619     650     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1206    1339    1798    2007    1206 
   Size:        133     459     209    2822

  Fragments arranged by size: 

                2822     459     209     133
 
TatI w'GTAC_w

Cuts at:   3138    3138 
   Size:       3623
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3140    3172    3140 
   Size:         32    3591
 
Tth111I GACn'n_nGTC

Cuts at:   1420    1420 
   Size:       3623
 
XbaI T'CTAG_A

Cuts at:    654     654 
   Size:       3623
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    258     483    1118     258 
   Size:        225     635    2763

  Fragments arranged by size: 

                2763     635     225
 
XmaI C'CCGG_G

Cuts at:    237     364     643     237 
   Size:        127     279    3217

  Fragments arranged by size: 

                3217     279     127
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AhdI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI     BplI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI    BseYI
    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstYI     BtgI
    BtgZI     BtsI     DraI     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI    HaeII
    Hin4I   HincII     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PasI
     PciI    PflMI     PfoI     PmeI     PpiI     PstI     PvuI    PvuII
    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI     SfoI
     SgfI    SgrAI     SmaI     SmlI     SpeI     SphI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AleI     AlfI     AloI     ApaI
     AscI    AvrII     BaeI     BbsI    BbvCI     BcgI    BfrBI     BglI
    BmgBI     BmtI     BpmI   Bpu10I    BsaXI     BsgI    BsiWI    BsrGI
   BssHII   BstEII    BstXI  BstZ17I   Bsu36I     ClaI    CspCI   DraIII
    EcoRV     FseI    FspAI  HindIII     HpaI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PmlI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI    SacII    SanDI    SexAI     SfiI    SnaBI     SrfI
     SspI     StuI     SwaI     XhoI     XmnI     ZraI
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