Restriction map for pF1KE0593
##################################
# NO CUT ENZYMES (  162 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AcsI        
AflII       AflIII      AgeI        AhdI        Alw44I      
Aor51HI     ApaLI       ApoI        AscI        AseI        
AsnI        Asp718I     AspEI       AspHI       AspI        
AvaI        AviII       AvrII       BalI        BanI        
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BclI        BcnI        BfaI        BfrI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaI        BsaWI       BseAI       BsiHKAI     BsiWI       
BsmBI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstZ17I     
CelII       Cfr10I      ClaI        CpoI        DraI        
DraIII      DrdI        DsaI        Eam1105I    Eco47III    
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HapII       HgaI        HincII      
HindII      HindIII     HinfI       HpaI        HpaII       
KasI        KpnI        KspI        MaeI        MaeIII      
MfeI        MluI        MluNI       MroI        MscI        
MspA1I      MspI        MunI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfcI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TfiI        
Tsp45I      Tsp509I     Tth111I     XbaI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AatII         585  GACGTC               GACGT^C
AcyI          585  GACGTC               G[GA]^CG[CT]C
AfaI          466  GTAC                 GT^AC
ApaI          374  GGGCCC               GGGCC^C
Asp700I       647  GAAGCTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII         398  GGACC                G^G[AT]CC
BamHI         227  GGATCC               G^GATCC
BciVI         194  GTATCC               GTATCC(6/5)
BsaBI         228  GATCCACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         585  GACGTC               G[GA]^CG[CT]C
BseRI         116  CTCCTC               GAGGAG(10/8)
BsiEI         173  CGGCCG               CG[GA][CT]^CG
BsmAI         405  GAGAC                GTCTC(1/5)
BsmFI         707  GGGAC                GGGAC(10/14)
BsrI          419  ACTGG                ACTGG(1/-1)
BstYI         227  GGATCC               [GA]^GATC[CT]
EaeI          173  CGGCCG               [CT]^GGCC[GA]
EagI          173  CGGCCG               C^GGCCG
EarI          439  GAAGAG               CTCTTC(1/4)
EclXI         173  CGGCCG               C^GGCCG
Eco52I        173  CGGCCG               C^GGCCG
EcoT14I       702  CCAAGG               C^C[AT][AT]GG
FauI          639  CCCGC                CCCGC(4/6)
HaeII         142  GGCGCT               [GA]GCGC^[CT]
Hin1I         585  GACGTC               G[GA]^CG[CT]C
Ksp632I       439  GAAGAG               CTCTTC(1/4)
MamI          228  GATCCACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          227  GGATCC               [GA]^GATC[CT]
MseI          416  TTAA                 T^TAA
NlaIII        684  CATG                 CATG^
PflMI         364  CCAACAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsaI          466  GTAC                 GT^AC
SfaNI          44  GATGC                GCATC(5/9)
StyI          702  CCAAGG               C^C[AT][AT]GG
TaqI          645  TCGA                 T^CGA
Tru9I         416  TTAA                 T^TAA
TthHB8I       645  TCGA                 T^CGA
Van91I        364  CCAACAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          522  CCAGAACTCAAATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         227  GGATCC               [GA]^GATC[CT]
XmnI          647  GAAGCTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AccII         171  CGCG                 CG^CG
              463  CGCG                
AciI          640  CCGC                 CCGC(-3/-1)
              172  GCGG                
AlwI          228  GATCC                GGATC(4/5)
              227  GGATC               
AlwNI         140  CAGGCGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              485  CAGCACCTG           
AocI          160  CCTAAGG              CC^T[ACGT]AGG
              313  CCTCAGG             
BpmI          626  CTCCAG               CTGGAG(16/14)
              402  CTGGAG              
BsgI          200  CTGCAC               GTGCAG(16/14)
              571  CTGCAC              
BspMI          79  ACCTGC               ACCTGC(4/8)
              335  GCAGGT              
BstUI         171  CGCG                 CG^CG
              463  CGCG                
BstXI          24  CCAGCAAGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              331  CCAGGCAGGTGG        
Bsu36I        160  CCTAAGG              CC^T[ACGT]AGG
              313  CCTCAGG             
DpnII         228  GATC                 ^GATC
              281  GATC                
Eco81I        160  CCTAAGG              CC^T[ACGT]AGG
              313  CCTCAGG             
HphI           77  TCACC                GGTGA(8/7)
              554  TCACC               
MaeII         563  ACGT                 A^CGT
              586  ACGT                
MboI          228  GATC                 ^GATC
              281  GATC                
MslI          682  CACATGGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              684  CATGGGTGTG          
MvnI          171  CGCG                 CG^CG
              463  CGCG                
NdeII         228  GATC                 ^GATC
              281  GATC                
Sau3AI        228  GATC                 ^GATC
              281  GATC                


#########################################
# 3 OR MORE CUT ENZYMES (   35 enzymes) #
#########################################

AluI        BanII       BbvI        BglI        BmyI        
BsaJI       BsiYI       BslI        Bsp1286I    BssKI       
BstF5I      BstNI       Cac8I       CfoI        Cfr13I      
DdeI        DraII       EcoNI       EcoO109I    EcoRII      
Fnu4HI      FokI        HaeIII      HhaI        HinP1I      
ItaI        MboII       MnlI        MvaI        MwoI        
NlaIV       Sau96I      ScrFI       TseI        TspRI       
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