Restriction map for pF1KE0593
 (Linear) (Six-base) MAPSORT of: KIEE0593.seq  Check: 8981  from: 1  to: 741



KIEE0593.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:16  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      88     326     741 
   Size:         88     238     415

  Fragments arranged by size: 

                 415     238      88
 
AatII G_ACGT'C

Cuts at:      0     589     741 
   Size:        589     152
 
AleI CACnn'nnGTG

Cuts at:      0     686     741 
   Size:        686      55
 
AlwNI CAG_nnn'CTG

Cuts at:      0     145     490     741 
   Size:        145     345     251

  Fragments arranged by size: 

                 345     251     145
 
ApaI G_GGCC'C

Cuts at:      0     378     741 
   Size:        378     363
 
BamHI G'GATC_C

Cuts at:      0     227     741 
   Size:        227     514
 
BanII G_rGCy'C

Cuts at:      0     103     378     627     741 
   Size:        103     275     249     114

  Fragments arranged by size: 

                 275     249     114     103
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     205     741 
   Size:        205     536
 
BglI GCCn_nnn'nGGC

Cuts at:      0     181     265     622     741 
   Size:        181      84     357     119

  Fragments arranged by size: 

                 357     181     119      84
 
Bme1580I G_kGCm'C

Cuts at:      0       7      38     297     378     479     741 
   Size:          7      31     259      81     101     262

  Fragments arranged by size: 

                 262     259     101      81      31       7
 
BmgBI CAC'GTC

Cuts at:      0     564     741 
   Size:        564     177
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     429     741 
   Size:        429     312
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     423     611     741 
   Size:        423     188     130

  Fragments arranged by size: 

                 423     188     130
 
BsaBI GATnn'nnATC

Cuts at:      0     232     741 
   Size:        232     509
 
BsaHI Gr'CG_yC

Cuts at:      0     586     741 
   Size:        586     155
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     527     557     741 
   Size:        527      30     184

  Fragments arranged by size: 

                 527     184      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     107     741 
   Size:        107     634
 
BseYI C'CCAG_C

Cuts at:      0      31     129     741 
   Size:         31      98     612

  Fragments arranged by size: 

                 612      98      31
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     185     556     741 
   Size:        185     371     185

  Fragments arranged by size: 

                 371     185     185
 
BsiEI CG_ry'CG

Cuts at:      0     176     741 
   Size:        176     565
 
Bsp1286I G_dGCh'C

Cuts at:      0       7      38     103     297     378     479     627     741 
   Size:          7      31      65     194      81     101     148     114

  Fragments arranged by size: 

                 194     148     114     101      81      65      31       7
            
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      88     326     741 
   Size:         88     238     415

  Fragments arranged by size: 

                 415     238      88
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      31     338     741 
   Size:         31     307     403

  Fragments arranged by size: 

                 403     307      31
 
BstYI r'GATC_y

Cuts at:      0     227     741 
   Size:        227     514
 
Bsu36I CC'TnA_GG

Cuts at:      0     161     314     741 
   Size:        161     153     427

  Fragments arranged by size: 

                 427     161     153
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      57     741 
   Size:         57     684
 
EaeI y'GGCC_r

Cuts at:      0     173     741 
   Size:        173     568
 
EagI C'GGCC_G

Cuts at:      0     173     741 
   Size:        173     568
 
EarI CTCTTCn'nnn_

Cuts at:      0     434     741 
   Size:        434     307
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     423     611     741 
   Size:        423     188     130

  Fragments arranged by size: 

                 423     188     130
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     122     301     313     741 
   Size:        122     179      12     428

  Fragments arranged by size: 

                 428     179     122      12
 
EcoO109I rG'GnC_Cy

Cuts at:      0     306     323     374     741 
   Size:        306      17      51     367

  Fragments arranged by size: 

                 367     306      51      17
 
HaeII r_GCGC'y

Cuts at:      0     146     741 
   Size:        146     595
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     102     134     527     559     741 
   Size:        102      32     393      32     182

  Fragments arranged by size: 

                 393     182     102      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     388     741 
   Size:        388     353
 
MslI CAynn'nnrTG

Cuts at:      0     686     688     741 
   Size:        686       2      53

  Fragments arranged by size: 

                 686      53       2
 
PasI CC'CwG_GG

Cuts at:      0     262     741 
   Size:        262     479
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     370     741 
   Size:        370     371
 
PspOMI G'GGCC_C

Cuts at:      0     374     741 
   Size:        374     367
 
StyI C'CwwG_G

Cuts at:      0     702     741 
   Size:        702      39
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     530     676     741 
   Size:        530     146      65

  Fragments arranged by size: 

                 530     146      65
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     529     741 
   Size:        529     212
 
XmnI GAAnn'nnTTC

Cuts at:      0     651     741 
   Size:        651      90
 
ZraI GAC'GTC

Cuts at:      0     587     741 
   Size:        587     154
 

 Enzymes that do cut:

     AarI    AatII     AleI    AlwNI     ApaI    BamHI    BanII    BciVI
     BglI Bme1580I    BmgBI     BmrI     BpmI    BsaBI    BsaHI    BsaXI
    BseRI    BseYI     BsgI    BsiEI Bsp1286I    BspMI    BstXI    BstYI
   Bsu36I    BtgZI     EaeI     EagI     EarI  Eco57MI    EcoNI EcoO109I
    HaeII    Hin4I     MmeI     MslI     PasI    PflMI   PspOMI     StyI
    TaqII     XcmI     XmnI     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI     BanI     BbeI     BbsI    BbvCI     BcgI
     BclI    BfrBI    BglII     BlpI     BmtI     BplI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaWI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII  BstZ17I     BtgI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EciI  EcoICRI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PciI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI     TatI     TstI  Tth111I     XbaI     XhoI     XmaI
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