Restriction map for pF1KE0581
 (Linear) (Six-base) MAPSORT of: KIEE0581.seq  Check: 1893  from: 1  to: 849



KIEE0581.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:16  ..

AfeI AGC'GCT

Cuts at:      0      47     849 
   Size:         47     802
 
AleI CACnn'nnGTG

Cuts at:      0     430     849 
   Size:        430     419
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     442     474     849 
   Size:        442      32     375

  Fragments arranged by size: 

                 442     375      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     624     822     849 
   Size:        624     198      27

  Fragments arranged by size: 

                 624     198      27
 
ApaI G_GGCC'C

Cuts at:      0     439     849 
   Size:        439     410
 
BamHI G'GATC_C

Cuts at:      0     269     849 
   Size:        269     580
 
BanII G_rGCy'C

Cuts at:      0     338     439     701     743     849 
   Size:        338     101     262      42     106

  Fragments arranged by size: 

                 338     262     106     101      42
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      90     849 
   Size:         90     759
 
BclI T'GATC_A

Cuts at:      0     608     849 
   Size:        608     241
 
BlpI GC'TnA_GC

Cuts at:      0     643     849 
   Size:        643     206
 
Bme1580I G_kGCm'C

Cuts at:      0     439     512     849 
   Size:        439      73     337

  Fragments arranged by size: 

                 439     337      73
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      55     156     331     849 
   Size:         55     101     175     518

  Fragments arranged by size: 

                 518     175     101      55
 
Bpu10I CC'TnA_GC

Cuts at:      0     727     849 
   Size:        727     122
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     412     849 
   Size:        412     437
 
BsaWI w'CCGG_w

Cuts at:      0     191     849 
   Size:        191     658
 
BseYI C'CCAG_C

Cuts at:      0     329     490     751     849 
   Size:        329     161     261      98

  Fragments arranged by size: 

                 329     261     161      98
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     300     408     610     849 
   Size:        300     108     202     239

  Fragments arranged by size: 

                 300     239     202     108
 
BsiHKAI G_wGCw'C

Cuts at:      0     185     338     849 
   Size:        185     153     511

  Fragments arranged by size: 

                 511     185     153
 
Bsp1286I G_dGCh'C

Cuts at:      0     185     338     439     512     701     743     849 
   Size:        185     153     101      73     189      42     106

  Fragments arranged by size: 

                 189     185     153     106     101      73      42
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     430     849 
   Size:        430     419
 
BsrFI r'CCGG_y

Cuts at:      0     665     849 
   Size:        665     184
 
BssHII G'CGCG_C

Cuts at:      0     681     849 
   Size:        681     168
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     624     849 
   Size:        624     225
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     576     759     849 
   Size:        576     183      90

  Fragments arranged by size: 

                 576     183      90
 
BstYI r'GATC_y

Cuts at:      0     269     359     849 
   Size:        269      90     490

  Fragments arranged by size: 

                 490     269      90
 
BtgI C'CryG_G

Cuts at:      0      59     849 
   Size:         59     790
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     687     849 
   Size:        687     162
 
ClaI AT'CG_AT

Cuts at:      0     161     849 
   Size:        161     688
 
EaeI y'GGCC_r

Cuts at:      0     530     581     849 
   Size:        530      51     268

  Fragments arranged by size: 

                 530     268      51
 
EcoICRI GAG'CTC

Cuts at:      0     336     849 
   Size:        336     513
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      55     156     331     849 
   Size:         55     101     175     518

  Fragments arranged by size: 

                 518     175     101      55
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     349     849 
   Size:        349     500
 
EcoO109I rG'GnC_Cy

Cuts at:      0     436     789     849 
   Size:        436     353      60

  Fragments arranged by size: 

                 436     353      60
 
HaeII r_GCGC'y

Cuts at:      0      49     758     849 
   Size:         49     709      91

  Fragments arranged by size: 

                 709      91      49
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     154     186     849 
   Size:        154      32     663

  Fragments arranged by size: 

                 663     154      32
 
MscI TGG'CCA

Cuts at:      0     532     583     849 
   Size:        532      51     266

  Fragments arranged by size: 

                 532     266      51
 
MslI CAynn'nnrTG

Cuts at:      0     430     469     652     849 
   Size:        430      39     183     197

  Fragments arranged by size: 

                 430     197     183      39
 
MspA1I CmG'CkG

Cuts at:      0     329     822     849 
   Size:        329     493      27

  Fragments arranged by size: 

                 493     329      27
 
NcoI C'CATG_G

Cuts at:      0      59     849 
   Size:         59     790
 
NspI r_CATG'y

Cuts at:      0     324     639     849 
   Size:        324     315     210

  Fragments arranged by size: 

                 324     315     210
 
PasI CC'CwG_GG

Cuts at:      0     672     849 
   Size:        672     177
 
PfoI T'CCnGG_A

Cuts at:      0     101     347     849 
   Size:        101     246     502

  Fragments arranged by size: 

                 502     246     101
 
PpuMI rG'GwC_Cy

Cuts at:      0     789     849 
   Size:        789      60
 
PspOMI G'GGCC_C

Cuts at:      0     435     849 
   Size:        435     414
 
PstI C_TGCA'G

Cuts at:      0     620     849 
   Size:        620     229
 
PvuII CAG'CTG

Cuts at:      0     329     849 
   Size:        329     520
 
SacI G_AGCT'C

Cuts at:      0     338     849 
   Size:        338     511
 
SfcI C'TryA_G

Cuts at:      0     616     849 
   Size:        616     233
 
SphI G_CATG'C

Cuts at:      0     639     849 
   Size:        639     210
 
StyI C'CwwG_G

Cuts at:      0      59     849 
   Size:         59     790
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     188     220     849 
   Size:        188      32     629

  Fragments arranged by size: 

                 629     188      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     145     849 
   Size:        145     704
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     576     669     849 
   Size:        576      93     180

  Fragments arranged by size: 

                 576     180      93
 

 Enzymes that do cut:

     AfeI     AleI     AloI    AlwNI     ApaI    BamHI    BanII     BbsI
     BclI     BlpI Bme1580I     BpmI   Bpu10I     BsaI    BsaWI    BseYI
     BsgI  BsiHKAI Bsp1286I    BspMI    BsrFI   BssHII   BstAPI    BstXI
    BstYI     BtgI    BtgZI     ClaI     EaeI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    HaeII    Hin4I     MscI     MslI   MspA1I     NcoI     NspI
     PasI     PfoI    PpuMI   PspOMI     PstI    PvuII     SacI     SfcI
     SphI     StyI     TstI  Tth111I     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AlfI    ApaLI     ApoI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI     BanI     BbeI    BbvCI     BcgI    BciVI
    BfrBI     BglI    BglII    BmgBI     BmrI     BmtI     BplI    BpuEI
    BsaAI    BsaBI    BsaHI    BsaXI    BseRI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrGI    BssSI    BstBI
   BstEII  BstZ17I   Bsu36I     BtsI    CspCI     DraI   DraIII     DrdI
     EagI     EarI     EciI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PciI    PflMI     PmeI     PmlI     PpiI    PshAI
     PsiI    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SrfI     SspI     StuI     SwaI
    TaqII     TatI     XbaI     XhoI     XmaI     XmnI     ZraI
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