Restriction map for pF1KE0569
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# NO CUT ENZYMES (  196 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AgeI        AhdI        Alw44I      
AlwI        AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BclI        BcnI        BfrI        BglI        BglII       
BlnI        BlpI        BmyI        BpmI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsaI        BsaJI       
BsaWI       BseAI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmAI       BsmBI       BsoBI       Bsp1286I    
Bsp1407I    BspDI       BspEI       BspHI       BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssKI       BssSI       Bst1107I    BstEII      BstNI       
BstPI       BstUI       BstXI       BstYI       BstZ17I     
Bsu36I      Cac8I       CelII       CfoI        Cfr10I      
ClaI        CpoI        DraI        DraII       DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRII      
EcoRV       EcoT14I     EcoT22I     FauI        FbaI        
FseI        FspI        HaeII       HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HpaI        HpaII       KasI        KpnI        Ksp632I     
KspI        MaeII       MamI        MflI        MluI        
MluNI       MroI        MscI        MspA1I      MspI        
MvaI        MvnI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
ScrFI       SexAI       SfiI        SfoI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        StyI        
SwaI        Tsp45I      Tth111I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


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# ONE CUT ENZYMES (   38 enzymes) #
###################################

AccI          125  GTAGAC               GT^[AC][GT]AC
AciI          381  CCGC                 CCGC(-3/-1)
AcsI          475  GAATTT               [GA]^AATT[CT]
AflIII        540  ACATGT               A^C[GA][CT]GT
ApoI          475  GAATTT               [GA]^AATT[CT]
AvaII         378  GGACC                G^G[AT]CC
BbsI            3  GTCTTC               GAAGAC(2/6)
BbvI          319  GCAGC                GCAGC(8/12)
BpuAI           3  GTCTTC               GAAGAC(2/6)
BseRI           8  CTCCTC               GAGGAG(10/8)
BsiYI         169  CCTAGATATGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          169  CCTAGATATGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI         377  GGGAC                GGGAC(10/14)
BsmI           62  GCATTC               GAATGC(1/-1)
BspLU11I      540  ACATGT               A^CATGT
BsrI          199  ACTGG                ACTGG(1/-1)
BstAPI        138  GCAGGATATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         360  TTCGAA               TT^CGAA
DpnII         240  GATC                 ^GATC
Fnu4HI        319  GCAGC                GC^[ACGT]GC
HaeIII        167  GGCC                 GG^CC
HindIII       356  AAGCTT               A^AGCTT
HphI          242  TCACC                GGTGA(8/7)
ItaI          319  GCAGC                GC^[ACGT]GC
MaeIII        519  GTTAC                ^GT[ACGT]AC
MboI          240  GATC                 ^GATC
MslI          259  CACATTTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeII         240  GATC                 ^GATC
NlaIV         377  GGGACC               GG[ACGT]^[ACGT]CC
NspV          360  TTCGAA               TT^CGAA
Sau3AI        240  GATC                 ^GATC
SfaNI         289  GATGC                GCATC(5/9)
SfcI          332  CTATAG               C^T[GA][CT]AG
SfuI          360  TTCGAA               TT^CGAA
TaqI          361  TCGA                 T^CGA
TfiI           31  GATTC                G^A[AT]TC
TseI          319  GCAGC                G^C[AT]GC
TthHB8I       361  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AfaI          397  GTAC                 GT^AC
              538  GTAC                
AlwNI         151  CAGAAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              368  CAGGTGCTG           
BstF5I         99  GGATG                GGATG(2/0)
              489  GGATG               
Cfr13I        166  GGGCC                G^G[ACGT]CC
              378  GGACC               
FokI           99  GGATG                GGATG(9/13)
              489  GGATG               
MfeI          221  CAATTG               C^AATTG
              248  CAATTG              
MunI          221  CAATTG               C^AATTG
              248  CAATTG              
MwoI          138  GCAGGATATGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              147  GCACCAGAAGC         
NlaIII        311  CATG                 CATG^
              541  CATG                
NspI          310  ACATGC               [GA]CATG^[CT]
              540  ACATGT              
PleI           44  GAGTC                GAGTC(4/5)
              496  GAGTC               
RsaI          397  GTAC                 GT^AC
              538  GTAC                
Sau96I        166  GGGCC                G^G[ACGT]CC
              378  GGACC               
TspRI         130  CAGTG                CA[GC]TG[ACGT][ACGT]^
              407  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (   10 enzymes) #
#########################################

AluI        BfaI        DdeI        HinfI       MaeI        
MboII       MnlI        MseI        Tru9I       Tsp509I     
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