################################## # NO CUT ENZYMES ( 184 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I CelII Cfr13I ClaI CpoI DpnII DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI HphI KasI KpnI KspI MaeII MaeIII MamI MboI MfeI MflI MluI MluNI MroI MscI MseI MunI MvnI NarI NciI NcoI NdeI NdeII NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI Sau3AI Sau96I SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI TaqI TfiI Tru9I Tsp45I Tsp509I Tth111I TthHB8I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 28 enzymes) # ################################### AfaI 595 GTAC GT^AC AocI 743 CCTCAGG CC^T[ACGT]AGG BanI 94 GGCACC G^G[CT][GA]CC BglI 200 GCCAATCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 27 CTCCAG CTGGAG(16/14) BsaJI 131 CCCTGG C^C[ACGT][ACGT]GG BsmFI 693 GTCCC GGGAC(10/14) BsrI 145 CCAGT ACTGG(1/-1) BssSI 231 CTCGTG CACGAG(-5/-1) BstF5I 473 CATCC GGATG(2/0) Bsu36I 743 CCTCAGG CC^T[ACGT]AGG EaeI 2 TGGCCG [CT]^GGCC[GA] Eco81I 743 CCTCAGG CC^T[ACGT]AGG FauI 719 CCCGC CCCGC(4/6) FokI 473 CATCC GGATG(9/13) HinfI 469 GAGTC G^A[ACGT]TC MslI 141 CACCCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 102 CAGCTG C[AC]G^C[GT]G NlaIV 94 GGCACC GG[ACGT]^[ACGT]CC PflMI 41 CCAACTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 469 GAGTC GAGTC(4/5) PshAI 129 GACCCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 102 CAGCTG CAG^CTG RsaI 595 GTAC GT^AC SacI 82 GAGCTC GAGCT^C StuI 290 AGGCCT AGG^CCT TspRI 146 CAGTG CA[GC]TG[ACGT][ACGT]^ Van91I 41 CCAACTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AspHI 82 GAGCTC G[AT]GC[AT]^C 714 GTGCTC BanII 82 GAGCTC G[GA]GC[CT]^C 187 GAGCCC BsiHKAI 82 GAGCTC G[AT]GC[AT]^C 714 GTGCTC BsiYI 41 CCAACTCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 126 CCTGACCCTGG BslI 41 CCAACTCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 126 CCTGACCCTGG BspMI 460 ACCTGC ACCTGC(4/8) 51 GCAGGT BsrFI 258 GCCGGC [GA]^CCGG[CT] 507 GCCGGC CfoI 32 GCGC GCG^C 194 GCGC Cfr10I 258 GCCGGC [GA]^CCGG[CT] 507 GCCGGC DdeI 467 CTGAG C^T[ACGT]AG 744 CTCAG EarI 396 CTCTTC CTCTTC(1/4) 477 CTCTTC HaeII 31 AGCGCC [GA]GCGC^[CT] 193 AGCGCC HapII 259 CCGG C^CGG 508 CCGG HhaI 32 GCGC GCG^C 194 GCGC HinP1I 32 GCGC G^CGC 194 GCGC HpaII 259 CCGG C^CGG 508 CCGG Ksp632I 396 CTCTTC CTCTTC(1/4) 477 CTCTTC MspI 259 CCGG C^CGG 508 CCGG NaeI 258 GCCGGC GCC^GGC 507 GCCGGC NgoMIV 258 GCCGGC G^CCGGC 507 GCCGGC PstI 672 CTGCAG CTGCA^G 732 CTGCAG SfcI 672 CTGCAG C^T[GA][CT]AG 732 CTGCAG ######################################### # 3 OR MORE CUT ENZYMES ( 24 enzymes) # ######################################### AciI AluI AlwNI BbvI BfaI BmyI BseRI BsgI Bsp1286I BssKI BstNI Cac8I EcoRII Fnu4HI HaeIII ItaI MaeI MboII MnlI MvaI MwoI NlaIII ScrFI TseI