Restriction map for pF1KE0564
 (Linear) (Six-base) MAPSORT of: KIEE0564.seq  Check: 85  from: 1  to: 1344



KIEE0564.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:16  ..

AatII G_ACGT'C

Cuts at:      0    1154    1344 
   Size:       1154     190
 
AclI AA'CG_TT

Cuts at:      0    1079    1344 
   Size:       1079     265
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     525     688     898    1344 
   Size:        525     163     210     446

  Fragments arranged by size: 

                 525     446     210     163
 
AflIII A'CryG_T

Cuts at:      0    1181    1344 
   Size:       1181     163
 
AhdI GACnn_n'nnGTC

Cuts at:      0     429     952    1344 
   Size:        429     523     392

  Fragments arranged by size: 

                 523     429     392
 
AleI CACnn'nnGTG

Cuts at:      0    1258    1344 
   Size:       1258      86
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     377    1344 
   Size:        377     967
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     841     873    1344 
   Size:        841      32     471

  Fragments arranged by size: 

                 841     471      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     209    1143    1344 
   Size:        209     934     201

  Fragments arranged by size: 

                 934     209     201
 
ApaI G_GGCC'C

Cuts at:      0     247    1161    1344 
   Size:        247     914     183

  Fragments arranged by size: 

                 914     247     183
 
BamHI G'GATC_C

Cuts at:      0     380     611    1344 
   Size:        380     231     733

  Fragments arranged by size: 

                 733     380     231
 
BanII G_rGCy'C

Cuts at:      0     247     458    1161    1344 
   Size:        247     211     703     183

  Fragments arranged by size: 

                 703     247     211     183
 
BclI T'GATC_A

Cuts at:      0    1070    1344 
   Size:       1070     274
 
BglI GCCn_nnn'nGGC

Cuts at:      0     526    1093    1334    1344 
   Size:        526     567     241      10

  Fragments arranged by size: 

                 567     526     241      10
 
Bme1580I G_kGCm'C

Cuts at:      0     247    1161    1265    1280    1344 
   Size:        247     914     104      15      64

  Fragments arranged by size: 

                 914     247     104      64      15
 
BmgBI CAC'GTC

Cuts at:      0     846    1344 
   Size:        846     498
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     370     391     442     667     814     985    1135    1186 
   Size:        370      21      51     225     147     171     150      51

Cuts at:   1186    1219    1344 
   Size:         33     125

  Fragments arranged by size: 

                 370     225     171     150     147     125      51      51
                  33      21
 
Bpu10I CC'TnA_GC

Cuts at:      0      98    1344 
   Size:         98    1246
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      28    1344 
   Size:         28    1316
 
BsaAI yAC'GTr

Cuts at:      0    1184    1344 
   Size:       1184     160
 
BsaHI Gr'CG_yC

Cuts at:      0    1151    1344 
   Size:       1151     193
 
BsaWI w'CCGG_w

Cuts at:      0    1187    1344 
   Size:       1187     157
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     402     432     667     697     790     820     841     871 
   Size:        402      30     235      30      93      30      21      30

Cuts at:    871    1219    1249    1344 
   Size:        348      30      95

  Fragments arranged by size: 

                 402     348     235      95      93      30      30      30
                  30      30      21
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     466     688     718     811     889    1344 
   Size:        466     222      30      93      78     455

  Fragments arranged by size: 

                 466     455     222      93      78      30
 
BseYI C'CCAG_C

Cuts at:      0     983    1046    1088    1344 
   Size:        983      63      42     256

  Fragments arranged by size: 

                 983     256      63      42
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     749    1233    1344 
   Size:        749     484     111

  Fragments arranged by size: 

                 749     484     111
 
BsiEI CG_ry'CG

Cuts at:      0     315    1344 
   Size:        315    1029
 
BsiHKAI G_wGCw'C

Cuts at:      0     410     458    1344 
   Size:        410      48     886

  Fragments arranged by size: 

                 886     410      48
 
BsmI GAATG_Cn'

Cuts at:      0     366     495    1344 
   Size:        366     129     849

  Fragments arranged by size: 

                 849     366     129
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     939    1344 
   Size:        939     405
 
Bsp1286I G_dGCh'C

Cuts at:      0     247     410     458    1161    1265    1280    1344 
   Size:        247     163      48     703     104      15      64

  Fragments arranged by size: 

                 703     247     163     104      64      48      15
 
BsrBI CCG'CTC

Cuts at:      0    1119    1344 
   Size:       1119     225
 
BsrDI GCAATG_nn'

Cuts at:      0     288    1344 
   Size:        288    1056
 
BsrFI r'CCGG_y

Cuts at:      0      92    1344 
   Size:         92    1252
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     490    1316    1344 
   Size:        490     826      28

  Fragments arranged by size: 

                 826     490      28
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     792    1344 
   Size:        792     552
 
BstYI r'GATC_y

Cuts at:      0     380     611    1100    1344 
   Size:        380     231     489     244

  Fragments arranged by size: 

                 489     380     244     231
 
Bsu36I CC'TnA_GG

Cuts at:      0     994    1344 
   Size:        994     350
 
BtgI C'CryG_G

Cuts at:      0     128     239     821    1344 
   Size:        128     111     582     523

  Fragments arranged by size: 

                 582     523     128     111
 
BtsI GCAGTG_nn'

Cuts at:      0    1069    1344 
   Size:       1069     275
 
DraIII CAC_nnn'GTG

Cuts at:      0     632    1344 
   Size:        632     712
 
EaeI y'GGCC_r

Cuts at:      0     169     320     566     893    1344 
   Size:        169     151     246     327     451

  Fragments arranged by size: 

                 451     327     246     169     151
 
EcoICRI GAG'CTC

Cuts at:      0     456    1344 
   Size:        456     888
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     370     391     442     525     667     688     814     898 
   Size:        370      21      51      83     142      21     126      84

Cuts at:    898     985    1135    1186    1219    1344 
   Size:         87     150      51      33     125

  Fragments arranged by size: 

                 370     150     142     126     125      87      84      83
                  51      51      33      21      21
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     670    1344 
   Size:        670     674
 
EcoO109I rG'GnC_Cy

Cuts at:      0     244     269    1344 
   Size:        244      25    1075

  Fragments arranged by size: 

                1075     244      25
 
FspI TGC'GCA

Cuts at:      0     607    1344 
   Size:        607     737
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     415     447     792     824     938     970    1344 
   Size:        415      32     345      32     114      32     374

  Fragments arranged by size: 

                 415     374     345     114      32      32      32
 
HincII GTy'rAC

Cuts at:      0     957    1344 
   Size:        957     387
 
MscI TGG'CCA

Cuts at:      0     322     568     895    1344 
   Size:        322     246     327     449

  Fragments arranged by size: 

                 449     327     322     246
 
MslI CAynn'nnrTG

Cuts at:      0     754    1258    1344 
   Size:        754     504      86

  Fragments arranged by size: 

                 754     504      86
 
MspA1I CmG'CkG

Cuts at:      0      80     179     254     348     579    1050    1092    1344 
   Size:         80      99      75      94     231     471      42     252

  Fragments arranged by size: 

                 471     252     231      99      94      80      75      42
            
 
NaeI GCC'GGC

Cuts at:      0      94    1344 
   Size:         94    1250
 
NcoI C'CATG_G

Cuts at:      0     128     239     821    1344 
   Size:        128     111     582     523

  Fragments arranged by size: 

                 582     523     128     111
 
NgoMIV G'CCGG_C

Cuts at:      0      92    1344 
   Size:         92    1252
 
NspI r_CATG'y

Cuts at:      0     137    1314    1344 
   Size:        137    1177      30

  Fragments arranged by size: 

                1177     137      30
 
PasI CC'CwG_GG

Cuts at:      0    1332    1344 
   Size:       1332      12
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     129     891    1344 
   Size:        129     762     453

  Fragments arranged by size: 

                 762     453     129
 
PfoI T'CCnGG_A

Cuts at:      0     614    1344 
   Size:        614     730
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     841     873    1344 
   Size:        841      32     471

  Fragments arranged by size: 

                 841     471      32
 
PspOMI G'GGCC_C

Cuts at:      0     243    1157    1344 
   Size:        243     914     187

  Fragments arranged by size: 

                 914     243     187
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1117    1149    1344 
   Size:       1117      32     195

  Fragments arranged by size: 

                1117     195      32
 
PstI C_TGCA'G

Cuts at:      0     980    1344 
   Size:        980     364
 
PvuII CAG'CTG

Cuts at:      0     179     254     348     579    1050    1092    1344 
   Size:        179      75      94     231     471      42     252

  Fragments arranged by size: 

                 471     252     231     179      94      75      42
 
SacI G_AGCT'C

Cuts at:      0     458    1344 
   Size:        458     886
 
SexAI A'CCwGG_T

Cuts at:      0    1136    1344 
   Size:       1136     208
 
SfcI C'TryA_G

Cuts at:      0     976    1038    1344 
   Size:        976      62     306

  Fragments arranged by size: 

                 976     306      62
 
SmlI C'TyrA_G

Cuts at:      0      43    1344 
   Size:         43    1301
 
SphI G_CATG'C

Cuts at:      0     137    1314    1344 
   Size:        137    1177      30

  Fragments arranged by size: 

                1177     137      30
 
StuI AGG'CCT

Cuts at:      0     391    1344 
   Size:        391     953
 
StyI C'CwwG_G

Cuts at:      0     128     239     479     821    1344 
   Size:        128     111     240     342     523

  Fragments arranged by size: 

                 523     342     240     128     111
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      57     135    1344 
   Size:         57      78    1209

  Fragments arranged by size: 

                1209      78      57
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     402     434     667     699     790     822    1344 
   Size:        402      32     233      32      91      32     522

  Fragments arranged by size: 

                 522     402     233      91      32      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     220     576    1344 
   Size:        220     356     768

  Fragments arranged by size: 

                 768     356     220
 
ZraI GAC'GTC

Cuts at:      0    1152    1344 
   Size:       1152     192
 

 Enzymes that do cut:

    AatII     AclI     AcuI   AflIII     AhdI     AleI     AlfI     AloI
    AlwNI     ApaI    BamHI    BanII     BclI     BglI Bme1580I    BmgBI
     BpmI   Bpu10I    BpuEI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BsrBI
    BsrDI    BsrFI   BstAPI    BstXI    BstYI   Bsu36I     BtgI     BtsI
   DraIII     EaeI  EcoICRI  Eco57MI    EcoNI EcoO109I     FspI    Hin4I
   HincII     MscI     MslI   MspA1I     NaeI     NcoI   NgoMIV     NspI
     PasI    PflMI     PfoI     PpiI   PspOMI     PsrI     PstI    PvuII
     SacI    SexAI     SfcI     SmlI     SphI     StuI     StyI    TaqII
     TstI     XcmI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AfeI    AflII     AgeI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI     BanI     BbeI
     BbsI    BbvCI     BcgI    BciVI    BfrBI    BglII     BlpI     BmrI
     BmtI     BplI     BsaI    BsaBI    BsiWI    BspEI    BspHI    BspMI
    BsrGI   BssHII    BssSI    BstBI   BstEII  BstZ17I    BtgZI     ClaI
    CspCI     DraI     DrdI     EagI     EarI     EciI    EcoRI    EcoRV
     FalI     FseI    FspAI    HaeII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     NarI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PciI     PmeI     PmlI    PpuMI    PshAI     PsiI
    PspXI     PvuI    RsrII    SacII     SalI    SanDI     SapI     SbfI
     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SrfI     SspI     SwaI     TatI  Tth111I     XbaI     XhoI     XmaI
     XmnI
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