Restriction map for pF1KE0530
##################################
# NO CUT ENZYMES (  163 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AcsI        AcyI        AflIII      AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AvaII       AviII       
AvrII       BalI        BamHI       BbeI        BbrPI       
BbsI        BbvCI       BbvI        BcgI        BciVI       
BclI        BfaI        BglII       BlnI        BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BseRI       
BsgI        BsiEI       BsiWI       BsmBI       Bsp1407I    
BspDI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BsrI        BssHII      BssSI       
Bst1107I    BstBI       BstUI       BstXI       BstZ17I     
Bsu36I      CfoI        Cfr10I      ClaI        CpoI        
DraI        DraIII      DrdI        EaeI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoRI       EcoRV       FauI        
FbaI        Fnu4HI      FseI        FspI        HaeII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HpaI        ItaI        KasI        
KpnI        Ksp632I     KspI        MaeI        MamI        
MfeI        MluI        MluNI       MscI        MslI        
MspA1I      MunI        MvnI        NaeI        NarI        
NdeI        NgoMIV      NheI        NotI        NruI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        PvuII       RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SnaBI       SpeI        Sse8387I    SspBI       
SspI        StuI        SwaI        TaqI        TfiI        
TseI        Tsp509I     Tth111I     TthHB8I     XbaI        
XcmI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   63 enzymes) #
###################################

AccIII        416  TCCGGA               T^CCGGA
AciI          212  CCGC                 CCGC(-3/-1)
AfaI          309  GTAC                 GT^AC
AflII         229  CTTAAG               C^TTAAG
AlwI          297  GATCC                GGATC(4/5)
AlwNI         340  CAGGTTCTG            CAG[ACGT][ACGT][ACGT]^CTG
AspHI         205  GTGCTC               G[AT]GC[AT]^C
AvaI          361  CCCGGG               C^[CT]CG[GA]G
BanI          196  GGTGCC               G^G[CT][GA]CC
BanII           4  GGGCTC               G[GA]GC[CT]^C
BfrI          229  CTTAAG               C^TTAAG
BglI          382  GCCCAGGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI            6  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I        6  GCTCAGC              GC^T[ACGT]AGC
BsaWI         416  TCCGGA               [AT]^CCGG[AT]
BseAI         416  TCCGGA               T^CCGGA
BsiHKAI       205  GTGCTC               G[AT]GC[AT]^C
BsmAI         115  GAGAC                GTCTC(1/5)
BsmFI         365  GGGAC                GGGAC(10/14)
BsmI          328  GAATGC               GAATGC(1/-1)
BsoBI         361  CCCGGG               C^[CT]CG[GA]G
BspEI         416  TCCGGA               T^CCGGA
BspHI         246  TCATGA               T^CATGA
BstAPI         24  GCAGTTGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        106  GGTCACC              G^GT[ACGT]ACC
BstPI         106  GGTCACC              G^GT[ACGT]ACC
BstYI         296  AGATCC               [GA]^GATC[CT]
CelII           6  GCTCAGC              GC^T[ACGT]AGC
DpnII         297  GATC                 ^GATC
DraII         401  AGGCCCT              [GA]G^G[ACGT]CC[CT]
DsaI           72  CCATGG               C^C[GA][CT]GG
EcoO109I      401  AGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoO65I       106  GGTCACC              G^GT[ACGT]ACC
EcoT14I        72  CCATGG               C^C[AT][AT]GG
EcoT22I       330  ATGCAT               ATGCA^T
HinfI         117  GACTC                G^A[ACGT]TC
MaeII          38  ACGT                 A^CGT
MaeIII        107  GTCAC                ^GT[ACGT]AC
MboI          297  GATC                 ^GATC
MboII         234  GAAGA                GAAGA(8/7)
MflI          296  AGATCC               [GA]^GATC[CT]
MroI          416  TCCGGA               T^CCGGA
NcoI           72  CCATGG               C^CATGG
NdeII         297  GATC                 ^GATC
NsiI          330  ATGCAT               ATGCA^T
NspI          279  GCATGC               [GA]CATG^[CT]
PflMI          67  CCAGGCCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          117  GACTC                GAGTC(4/5)
PstI          346  CTGCAG               CTGCA^G
RcaI          246  TCATGA               T^CATGA
RsaI          309  GTAC                 GT^AC
Sau3AI        297  GATC                 ^GATC
SfcI          346  CTGCAG               C^T[GA][CT]AG
SmaI          361  CCCGGG               CCC^GGG
SmlI          229  CTTAAG               C^T[CT][GA]AG
SphI          279  GCATGC               GCATG^C
StyI           72  CCATGG               C^C[AT][AT]GG
Tsp45I        107  GTCAC                ^GT[GC]AC
TspRI         201  CACTG                CA[GC]TG[ACGT][ACGT]^
Van91I         67  CCAGGCCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         296  AGATCC               [GA]^GATC[CT]
XmaCI         361  CCCGGG               C^CCGGG
XmaI          361  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AluI          410  AGCT                 AG^CT
              446  AGCT                
BcnI          361  CCCGG                CC^[GC]GG
              362  CCGGG               
BmyI            4  GGGCTC               G[AGT]GC[ACT]^C
              205  GTGCTC              
BsiYI          67  CCAGGCCATGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
               72  CCATGGGCAGG         
BslI           67  CCAGGCCATGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
               72  CCATGGGCAGG         
Bsp1286I        4  GGGCTC               G[AGT]GC[ACT]^C
              205  GTGCTC              
Cac8I         279  GCATGC               GC[ACGT]^[ACGT]GC
              353  GCAAGC              
Cfr13I        389  GGGCC                G^G[ACGT]CC
              402  GGCCC               
DdeI            7  CTCAG                C^T[ACGT]AG
              176  CTGAG               
HapII         362  CCGG                 C^CGG
              417  CCGG                
HpaII         362  CCGG                 C^CGG
              417  CCGG                
HphI          108  TCACC                GGTGA(8/7)
              209  TCACC               
MspI          362  CCGG                 C^CGG
              417  CCGG                
NciI          361  CCCGG                CC^[GC]GG
              362  CCGGG               
NlaIV         196  GGTGCC               GG[ACGT]^[ACGT]CC
              388  GGGGCC              
Sau96I        389  GGGCC                G^G[ACGT]CC
              402  GGCCC               


#########################################
# 3 OR MORE CUT ENZYMES (   16 enzymes) #
#########################################

BpmI        BsaJI       BssKI       BstF5I      BstNI       
EcoRII      FokI        HaeIII      MnlI        MseI        
MvaI        MwoI        NlaIII      ScrFI       SfaNI       
Tru9I       
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