Restriction map for pF1KE0525
 (Linear) (Six-base) MAPSORT of: KIEE0525.seq  Check: 6057  from: 1  to: 1152



KIEE0525.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:15  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      56     856    1035    1152 
   Size:         56     800     179     117

  Fragments arranged by size: 

                 800     179     117      56
 
AleI CACnn'nnGTG

Cuts at:      0    1141    1152 
   Size:       1141      11
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     185    1152 
   Size:        185     967
 
AlwNI CAG_nnn'CTG

Cuts at:      0     449     459     964    1152 
   Size:        449      10     505     188

  Fragments arranged by size: 

                 505     449     188      10
 
ApaI G_GGCC'C

Cuts at:      0     437    1152 
   Size:        437     715
 
ApaLI G'TGCA_C

Cuts at:      0     214    1152 
   Size:        214     938
 
ApoI r'AATT_y

Cuts at:      0     409    1152 
   Size:        409     743
 
AvaI C'yCGr_G

Cuts at:      0     897     945    1152 
   Size:        897      48     207

  Fragments arranged by size: 

                 897     207      48
 
BanI G'GyrC_C

Cuts at:      0     707     868    1152 
   Size:        707     161     284

  Fragments arranged by size: 

                 707     284     161
 
BanII G_rGCy'C

Cuts at:      0     437    1106    1152 
   Size:        437     669      46

  Fragments arranged by size: 

                 669     437      46
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     155    1152 
   Size:        155     997
 
BglI GCCn_nnn'nGGC

Cuts at:      0     265    1152 
   Size:        265     887
 
BlpI GC'TnA_GC

Cuts at:      0    1019    1152 
   Size:       1019     133
 
Bme1580I G_kGCm'C

Cuts at:      0     218     437     871    1152 
   Size:        218     219     434     281

  Fragments arranged by size: 

                 434     281     219     218
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     912    1152 
   Size:        912     240
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      46     126    1152 
   Size:         46      80    1026

  Fragments arranged by size: 

                1026      80      46
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     282    1152 
   Size:        282     870
 
BsaAI yAC'GTr

Cuts at:      0     219    1152 
   Size:        219     933
 
BsaWI w'CCGG_w

Cuts at:      0     559    1152 
   Size:        559     593
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     202    1152 
   Size:        202     950
 
BsiHKAI G_wGCw'C

Cuts at:      0     218     786     878    1106    1152 
   Size:        218     568      92     228      46

  Fragments arranged by size: 

                 568     228     218      92      46
 
BsmI GAATG_Cn'

Cuts at:      0      37    1152 
   Size:         37    1115
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     192    1152 
   Size:        192     960
 
Bsp1286I G_dGCh'C

Cuts at:      0     218     437     786     871     878    1106    1152 
   Size:        218     219     349      85       7     228      46

  Fragments arranged by size: 

                 349     228     219     218      85      46       7
 
BspEI T'CCGG_A

Cuts at:      0     559    1152 
   Size:        559     593
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      56     856    1035    1070    1152 
   Size:         56     800     179      35      82

  Fragments arranged by size: 

                 800     179      82      56      35
 
BsrBI CCG'CTC

Cuts at:      0      54    1152 
   Size:         54    1098
 
BsrDI GCAATG_nn'

Cuts at:      0     329     827    1152 
   Size:        329     498     325

  Fragments arranged by size: 

                 498     329     325
 
BsrGI T'GTAC_A

Cuts at:      0     731    1152 
   Size:        731     421
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     829    1152 
   Size:        829     323
 
BstBI TT'CG_AA

Cuts at:      0     131    1152 
   Size:        131    1021
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     928    1152 
   Size:        928     224
 
BtgI C'CryG_G

Cuts at:      0     542    1152 
   Size:        542     610
 
BtsI GCAGTG_nn'

Cuts at:      0     837    1152 
   Size:        837     315
 
DraIII CAC_nnn'GTG

Cuts at:      0     657    1152 
   Size:        657     495
 
EaeI y'GGCC_r

Cuts at:      0     770    1152 
   Size:        770     382
 
EcoICRI GAG'CTC

Cuts at:      0    1104    1152 
   Size:       1104      48
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      46     126    1152 
   Size:         46      80    1026

  Fragments arranged by size: 

                1026      80      46
 
HaeII r_GCGC'y

Cuts at:      0      11    1152 
   Size:         11    1141
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0       4      36     323     355    1152 
   Size:          4      32     287      32     797

  Fragments arranged by size: 

                 797     287      32      32       4
 
HincII GTy'rAC

Cuts at:      0     336     623    1152 
   Size:        336     287     529

  Fragments arranged by size: 

                 529     336     287
 
HindIII A'AGCT_T

Cuts at:      0     106     125     394    1152 
   Size:        106      19     269     758

  Fragments arranged by size: 

                 758     269     106      19
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1148    1152 
   Size:       1148       4
 
MslI CAynn'nnrTG

Cuts at:      0     481     521    1141    1152 
   Size:        481      40     620      11

  Fragments arranged by size: 

                 620     481      40      11
 
MspA1I CmG'CkG

Cuts at:      0     184     195    1152 
   Size:        184      11     957

  Fragments arranged by size: 

                 957     184      11
 
PasI CC'CwG_GG

Cuts at:      0     119    1152 
   Size:        119    1033
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     477    1152 
   Size:        477     675
 
PfoI T'CCnGG_A

Cuts at:      0     350     497    1152 
   Size:        350     147     655

  Fragments arranged by size: 

                 655     350     147
 
PmlI CAC'GTG

Cuts at:      0     219    1152 
   Size:        219     933
 
PspOMI G'GGCC_C

Cuts at:      0     433    1152 
   Size:        433     719
 
PspXI vC'TCGA_Gb

Cuts at:      0     945    1152 
   Size:        945     207
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     682     714    1152 
   Size:        682      32     438

  Fragments arranged by size: 

                 682     438      32
 
PstI C_TGCA'G

Cuts at:      0     164    1152 
   Size:        164     988
 
PvuII CAG'CTG

Cuts at:      0     195    1152 
   Size:        195     957
 
SacI G_AGCT'C

Cuts at:      0    1106    1152 
   Size:       1106      46
 
SfcI C'TryA_G

Cuts at:      0     160    1128    1152 
   Size:        160     968      24

  Fragments arranged by size: 

                 968     160      24
 
SmlI C'TyrA_G

Cuts at:      0     945    1152 
   Size:        945     207
 
StyI C'CwwG_G

Cuts at:      0     400    1152 
   Size:        400     752
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     595    1152 
   Size:        595     557
 
TatI w'GTAC_w

Cuts at:      0     731    1152 
   Size:        731     421
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     514     546    1152 
   Size:        514      32     606

  Fragments arranged by size: 

                 606     514      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     861    1152 
   Size:        861     291
 
XhoI C'TCGA_G

Cuts at:      0     945    1152 
   Size:        945     207
 

 Enzymes that do cut:

     AarI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AvaI
     BanI    BanII     BbsI     BglI     BlpI Bme1580I     BplI     BpmI
     BsaI    BsaAI    BsaWI    BseRI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspEI    BspMI    BsrBI    BsrDI    BsrGI   BstAPI    BstBI    BstXI
     BtgI     BtsI   DraIII     EaeI  EcoICRI  Eco57MI    HaeII    Hin4I
   HincII  HindIII     MmeI     MslI   MspA1I     PasI    PflMI     PfoI
     PmlI   PspOMI    PspXI     PsrI     PstI    PvuII     SacI     SfcI
     SmlI     StyI    TaqII     TatI     TstI     XcmI     XhoI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AloI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII
    BmgBI     BmrI     BmtI   Bpu10I    BpuEI    BsaBI    BsaHI    BsaXI
    BseYI     BsgI    BsiEI    BsiWI    BspHI    BsrFI   BssHII    BssSI
   BstEII    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI
     DrdI     EagI     EarI     EciI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI     HpaI     KasI     KpnI     MfeI
     MluI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PciI     PmeI     PpiI
    PpuMI    PshAI     PsiI     PvuI    RsrII    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
  Tth111I     XbaI     XmaI     XmnI     ZraI
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