Restriction map for pF1KE0468
##################################
# NO CUT ENZYMES (  116 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AflII       
AgeI        AocI        Aor51HI     AscI        AseI        
AsnI        Asp700I     Asp718I     AspI        AviII       
AvrII       BbrPI       BcgI        BciVI       BfrI        
BglI        BlnI        BsaAI       BsaBI       BsaWI       
BseAI       BsgI        BsiEI       BsiWI       BsmBI       
Bsp1407I    BspDI       BspEI       BspHI       BsrBI       
BsrDI       BsrGI       BssHII      BssSI       Bst1107I    
BstBI       BstF5I      BstZ17I     Bsu36I      ClaI        
CpoI        DraI        DraIII      EagI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoRI       
EcoRV       EcoT22I     FokI        FseI        FspI        
HgaI        HincII      HindII      HindIII     HpaI        
KpnI        KspI        MamI        MfeI        MluI        
MroI        MunI        NcoI        NdeI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SbfI        ScaI        SexAI       SfiI        
SfuI        SgrAI       SnaBI       SpeI        Sse8387I    
SspBI       SspI        StuI        SwaI        TaqI        
Tth111I     TthHB8I     Van91I      XbaI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AatII         603  GACGTC               GACGT^C
AccII        1110  CGCG                 CG^CG
AcsI          281  AAATTC               [GA]^AATT[CT]
AflIII        224  ACATGT               A^C[GA][CT]GT
AhdI           68  GACCTGCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        211  GTGCAC               G^TGCAC
AlwNI          65  CAGGACCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI         211  GTGCAC               G^TGCAC
ApoI          281  AAATTC               [GA]^AATT[CT]
AspEI          68  GACCTGCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI         211  GTGCAC               G[AT]GC[AT]^C
BalI           17  TGGCCA               TGG^CCA
BamHI          32  GGATCC               G^GATCC
BbsI          673  GTCTTC               GAAGAC(2/6)
BbvCI        1019  CCTCAGC              CCTCAGC(-5/-2)
BclI          314  TGATCA               T^GATCA
BglII         386  AGATCT               A^GATCT
BlpI          845  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      845  GCTCAGC              GC^T[ACGT]AGC
BpuAI         673  GTCTTC               GAAGAC(2/6)
BsiHKAI       211  GTGCAC               G[AT]GC[AT]^C
BsmI           56  GCATTC               GAATGC(1/-1)
BspLU11I      224  ACATGT               A^CATGT
BspMI          69  ACCTGC               ACCTGC(4/8)
BsrFI          52  GCCGGC               [GA]^CCGG[CT]
BstAPI        418  GCAAAGAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI        1110  CGCG                 CG^CG
BstXI         404  CCAACAAGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII         845  GCTCAGC              GC^T[ACGT]AGC
Cfr10I         52  GCCGGC               [GA]^CCGG[CT]
DrdI          988  GACAGCCAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI           17  TGGCCA               [CT]^GGCC[GA]
Eam1105I       68  GACCTGCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          114  GAAGAG               CTCTTC(1/4)
FbaI          314  TGATCA               T^GATCA
Ksp632I       114  GAAGAG               CTCTTC(1/4)
MluNI          17  TGGCCA               TGG^CCA
MscI           17  TGGCCA               TGG^CCA
MslI          525  CATCAACATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        962  CAGCTG               C[AC]G^C[GT]G
MvnI         1110  CGCG                 CG^CG
NaeI           52  GCCGGC               GCC^GGC
NgoMIV         52  GCCGGC               G^CCGGC
PvuII         962  CAGCTG               CAG^CTG
SapI          114  GAAGAGC              GCTCTTC(1/4)
SfaNI         941  GCATC                GCATC(5/9)
SmaI         1064  CCCGGG               CCC^GGG
TfiI          108  GATTC                G^A[AT]TC
XcmI          434  CCACTGCAGCCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI        1064  CCCGGG               C^CCGGG
XmaI         1064  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AfaI          250  GTAC                 GT^AC
              519  GTAC                
ApaI          789  GGGCCC               GGGCC^C
             1060  GGGCCC              
AvaI          976  CCCGAG               C^[CT]CG[GA]G
             1064  CCCGGG              
BanII         789  GGGCCC               G[GA]GC[CT]^C
             1060  GGGCCC              
BfaI          263  CTAG                 C^TAG
              781  CTAG                
BseRI         457  GAGGAG               GAGGAG(10/8)
              460  GAGGAG              
BsmFI         722  GGGAC                GGGAC(10/14)
              806  GTCCC               
BsoBI         976  CCCGAG               C^[CT]CG[GA]G
             1064  CCCGGG              
BstEII        996  GGTCACC              G^GT[ACGT]ACC
             1035  GGTCACC             
BstPI         996  GGTCACC              G^GT[ACGT]ACC
             1035  GGTCACC             
DsaI          167  CCGTGG               C^C[GA][CT]GG
              288  CCGTGG              
EcoO65I       996  GGTCACC              G^GT[ACGT]ACC
             1035  GGTCACC             
MaeI          263  CTAG                 C^TAG
              781  CTAG                
MaeII         521  ACGT                 A^CGT
              604  ACGT                
MseI           44  TTAA                 T^TAA
             1043  TTAA                
PstI          255  CTGCAG               CTGCA^G
              437  CTGCAG              
RsaI          250  GTAC                 GT^AC
              519  GTAC                
SfcI          255  CTGCAG               C^T[GA][CT]AG
              437  CTGCAG              
SmlI          193  CTCAAG               C^T[CT][GA]AG
              907  CTTGAG              
SphI          119  GCATGC               GCATG^C
              198  GCATGC              
Tru9I          44  TTAA                 T^TAA
             1043  TTAA                
Tsp509I        79  AATT                 ^AATT
              282  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   69 enzymes) #
#########################################

AciI        AcyI        AluI        AlwI        AvaII       
BanI        BbeI        BbvI        BcnI        BmyI        
BpmI        BsaHI       BsaI        BsaJI       BsiYI       
BslI        BsmAI       Bsp1286I    BsrI        BssKI       
BstNI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      HaeII       HaeIII      
HapII       HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        KasI        MaeIII      
MboI        MboII       MflI        MnlI        MspI        
MvaI        MwoI        NarI        NciI        NdeII       
NlaIII      NlaIV       NspI        PleI        PpuMI       
Sau3AI      Sau96I      ScrFI       SfoI        StyI        
TseI        Tsp45I      TspRI       XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com