Restriction map for pF1KE0468
 (Linear) (Six-base) MAPSORT of: KIEE0468.seq  Check: 1226  from: 1  to: 1155



KIEE0468.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:14  ..

AatII G_ACGT'C

Cuts at:      0     607    1155 
   Size:        607     548
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     660     793    1155 
   Size:        660     133     362

  Fragments arranged by size: 

                 660     362     133
 
AflIII A'CryG_T

Cuts at:      0     224    1155 
   Size:        224     931
 
AhdI GACnn_n'nnGTC

Cuts at:      0      73    1155 
   Size:         73    1082
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     599    1155 
   Size:        599     556
 
AlwNI CAG_nnn'CTG

Cuts at:      0      70    1155 
   Size:         70    1085
 
ApaI G_GGCC'C

Cuts at:      0     793    1064    1155 
   Size:        793     271      91

  Fragments arranged by size: 

                 793     271      91
 
ApaLI G'TGCA_C

Cuts at:      0     211    1155 
   Size:        211     944
 
ApoI r'AATT_y

Cuts at:      0     281    1155 
   Size:        281     874
 
AvaI C'yCGr_G

Cuts at:      0     976    1064    1155 
   Size:        976      88      91

  Fragments arranged by size: 

                 976      91      88
 
BamHI G'GATC_C

Cuts at:      0      32    1155 
   Size:         32    1123
 
BanI G'GyrC_C

Cuts at:      0     292     362     488     541     625     878    1093    1155 
   Size:        292      70     126      53      84     253     215      62

  Fragments arranged by size: 

                 292     253     215     126      84      70      62      53
            
 
BanII G_rGCy'C

Cuts at:      0     793    1064    1155 
   Size:        793     271      91

  Fragments arranged by size: 

                 793     271      91
 
BbeI G_GCGC'C

Cuts at:      0     366     492     629     882    1155 
   Size:        366     126     137     253     273

  Fragments arranged by size: 

                 366     273     253     137     126
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     666    1155 
   Size:        666     489
 
BbvCI CC'TCA_GC

Cuts at:      0    1020    1155 
   Size:       1020     135
 
BclI T'GATC_A

Cuts at:      0     314    1155 
   Size:        314     841
 
BglII A'GATC_T

Cuts at:      0     386    1155 
   Size:        386     769
 
BlpI GC'TnA_GC

Cuts at:      0     846    1155 
   Size:        846     309
 
Bme1580I G_kGCm'C

Cuts at:      0     215     793     977    1064    1098    1117    1155 
   Size:        215     578     184      87      34      19      38

  Fragments arranged by size: 

                 578     215     184      87      38      34      19
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     964    1147    1155 
   Size:        964     183       8

  Fragments arranged by size: 

                 964     183       8
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      47     589    1062    1155 
   Size:         47     542     473      93

  Fragments arranged by size: 

                 542     473      93      47
 
Bpu10I CC'TnA_GC

Cuts at:      0    1020    1155 
   Size:       1020     135
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     178     928    1155 
   Size:        178     750     227

  Fragments arranged by size: 

                 750     227     178
 
BsaI GGTCTCn'nnnn_

Cuts at:      0       9     598     625    1155 
   Size:          9     589      27     530

  Fragments arranged by size: 

                 589     530      27       9
 
BsaHI Gr'CG_yC

Cuts at:      0     363     489     604     626     879    1155 
   Size:        363     126     115      22     253     276

  Fragments arranged by size: 

                 363     276     253     126     115      22
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     502     532     913     943    1155 
   Size:        502      30     381      30     212

  Fragments arranged by size: 

                 502     381     212      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     472     475    1155 
   Size:        472       3     680

  Fragments arranged by size: 

                 680     472       3
 
BseYI C'CCAG_C

Cuts at:      0     156     346     792     809    1155 
   Size:        156     190     446      17     346

  Fragments arranged by size: 

                 446     346     190     156      17
 
BsiHKAI G_wGCw'C

Cuts at:      0     215    1155 
   Size:        215     940
 
BsmI GAATG_Cn'

Cuts at:      0      56    1155 
   Size:         56    1099
 
Bsp1286I G_dGCh'C

Cuts at:      0     215     793     977    1064    1098    1117    1155 
   Size:        215     578     184      87      34      19      38

  Fragments arranged by size: 

                 578     215     184      87      38      34      19
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      78    1155 
   Size:         78    1077
 
BsrFI r'CCGG_y

Cuts at:      0      52    1155 
   Size:         52    1103
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     424    1155 
   Size:        424     731
 
BstEII G'GTnAC_C

Cuts at:      0     996    1035    1155 
   Size:        996      39     120

  Fragments arranged by size: 

                 996     120      39
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     411    1155 
   Size:        411     744
 
BstYI r'GATC_y

Cuts at:      0      32     129     386     563     711    1155 
   Size:         32      97     257     177     148     444

  Fragments arranged by size: 

                 444     257     177     148      97      32
 
BtgI C'CryG_G

Cuts at:      0     167     288    1155 
   Size:        167     121     867

  Fragments arranged by size: 

                 867     167     121
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     168    1155 
   Size:        168     987
 
BtsI GCAGTG_nn'

Cuts at:      0     434     497     871    1155 
   Size:        434      63     374     284

  Fragments arranged by size: 

                 434     374     284      63
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     994    1155 
   Size:        994     161
 
EaeI y'GGCC_r

Cuts at:      0      17    1155 
   Size:         17    1138
 
EarI CTCTTCn'nnn_

Cuts at:      0     109    1155 
   Size:        109    1046
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      47     589     660     793    1062    1155 
   Size:         47     542      71     133     269      93

  Fragments arranged by size: 

                 542     269     133      93      71      47
 
EcoO109I rG'GnC_Cy

Cuts at:      0      67     768     789    1060    1061    1086    1148    1155 
   Size:         67     701      21     271       1      25      62       7

  Fragments arranged by size: 

                 701     271      67      62      25      21       7       1
            
 
HaeII r_GCGC'y

Cuts at:      0     366     492     629     882     889    1155 
   Size:        366     126     137     253       7     266

  Fragments arranged by size: 

                 366     266     253     137     126       7
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      59      91     913     945    1155 
   Size:         59      32     822      32     210

  Fragments arranged by size: 

                 822     210      59      32      32
 
KasI G'GCGC_C

Cuts at:      0     362     488     625     878    1155 
   Size:        362     126     137     253     277

  Fragments arranged by size: 

                 362     277     253     137     126
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     428    1155 
   Size:        428     727
 
MscI TGG'CCA

Cuts at:      0      19    1155 
   Size:         19    1136
 
MslI CAynn'nnrTG

Cuts at:      0     529    1155 
   Size:        529     626
 
MspA1I CmG'CkG

Cuts at:      0     964    1155 
   Size:        964     191
 
NaeI GCC'GGC

Cuts at:      0      54    1155 
   Size:         54    1101
 
NarI GG'CG_CC

Cuts at:      0     363     489     626     879    1155 
   Size:        363     126     137     253     276

  Fragments arranged by size: 

                 363     276     253     137     126
 
NgoMIV G'CCGG_C

Cuts at:      0      52    1155 
   Size:         52    1103
 
NspI r_CATG'y

Cuts at:      0     123     202     228     534    1155 
   Size:        123      79      26     306     621

  Fragments arranged by size: 

                 621     306     123      79      26
 
PciI A'CATG_T

Cuts at:      0     224    1155 
   Size:        224     931
 
PfoI T'CCnGG_A

Cuts at:      0      63     566    1155 
   Size:         63     503     589

  Fragments arranged by size: 

                 589     503      63
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     322     354    1155 
   Size:        322      32     801

  Fragments arranged by size: 

                 801     322      32
 
PpuMI rG'GwC_Cy

Cuts at:      0      67     768    1148    1155 
   Size:         67     701     380       7

  Fragments arranged by size: 

                 701     380      67       7
 
PspOMI G'GGCC_C

Cuts at:      0     789    1060    1155 
   Size:        789     271      95

  Fragments arranged by size: 

                 789     271      95
 
PstI C_TGCA'G

Cuts at:      0     259     441    1155 
   Size:        259     182     714

  Fragments arranged by size: 

                 714     259     182
 
PvuII CAG'CTG

Cuts at:      0     964    1155 
   Size:        964     191
 
SapI GCTCTTCn'nnn_

Cuts at:      0     109    1155 
   Size:        109    1046
 
SfcI C'TryA_G

Cuts at:      0     255     437    1155 
   Size:        255     182     718

  Fragments arranged by size: 

                 718     255     182
 
SfoI GGC'GCC

Cuts at:      0     364     490     627     880    1155 
   Size:        364     126     137     253     275

  Fragments arranged by size: 

                 364     275     253     137     126
 
SmaI CCC'GGG

Cuts at:      0    1066    1155 
   Size:       1066      89
 
SmlI C'TyrA_G

Cuts at:      0     193     907    1155 
   Size:        193     714     248

  Fragments arranged by size: 

                 714     248     193
 
SphI G_CATG'C

Cuts at:      0     123     202    1155 
   Size:        123      79     953

  Fragments arranged by size: 

                 953     123      79
 
StyI C'CwwG_G

Cuts at:      0     428     635     797     833    1089    1155 
   Size:        428     207     162      36     256      66

  Fragments arranged by size: 

                 428     256     207     162      66      36
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     163     360     381    1155 
   Size:        163     197      21     774

  Fragments arranged by size: 

                 774     197     163      21
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     441    1155 
   Size:        441     714
 
XmaI C'CCGG_G

Cuts at:      0    1064    1155 
   Size:       1064      91
 
ZraI GAC'GTC

Cuts at:      0     605    1155 
   Size:        605     550
 

 Enzymes that do cut:

    AatII     AcuI   AflIII     AhdI     AlfI    AlwNI     ApaI    ApaLI
     ApoI     AvaI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI
     BclI    BglII     BlpI Bme1580I     BmrI     BpmI   Bpu10I    BpuEI
     BsaI    BsaHI    BsaXI    BseRI    BseYI  BsiHKAI     BsmI Bsp1286I
    BspMI    BsrFI   BstAPI   BstEII    BstXI    BstYI     BtgI    BtgZI
     BtsI     DrdI     EaeI     EarI  Eco57MI EcoO109I    HaeII    Hin4I
     KasI     MmeI     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV
     NspI     PciI     PfoI     PpiI    PpuMI   PspOMI     PstI    PvuII
     SapI     SfcI     SfoI     SmaI     SmlI     SphI     StyI    TaqII
     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AleI
     AloI     AscI     AseI    AsiSI    AvrII     BaeI     BcgI    BciVI
    BfrBI     BglI    BmgBI     BmtI     BplI    BsaAI    BsaBI    BsaWI
     BsgI    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BsrBI    BsrDI
    BsrGI   BssHII    BssSI    BstBI  BstZ17I   Bsu36I     ClaI    CspCI
     DraI   DraIII     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI     KpnI
     MfeI     MluI     NcoI     NdeI     NheI     NotI     NruI     NsiI
     PacI     PasI    PflMI     PmeI     PmlI    PshAI     PsiI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SbfI
     ScaI    SexAI     SfiI     SgfI    SgrAI    SnaBI     SpeI     SrfI
     SspI     StuI     SwaI     TatI     TstI  Tth111I     XbaI     XhoI
     XmnI
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