Restriction map for pF1KE0465
 (Linear) (Six-base) MAPSORT of: KIEE0465.seq  Check: 1623  from: 1  to: 657



KIEE0465.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:14  ..

AleI CACnn'nnGTG

Cuts at:      0     269     657 
   Size:        269     388
 
AlwNI CAG_nnn'CTG

Cuts at:      0     361     657 
   Size:        361     296
 
ApaI G_GGCC'C

Cuts at:      0     456     657 
   Size:        456     201
 
ApoI r'AATT_y

Cuts at:      0     135     657 
   Size:        135     522
 
AvrII C'CTAG_G

Cuts at:      0     316     657 
   Size:        316     341
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     205     238     657 
   Size:        205      33     419

  Fragments arranged by size: 

                 419     205      33
 
BanI G'GyrC_C

Cuts at:      0     483     513     543     657 
   Size:        483      30      30     114

  Fragments arranged by size: 

                 483     114      30      30
 
BanII G_rGCy'C

Cuts at:      0     456     657 
   Size:        456     201
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     233     657 
   Size:        233     424
 
BlpI GC'TnA_GC

Cuts at:      0     392     437     467     497     527     587     657 
   Size:        392      45      30      30      30      60      70

  Fragments arranged by size: 

                 392      70      60      45      30      30      30
 
Bme1580I G_kGCm'C

Cuts at:      0     456     657 
   Size:        456     201
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     157     173     657 
   Size:        157      16     484

  Fragments arranged by size: 

                 484     157      16
 
Bpu10I CC'TnA_GC

Cuts at:      0     282     657 
   Size:        282     375
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     137     296     657 
   Size:        137     159     361

  Fragments arranged by size: 

                 361     159     137
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     392     657 
   Size:        392     265
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      45     657 
   Size:         45     612
 
Bsp1286I G_dGCh'C

Cuts at:      0     456     657 
   Size:        456     201
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     373     520     657 
   Size:        373     147     137

  Fragments arranged by size: 

                 373     147     137
 
BstEII G'GTnAC_C

Cuts at:      0      91     212     657 
   Size:         91     121     445

  Fragments arranged by size: 

                 445     121      91
 
BtgI C'CryG_G

Cuts at:      0     270     657 
   Size:        270     387
 
EarI CTCTTCn'nnn_

Cuts at:      0      43     657 
   Size:         43     614
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     446     657 
   Size:        446     211
 
EcoO109I rG'GnC_Cy

Cuts at:      0     452     453     657 
   Size:        452       1     204

  Fragments arranged by size: 

                 452     204       1
 
HindIII A'AGCT_T

Cuts at:      0       4     657 
   Size:          4     653
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     471     531     657 
   Size:        471      60     126

  Fragments arranged by size: 

                 471     126      60
 
MslI CAynn'nnrTG

Cuts at:      0     269     657 
   Size:        269     388
 
MspA1I CmG'CkG

Cuts at:      0     361     373     391     406     436     466     526     565 
   Size:        361      12      18      15      30      30      60      39

Cuts at:    565     586     657 
   Size:         21      71

  Fragments arranged by size: 

                 361      71      60      39      30      30      21      18
                  15      12
 
PasI CC'CwG_GG

Cuts at:      0     187     657 
   Size:        187     470
 
PfoI T'CCnGG_A

Cuts at:      0     243     657 
   Size:        243     414
 
PshAI GACnn'nnGTC

Cuts at:      0      89     657 
   Size:         89     568
 
PspOMI G'GGCC_C

Cuts at:      0     452     657 
   Size:        452     205
 
PstI C_TGCA'G

Cuts at:      0     366     405     450     480     510     540     570     657 
   Size:        366      39      45      30      30      30      30      87

  Fragments arranged by size: 

                 366      87      45      39      30      30      30      30
            
 
PvuII CAG'CTG

Cuts at:      0     361     391     406     436     466     526     565     586 
   Size:        361      30      15      30      30      60      39      21

Cuts at:    586     657 
   Size:         71

  Fragments arranged by size: 

                 361      71      60      39      30      30      30      21
                  15
 
SfcI C'TryA_G

Cuts at:      0     362     401     428     446     476     506     536     566 
   Size:        362      39      27      18      30      30      30      30

Cuts at:    566     596     657 
   Size:         30      61

  Fragments arranged by size: 

                 362      61      39      30      30      30      30      30
                  27      18
 
SspI AAT'ATT

Cuts at:      0     117     657 
   Size:        117     540
 
StyI C'CwwG_G

Cuts at:      0     316     657 
   Size:        316     341
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     110     657 
   Size:        110     547
 

 Enzymes that do cut:

     AleI    AlwNI     ApaI     ApoI    AvrII     BaeI     BanI    BanII
    BciVI     BlpI Bme1580I     BmrI   Bpu10I    BseRI     BsgI    BsmBI
 Bsp1286I   BstAPI   BstEII     BtgI     EarI    EcoNI EcoO109I  HindIII
     MmeI     MslI   MspA1I     PasI     PfoI    PshAI   PspOMI     PstI
    PvuII     SfcI     SspI     StyI    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AlfI     AloI    ApaLI     AscI     AseI
    AsiSI     AvaI    BamHI     BbeI     BbsI    BbvCI     BcgI     BclI
    BfrBI     BglI    BglII    BmgBI     BmtI     BplI     BpmI    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI    BsiEI
  BsiHKAI    BsiWI     BsmI    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI    BstBI    BstXI    BstYI  BstZ17I
   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EciI  EcoICRI  Eco57MI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII     HpaI     KasI
     KpnI     MfeI     MluI     MscI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PciI
    PflMI     PmeI     PmlI     PpiI    PpuMI     PsiI    PspXI     PsrI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     StuI     SwaI     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
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