Restriction map for pF1KE0463
##################################
# NO CUT ENZYMES (  148 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AviII       AvrII       BalI        BamHI       
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BclI        BfrI        BlnI        BlpI        BpmI        
Bpu1102I    BsaBI       BsaHI       BsaWI       BseAI       
BseRI       BsgI        BsiWI       BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrDI       BsrGI       BssHII      Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstXI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
DraI        DrdI        DsaI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoO65I     EcoRI       EcoRV       EcoT14I     EcoT22I     
FauI        FbaI        FseI        FspI        HaeII       
Hin1I       HincII      HindII      HindIII     HpaI        
HphI        KasI        KpnI        KspI        MamI        
MluI        MluNI       MroI        MscI        MspA1I      
NarI        NcoI        NdeI        NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsrII       SacII       
SalI        SbfI        SexAI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
StyI        SwaI        Tth111I     Van91I      XbaI        
XcmI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AccII        1214  CGCG                 CG^CG
AcsI         1159  AAATTC               [GA]^AATT[CT]
AflIII        633  ACACGT               A^C[GA][CT]GT
AlwI          825  GGATC                GGATC(4/5)
ApoI         1159  AAATTC               [GA]^AATT[CT]
AvaI         1094  CCCGGG               C^[CT]CG[GA]G
BanI         1108  GGCACC               G^G[CT][GA]CC
BbsI          500  GAAGAC               GAAGAC(2/6)
BglI         1174  GCCTCAGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         519  AGATCT               A^GATCT
BpuAI         500  GAAGAC               GAAGAC(2/6)
BsaAI         634  CACGTA               [CT]AC^GT[GA]
BsaI         1038  GGTCTC               GGTCTC(1/5)
BsiEI        1113  CGACCG               CG[GA][CT]^CG
BsmAI        1039  GTCTC                GTCTC(1/5)
BsoBI        1094  CCCGGG               C^[CT]CG[GA]G
BsrBI        1218  CCGCTC               CCGCTC(-3/-3)
BsrFI        1203  GCCGGC               [GA]^CCGG[CT]
BssSI        1120  CACGAG               CACGAG(-5/-1)
BstUI        1214  CGCG                 CG^CG
BstYI         519  AGATCT               [GA]^GATC[CT]
CfoI         1215  GCGC                 GCG^C
Cfr10I       1203  GCCGGC               [GA]^CCGG[CT]
DraII        1097  GGGTCCC              [GA]G^G[ACGT]CC[CT]
DraIII        970  CACAATGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoNI         336  CCTGGAAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I     1097  GGGTCCC              [GA]G^G[ACGT]CC[CT]
HaeIII       1182  GGCC                 GG^CC
HgaI         1212  GACGC                GACGC(5/10)
HhaI         1215  GCGC                 GCG^C
HinP1I       1215  GCGC                 G^CGC
MfeI          772  CAATTG               C^AATTG
MflI          519  AGATCT               [GA]^GATC[CT]
MunI          772  CAATTG               C^AATTG
MvnI         1214  CGCG                 CG^CG
NaeI         1203  GCCGGC               GCC^GGC
NgoMIV       1203  GCCGGC               G^CCGGC
NspI          807  ACATGC               [GA]CATG^[CT]
PpuMI        1097  GGGTCCC              [GA]G^G[AT]CC[CT]
ScaI          585  AGTACT               AGT^ACT
SmaI         1094  CCCGGG               CCC^GGG
SmlI           12  CTTGAG               C^T[CT][GA]AG
TaqI         1005  TCGA                 T^CGA
TthHB8I      1005  TCGA                 T^CGA
XhoII         519  AGATCT               [GA]^GATC[CT]
XmaCI        1094  CCCGGG               C^CCGGG
XmaI         1094  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AfaI          586  GTAC                 GT^AC
              637  GTAC                
AlwNI         442  CAGAAACTG            CAG[ACGT][ACGT][ACGT]^CTG
              959  CAGACTCTG           
AspHI          15  GAGCTC               G[AT]GC[AT]^C
              331  GAGCTC              
AvaII          70  GGTCC                G^G[AT]CC
             1098  GGTCC               
BanII          15  GAGCTC               G[GA]GC[CT]^C
              331  GAGCTC              
BbvI          844  GCTGC                GCAGC(8/12)
              847  GCTGC               
BmyI           15  GAGCTC               G[AGT]GC[ACT]^C
              331  GAGCTC              
BsiHKAI        15  GAGCTC               G[AT]GC[AT]^C
              331  GAGCTC              
BsiYI         336  CCTGGAAAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             1197  CCTGAAGCCGG         
BslI          336  CCTGGAAAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             1197  CCTGAAGCCGG         
BsmFI         210  GGGAC                GGGAC(10/14)
             1099  GTCCC               
Bsp1286I       15  GAGCTC               G[AGT]GC[ACT]^C
              331  GAGCTC              
BsrI          232  ACTGG                ACTGG(1/-1)
              373  CCAGT               
Cfr13I         70  GGTCC                G^G[ACGT]CC
             1098  GGTCC               
EarI         1137  GAAGAG               CTCTTC(1/4)
             1240  GAAGAG              
Ksp632I      1137  GAAGAG               CTCTTC(1/4)
             1240  GAAGAG              
MaeII         635  ACGT                 A^CGT
              720  ACGT                
PleI          961  GACTC                GAGTC(4/5)
             1072  GAGTC               
RsaI          586  GTAC                 GT^AC
              637  GTAC                
SacI           15  GAGCTC               GAGCT^C
              331  GAGCTC              
SapI         1137  GAAGAGC              GCTCTTC(1/4)
             1240  GAAGAGC             
Sau96I         70  GGTCC                G^G[ACGT]CC
             1098  GGTCC               
TfiI          909  GATTC                G^A[AT]TC
             1002  GATTC               
TseI          844  GCTGC                G^C[AT]GC
              847  GCTGC               
Tsp45I       1118  GTCAC                ^GT[GC]AC
             1210  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   38 enzymes) #
#########################################

AciI        AluI        BcnI        BfaI        BsaJI       
BssKI       BstF5I      BstNI       Cac8I       DdeI        
DpnII       EcoRII      Fnu4HI      FokI        HapII       
HinfI       HpaII       ItaI        MaeI        MaeIII      
MboI        MboII       MnlI        MseI        MslI        
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       Sau3AI      ScrFI       SfaNI       
Tru9I       Tsp509I     TspRI       
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