Restriction map for pF1KE0458
 (Linear) (Six-base) MAPSORT of: KIEE0458.seq  Check: 2503  from: 1  to: 678



KIEE0458.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:14  ..

AfeI AGC'GCT

Cuts at:      0     281     678 
   Size:        281     397
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      28      60     124     156     458     490     678 
   Size:         28      32      64      32     302      32     188

  Fragments arranged by size: 

                 302     188      64      32      32      32      28
 
AvaI C'yCGr_G

Cuts at:      0     148     678 
   Size:        148     530
 
BanI G'GyrC_C

Cuts at:      0     520     678 
   Size:        520     158
 
BanII G_rGCy'C

Cuts at:      0      65     678 
   Size:         65     613
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     408     678 
   Size:        408     270
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     180     214     229     263     678 
   Size:        180      34      15      34     415

  Fragments arranged by size: 

                 415     180      34      34      15
 
BlpI GC'TnA_GC

Cuts at:      0     614     678 
   Size:        614      64
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     253     678 
   Size:        253     425
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     386     625     678 
   Size:        386     239      53

  Fragments arranged by size: 

                 386     239      53
 
Bpu10I CC'TnA_GC

Cuts at:      0     181     678 
   Size:        181     497
 
BseYI C'CCAG_C

Cuts at:      0     441     486     590     678 
   Size:        441      45     104      88

  Fragments arranged by size: 

                 441     104      88      45
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     528     678 
   Size:        528     150
 
BsiHKAI G_wGCw'C

Cuts at:      0     557     678 
   Size:        557     121
 
Bsp1286I G_dGCh'C

Cuts at:      0      65     557     678 
   Size:         65     492     121

  Fragments arranged by size: 

                 492     121      65
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     320     678 
   Size:        320     358
 
BssHII G'CGCG_C

Cuts at:      0     160     678 
   Size:        160     518
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     585     678 
   Size:        585      93
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     531     678 
   Size:        531     147
 
BstYI r'GATC_y

Cuts at:      0      18     269     332     678 
   Size:         18     251      63     346

  Fragments arranged by size: 

                 346     251      63      18
 
BtgI C'CryG_G

Cuts at:      0      49     516     678 
   Size:         49     467     162

  Fragments arranged by size: 

                 467     162      49
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     412     678 
   Size:        412     266
 
EarI CTCTTCn'nnn_

Cuts at:      0      82     678 
   Size:         82     596
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      19     678 
   Size:         19     659
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     386     625     678 
   Size:        386     239      53

  Fragments arranged by size: 

                 386     239      53
 
EcoO109I rG'GnC_Cy

Cuts at:      0     140     491     512     678 
   Size:        140     351      21     166

  Fragments arranged by size: 

                 351     166     140      21
 
EcoRV GAT'ATC

Cuts at:      0     453     678 
   Size:        453     225
 
HaeII r_GCGC'y

Cuts at:      0     283     678 
   Size:        283     395
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      10      22      42      54      64      96     393     425 
   Size:         10      12      20      12      10      32     297      32

Cuts at:    425     678 
   Size:        253

  Fragments arranged by size: 

                 297     253      32      32      20      12      12      10
                  10
 
HincII GTy'rAC

Cuts at:      0     639     678 
   Size:        639      39
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      14     522     624     678 
   Size:         14     508     102      54

  Fragments arranged by size: 

                 508     102      54      14
 
MspA1I CmG'CkG

Cuts at:      0      51     364     618     678 
   Size:         51     313     254      60

  Fragments arranged by size: 

                 313     254      60      51
 
NcoI C'CATG_G

Cuts at:      0     516     678 
   Size:        516     162
 
NspI r_CATG'y

Cuts at:      0     115     678 
   Size:        115     563
 
PpuMI rG'GwC_Cy

Cuts at:      0     140     491     678 
   Size:        140     351     187

  Fragments arranged by size: 

                 351     187     140
 
PstI C_TGCA'G

Cuts at:      0     287     678 
   Size:        287     391
 
PvuII CAG'CTG

Cuts at:      0     364     678 
   Size:        364     314
 
SacII CC_GC'GG

Cuts at:      0      52     678 
   Size:         52     626
 
SanDI GG'GwC_CC

Cuts at:      0     140     491     678 
   Size:        140     351     187

  Fragments arranged by size: 

                 351     187     140
 
SapI GCTCTTCn'nnn_

Cuts at:      0      82     678 
   Size:         82     596
 
SexAI A'CCwGG_T

Cuts at:      0     434     678 
   Size:        434     244
 
SfcI C'TryA_G

Cuts at:      0     283     645     678 
   Size:        283     362      33

  Fragments arranged by size: 

                 362     283      33
 
StyI C'CwwG_G

Cuts at:      0     516     678 
   Size:        516     162
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     235     678 
   Size:        235     443
 

 Enzymes that do cut:

     AfeI     AloI     AvaI     BanI    BanII     BbsI     BcgI     BlpI
     BmrI     BpmI   Bpu10I    BseYI     BsgI  BsiHKAI Bsp1286I    BspMI
   BssHII   BstAPI    BstXI    BstYI     BtgI     DrdI     EarI     EciI
  Eco57MI EcoO109I    EcoRV    HaeII    Hin4I   HincII     MmeI   MspA1I
     NcoI     NspI    PpuMI     PstI    PvuII    SacII    SanDI     SapI
    SexAI     SfcI     StyI    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI    BbvCI
    BciVI     BclI    BfrBI     BglI    BglII Bme1580I    BmgBI     BmtI
     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI
    BsrDI    BsrFI    BsrGI    BssSI    BstBI   BstEII  BstZ17I   Bsu36I
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     EaeI     EagI
  EcoICRI    EcoNI    EcoRI     FalI     FseI     FspI    FspAI  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MscI     MslI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI     SalI
     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
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