Restriction map for pF1KE3935
##################################
# NO CUT ENZYMES (  148 enzymes) #
##################################

AatII       AccII       AccIII      AcyI        AflII       
AflIII      AgeI        AhdI        Alw44I      Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     AspEI       AspHI       AspI        AvrII       
BalI        BamHI       BanII       BbeI        BbrPI       
BcgI        BcnI        BfrI        BglI        BglII       
BlnI        BlpI        BmyI        Bpu1102I    BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BsiHKAI     BsiWI       BsmBI       BsmI        Bsp1286I    
Bsp1407I    BspDI       BspEI       BspLU11I    BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstXI       BstYI       BstZ17I     CelII       
Cfr10I      ClaI        CpoI        DraI        DrdI        
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoNI       EcoO65I     EcoRI       EcoT22I     
FseI        HaeII       HapII       HgaI        Hin1I       
HincII      HindII      HindIII     HpaI        HpaII       
KasI        KspI        MamI        MfeI        MflI        
MluI        MluNI       MroI        MscI        MspI        
MunI        MvnI        NaeI        NarI        NciI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PflFI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
PvuI        PvuII       RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
StuI        SwaI        Tth111I     XbaI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

Acc65I        495  GGTACC               G^GTACC
AccI          163  GTCTAC               GT^[AC][GT]AC
AclI          330  AACGTT               AA^CGTT
Asp718I       495  GGTACC               G^GTACC
AviII        1106  TGCGCA               TGC^GCA
BanI          495  GGTACC               G^G[CT][GA]CC
BbvCI         889  CCTCAGC              CCTCAGC(-5/-2)
BciVI         806  GGATAC               GTATCC(6/5)
BclI          522  TGATCA               T^GATCA
BfaI           17  CTAG                 C^TAG
BsgI         1351  GTGCAG               GTGCAG(16/14)
BsiEI        1263  CGACCG               CG[GA][CT]^CG
BspHI        1044  TCATGA               T^CATGA
BstF5I        675  GGATG                GGATG(2/0)
DraIII        779  CACCCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoRV         385  GATATC               GAT^ATC
EcoT14I       123  CCATGG               C^C[AT][AT]GG
FbaI          522  TGATCA               T^GATCA
FokI          675  GGATG                GGATG(9/13)
FspI         1106  TGCGCA               TGC^GCA
KpnI          495  GGTACC               GGTAC^C
MaeI           17  CTAG                 C^TAG
MslI          235  CATGTTGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        892  CAGCGG               C[AC]G^C[GT]G
NcoI          123  CCATGG               C^CATGG
NspI           83  ACATGC               [GA]CATG^[CT]
Psp1406I      330  AACGTT               AA^CGTT
PstI          704  CTGCAG               CTGCA^G
RcaI         1044  TCATGA               T^CATGA
SfcI          704  CTGCAG               C^T[GA][CT]AG
SspI          925  AATATT               AAT^ATT
StyI          123  CCATGG               C^C[AT][AT]GG
XcmI         1020  CCAACTCTTCATTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AciI           36  GCGG                 CCGC(-3/-1)
              894  GCGG                
AcsI           40  GAATTT               [GA]^AATT[CT]
              367  AAATTC              
AlwI          589  GATCC                GGATC(4/5)
              897  GGATC               
ApoI           40  GAATTT               [GA]^AATT[CT]
              367  AAATTC              
AvaI         1244  CTCGAG               C^[CT]CG[GA]G
             1301  CTCGAG              
BbsI          730  GAAGAC               GAAGAC(2/6)
             1239  GAAGAC              
BbvI           87  GCTGC                GCAGC(8/12)
             1249  GCTGC               
BpmI          695  CTCCAG               CTGGAG(16/14)
             1292  CTGGAG              
BpuAI         730  GAAGAC               GAAGAC(2/6)
             1239  GAAGAC              
BsmAI          48  GAGAC                GTCTC(1/5)
             1002  GAGAC               
BsoBI        1244  CTCGAG               C^[CT]CG[GA]G
             1301  CTCGAG              
BspMI         702  ACCTGC               ACCTGC(4/8)
             1037  ACCTGC              
Cac8I         150  GCCAGC               GC[ACGT]^[ACGT]GC
             1383  GCTTGC              
CfoI         1107  GCGC                 GCG^C
             1387  GCGC                
FauI           36  GCGGG                CCCGC(4/6)
              894  GCGGG               
Fnu4HI         87  GCTGC                GC^[ACGT]GC
             1249  GCTGC               
HhaI         1107  GCGC                 GCG^C
             1387  GCGC                
HinP1I       1107  GCGC                 G^CGC
             1387  GCGC                
ItaI           87  GCTGC                GC^[ACGT]GC
             1249  GCTGC               
MaeII         331  ACGT                 A^CGT
             1094  ACGT                
MwoI           27  GCTGGTCGGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              706  GCAGACATAGC         
PaeR7I       1244  CTCGAG               C^TCGAG
             1301  CTCGAG              
PpuMI         942  GGGACCC              [GA]G^G[AT]CC[CT]
             1287  GGGACCT             
TseI           87  GCTGC                G^C[AT]GC
             1249  GCTGC               
XhoI         1244  CTCGAG               C^TCGAG
             1301  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (   52 enzymes) #
#########################################

AfaI        AluI        AlwNI       AocI        AvaII       
BsaJI       BseRI       BsiYI       BslI        BsmFI       
BsrI        BssKI       BstNI       Bsu36I      Cfr13I      
DdeI        DpnII       DraII       DsaI        EarI        
Eco81I      EcoO109I    EcoRII      HaeIII      HinfI       
HphI        Ksp632I     MaeIII      MboI        MboII       
MnlI        MseI        MvaI        NdeII       NlaIII      
NlaIV       PflMI       PleI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SmlI        TaqI        
TfiI        Tru9I       Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      
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