Restriction map for pF1KE0425
##################################
# NO CUT ENZYMES (  131 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        AspEI       AviII       AvrII       
BalI        BamHI       BbrPI       BbvCI       BciVI       
BcnI        BfrI        BlnI        BlpI        BpmI        
Bpu1102I    BsaAI       BsaWI       BseAI       BseRI       
BsiEI       BsiWI       BsmBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstUI       BstXI       BstZ17I     
Bsu36I      CelII       Cfr10I      ClaI        CpoI        
DraI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoRI       
EcoT22I     FseI        FspI        HapII       HindIII     
HpaII       Ksp632I     KspI        MfeI        MluI        
MluNI       MroI        MscI        MspI        MunI        
MvnI        NaeI        NciI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflMI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SphI        Sse8387I    SspBI       SspI        
SwaI        Van91I      XbaI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

Acc65I        633  GGTACC               G^GTACC
AcsI          573  GAATTT               [GA]^AATT[CT]
ApoI          573  GAATTT               [GA]^AATT[CT]
Asp718I       633  GGTACC               G^GTACC
AspHI         343  GAGCTC               G[AT]GC[AT]^C
AspI           31  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          764  CCCGAG               C^[CT]CG[GA]G
BbeI          553  GGCGCC               GGCGC^C
BbsI          478  GAAGAC               GAAGAC(2/6)
BclI          938  TGATCA               T^GATCA
BfaI         1054  CTAG                 C^TAG
BglII         527  AGATCT               A^GATCT
BpuAI         478  GAAGAC               GAAGAC(2/6)
BsaBI         933  GATGGTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI           29  GAGACC               GGTCTC(1/5)
BsgI         1087  GTGCAG               GTGCAG(16/14)
BsiHKAI       343  GAGCTC               G[AT]GC[AT]^C
BsmFI         620  GGGAC                GGGAC(10/14)
BsmI           75  GAATGC               GAATGC(1/-1)
BsoBI         764  CCCGAG               C^[CT]CG[GA]G
BspMI        1103  ACCTGC               ACCTGC(4/8)
BstEII        247  GGTGACC              G^GT[ACGT]ACC
BstPI         247  GGTGACC              G^GT[ACGT]ACC
CfoI          554  GCGC                 GCG^C
DrdI          828  GACATTCCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI         642  CCTCATCGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       247  GGTGACC              G^GT[ACGT]ACC
FbaI          938  TGATCA               T^GATCA
HaeII         553  GGCGCC               [GA]GCGC^[CT]
HhaI          554  GCGC                 GCG^C
HinP1I        554  GCGC                 G^CGC
HincII         94  GTTAAC               GT[CT]^[GA]AC
HindII         94  GTTAAC               GT[CT]^[GA]AC
HpaI           94  GTTAAC               GTT^AAC
KasI          553  GGCGCC               G^GCGCC
KpnI          633  GGTACC               GGTAC^C
MaeI         1054  CTAG                 C^TAG
MaeII         725  ACGT                 A^CGT
MamI          933  GATGGTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI          553  GGCGCC               GG^CGCC
PflFI          31  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI         620  GGGACCC              [GA]G^G[AT]CC[CT]
SacI          343  GAGCTC               GAGCT^C
SfoI          553  GGCGCC               GGC^GCC
SpeI         1053  ACTAGT               A^CTAGT
StuI          852  AGGCCT               AGG^CCT
Tth111I        31  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI         1028  CCAAAAAGGCCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AcyI          454  GGCGTC               G[GA]^CG[CT]C
              553  GGCGCC              
AlwI          562  GATCC                GGATC(4/5)
              267  GGATC               
AlwNI         166  CAGTTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              265  CAGGATCTG           
Asp700I       478  GAAGACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              570  GAAGAATTTC          
BcgI          119  GCACAGTGATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1089  GCAGAGCTTTCG        
BglI          596  GCCCTCATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1027  GCCAAAAAGGC         
BsaHI         454  GGCGTC               G[GA]^CG[CT]C
              553  GGCGCC              
BstYI         267  GGATCT               [GA]^GATC[CT]
              527  AGATCT              
DdeI          524  CTAAG                C^T[ACGT]AG
              566  CTGAG               
DraII         620  GGGACCC              [GA]G^G[ACGT]CC[CT]
              866  AGGCCCC             
DraIII        154  CACATGGTG            CAC[ACGT][ACGT][ACGT]^GTG
              776  CACTGGGTG           
EcoO109I      620  GGGACCC              [GA]G^G[ACGT]CC[CT]
              866  AGGCCCC             
EcoRV         202  GATATC               GAT^ATC
              460  GATATC              
FauI          624  CCCGC                CCCGC(4/6)
              981  GCGGG               
HgaI          313  GCGTC                GACGC(5/10)
              455  GCGTC               
Hin1I         454  GGCGTC               G[GA]^CG[CT]C
              553  GGCGCC              
MflI          267  GGATCT               [GA]^GATC[CT]
              527  AGATCT              
MslI          711  CACCTTTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              972  CACACCCATG          
MspA1I        955  CAGCTG               C[AC]G^C[GT]G
             1014  CAGCTG              
PleI           45  GACTC                GAGTC(4/5)
              769  GACTC               
PstI          255  CTGCAG               CTGCA^G
              958  CTGCAG              
PvuII         955  CAGCTG               CAG^CTG
             1014  CAGCTG              
TfiI          102  GAATC                G^A[AT]TC
              140  GATTC               
XhoII         267  GGATCT               [GA]^GATC[CT]
              527  AGATCT              
XmnI          478  GAAGACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              570  GAAGAATTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   54 enzymes) #
#########################################

AciI        AfaI        AluI        AvaII       BanI        
BanII       BbvI        BmyI        BsaJI       BsiYI       
BslI        BsmAI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       Cac8I       Cfr13I      DpnII       
DsaI        EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HinfI       HphI        ItaI        MaeIII      
MboI        MboII       MnlI        MseI        MvaI        
MwoI        NcoI        NdeII       NlaIII      NlaIV       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        StyI        TaqI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     
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