Restriction map for pF1KB0452
 (Linear) (Six-base) MAPSORT of: KIBB0452.seq  Check: 9693  from: 1  to: 2757



KIBB0452.orf

 With 182 enzymes: SgfI * 

                        November 15, 2011 13:19  ..

AccI GT'mk_AC

Cuts at:      0      29    1021    2757 
   Size:         29     992    1736

  Fragments arranged by size: 

                1736     992      29
 
Acc65I G'GTAC_C

Cuts at:      0    1505    2757 
   Size:       1505    1252
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     559     923    1781    1912    2421    2757 
   Size:        559     364     858     131     509     336

  Fragments arranged by size: 

                 858     559     509     364     336     131
 
AfeI AGC'GCT

Cuts at:      0     984    2757 
   Size:        984    1773
 
AflII C'TTAA_G

Cuts at:      0     513    2757 
   Size:        513    2244
 
AflIII A'CryG_T

Cuts at:      0    1723    2139    2184    2757 
   Size:       1723     416      45     573

  Fragments arranged by size: 

                1723     573     416      45
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     442    2621    2757 
   Size:        442    2179     136

  Fragments arranged by size: 

                2179     442     136
 
AlwNI CAG_nnn'CTG

Cuts at:      0      75     397     438    1187    1464    1974    2757 
   Size:         75     322      41     749     277     510     783

  Fragments arranged by size: 

                 783     749     510     322     277      75      41
 
ApaI G_GGCC'C

Cuts at:      0     115     499    1573    2757 
   Size:        115     384    1074    1184

  Fragments arranged by size: 

                1184    1074     384     115
 
ApoI r'AATT_y

Cuts at:      0    1813    2407    2473    2757 
   Size:       1813     594      66     284

  Fragments arranged by size: 

                1813     594     284      66
 
AvaI C'yCGr_G

Cuts at:      0     108     373     636    2757 
   Size:        108     265     263    2121

  Fragments arranged by size: 

                2121     265     263     108
 
AvrII C'CTAG_G

Cuts at:      0     966    2757 
   Size:        966    1791
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1759    1792    2757 
   Size:       1759      33     965

  Fragments arranged by size: 

                1759     965      33
 
BanI G'GyrC_C

Cuts at:      0     119     151    1505    2363    2757 
   Size:        119      32    1354     858     394

  Fragments arranged by size: 

                1354     858     394     119      32
 
BanII G_rGCy'C

Cuts at:      0     115     499     645    1000    1573    1719    1884    2231 
   Size:        115     384     146     355     573     146     165     347

Cuts at:   2231    2757 
   Size:        526

  Fragments arranged by size: 

                 573     526     384     355     347     165     146     146
                 115
 
BbeI G_GCGC'C

Cuts at:      0     155    2757 
   Size:        155    2602
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1675    1734    1837    2757 
   Size:       1675      59     103     920

  Fragments arranged by size: 

                1675     920     103      59
 
BbvCI CC'TCA_GC

Cuts at:      0    1275    2757 
   Size:       1275    1482
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1045    1079    2071    2105    2598    2632    2757 
   Size:       1045      34     992      34     493      34     125

  Fragments arranged by size: 

                1045     992     493     125      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     609    2757 
   Size:        609    2148
 
BglI GCCn_nnn'nGGC

Cuts at:      0     308     409     640     853    1192    1238    2757 
   Size:        308     101     231     213     339      46    1519

  Fragments arranged by size: 

                1519     339     308     231     213     101      46
 
Bme1580I G_kGCm'C

Cuts at:      0     115     499     679     849    1535    1573    2711    2757 
   Size:        115     384     180     170     686      38    1138      46

  Fragments arranged by size: 

                1138     686     384     180     170     115      46      38
            
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1535    2128    2757 
   Size:       1535     593     629

  Fragments arranged by size: 

                1535     629     593
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     480    2712    2757 
   Size:        480    2232      45

  Fragments arranged by size: 

                2232     480      45
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     382     419    1180    2259    2757 
   Size:        382      37     761    1079     498

  Fragments arranged by size: 

                1079     761     498     382      37
 
Bpu10I CC'TnA_GC

Cuts at:      0     547    1275    2757 
   Size:        547     728    1482

  Fragments arranged by size: 

                1482     728     547
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2357    2541    2757 
   Size:       2357     184     216

  Fragments arranged by size: 

                2357     216     184
 
BsaAI yAC'GTr

Cuts at:      0    2142    2187    2757 
   Size:       2142      45     570

  Fragments arranged by size: 

                2142     570      45
 
BsaHI Gr'CG_yC

Cuts at:      0     152    2757 
   Size:        152    2605
 
BsaWI w'CCGG_w

Cuts at:      0     450    1042    1591    2673    2757 
   Size:        450     592     549    1082      84

  Fragments arranged by size: 

                1082     592     549     450      84
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     392     422    2757 
   Size:        392      30    2335

  Fragments arranged by size: 

                2335     392      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      74    1972    2757 
   Size:         74    1898     785

  Fragments arranged by size: 

                1898     785      74
 
BseYI C'CCAG_C

Cuts at:      0      18     492    2757 
   Size:         18     474    2265

  Fragments arranged by size: 

                2265     474      18
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      28    1268    2617    2757 
   Size:         28    1240    1349     140

  Fragments arranged by size: 

                1349    1240     140      28
 
BsiEI CG_ry'CG

Cuts at:      0      40    2517    2757 
   Size:         40    2477     240

  Fragments arranged by size: 

                2477     240      40
 
BsiHKAI G_wGCw'C

Cuts at:      0     901    1000    1050    1719    2757 
   Size:        901      99      50     669    1038

  Fragments arranged by size: 

                1038     901     669      99      50
 
BsmI GAATG_Cn'

Cuts at:      0     865    2757 
   Size:        865    1892
 
Bsp1286I G_dGCh'C

Cuts at:      0     115     499     645     679     849     901    1000    1050 
   Size:        115     384     146      34     170      52      99      50

Cuts at:   1050    1535    1573    1719    1884    2231    2711    2757 
   Size:        485      38     146     165     347     480      46

  Fragments arranged by size: 

                 485     480     384     347     170     165     146     146
                 115      99      52      50      46      38      34
 
BspEI T'CCGG_A

Cuts at:      0     450    1042    1591    2673    2757 
   Size:        450     592     549    1082      84

  Fragments arranged by size: 

                1082     592     549     450      84
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1681    2645    2757 
   Size:       1681     964     112

  Fragments arranged by size: 

                1681     964     112
 
BsrDI GCAATG_nn'

Cuts at:      0    2522    2757 
   Size:       2522     235
 
BsrGI T'GTAC_A

Cuts at:      0    2136    2339    2757 
   Size:       2136     203     418

  Fragments arranged by size: 

                2136     418     203
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     438    2757 
   Size:        438    2319
 
BstBI TT'CG_AA

Cuts at:      0    2490    2757 
   Size:       2490     267
 
BstEII G'GTnAC_C

Cuts at:      0    1268    2757 
   Size:       1268    1489
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     738    1333    2586    2757 
   Size:        738     595    1253     171

  Fragments arranged by size: 

                1253     738     595     171
 
BstYI r'GATC_y

Cuts at:      0    2714    2757 
   Size:       2714      43
 
Bsu36I CC'TnA_GG

Cuts at:      0    1457    2757 
   Size:       1457    1300
 
BtgI C'CryG_G

Cuts at:      0     407     731    2243    2757 
   Size:        407     324    1512     514

  Fragments arranged by size: 

                1512     514     407     324
 
BtsI GCAGTG_nn'

Cuts at:      0     725    1213    2425    2757 
   Size:        725     488    1212     332

  Fragments arranged by size: 

                1212     725     488     332
 
DraIII CAC_nnn'GTG

Cuts at:      0    2658    2757 
   Size:       2658      99
 
EaeI y'GGCC_r

Cuts at:      0      37     410     854    1106    2757 
   Size:         37     373     444     252    1651

  Fragments arranged by size: 

                1651     444     373     252      37
 
EagI C'GGCC_G

Cuts at:      0      37    2757 
   Size:         37    2720
 
EarI CTCTTCn'nnn_

Cuts at:      0    1312    2440    2757 
   Size:       1312    1128     317

  Fragments arranged by size: 

                1312    1128     317
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1115    2757 
   Size:       1115    1642
 
EcoICRI GAG'CTC

Cuts at:      0     998    1717    2757 
   Size:        998     719    1040

  Fragments arranged by size: 

                1040     998     719
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     382     419     559     923    1180    1781    1912    2259 
   Size:        382      37     140     364     257     601     131     347

Cuts at:   2259    2421    2757 
   Size:        162     336

  Fragments arranged by size: 

                 601     382     364     347     336     257     162     140
                 131      37
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     325     550    2757 
   Size:        325     225    2207

  Fragments arranged by size: 

                2207     325     225
 
EcoO109I rG'GnC_Cy

Cuts at:      0     112     301     496    1221    1255    1570    2757 
   Size:        112     189     195     725      34     315    1187

  Fragments arranged by size: 

                1187     725     315     195     189     112      34
 
EcoRI G'AATT_C

Cuts at:      0    2407    2757 
   Size:       2407     350
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1761    2757 
   Size:       1761     996
 
HaeII r_GCGC'y

Cuts at:      0     155     986    1187    2757 
   Size:        155     831     201    1570

  Fragments arranged by size: 

                1570     831     201     155
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     371     403    1573    1605    2757 
   Size:        371      32    1170      32    1152

  Fragments arranged by size: 

                1170    1152     371      32      32
 
HincII GTy'rAC

Cuts at:      0    2271    2757 
   Size:       2271     486
 
HindIII A'AGCT_T

Cuts at:      0    1694    2757 
   Size:       1694    1063
 
KasI G'GCGC_C

Cuts at:      0     151    2757 
   Size:        151    2606
 
KpnI G_GTAC'C

Cuts at:      0    1509    2757 
   Size:       1509    1248
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     734    2757 
   Size:        734    2023
 
MslI CAynn'nnrTG

Cuts at:      0    2698    2757 
   Size:       2698      59
 
MspA1I CmG'CkG

Cuts at:      0     438     469     521     529     617     826    1063    1170 
   Size:        438      31      52       8      88     209     237     107

Cuts at:   1170    1252    1759    2757 
   Size:         82     507     998

  Fragments arranged by size: 

                 998     507     438     237     209     107      88      82
                  52      31       8
 
NarI GG'CG_CC

Cuts at:      0     152    2757 
   Size:        152    2605
 
NcoI C'CATG_G

Cuts at:      0     731    2243    2757 
   Size:        731    1512     514

  Fragments arranged by size: 

                1512     731     514
 
NruI TCG'CGA

Cuts at:      0    1079    2757 
   Size:       1079    1678
 
NspI r_CATG'y

Cuts at:      0     798    1611    1727    2325    2531    2757 
   Size:        798     813     116     598     206     226

  Fragments arranged by size: 

                 813     798     598     226     206     116
 
PciI A'CATG_T

Cuts at:      0    1723    2757 
   Size:       1723    1034
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     397    2249    2455    2757 
   Size:        397    1852     206     302

  Fragments arranged by size: 

                1852     397     302     206
 
PfoI T'CCnGG_A

Cuts at:      0     320    1000    2027    2218    2275    2332    2585    2757 
   Size:        320     680    1027     191      57      57     253     172

  Fragments arranged by size: 

                1027     680     320     253     191     172      57      57
            
 
PmlI CAC'GTG

Cuts at:      0    2142    2187    2757 
   Size:       2142      45     570

  Fragments arranged by size: 

                2142     570      45
 
PpuMI rG'GwC_Cy

Cuts at:      0    1221    1255    2757 
   Size:       1221      34    1502

  Fragments arranged by size: 

                1502    1221      34
 
PspOMI G'GGCC_C

Cuts at:      0     111     495    1569    2757 
   Size:        111     384    1074    1188

  Fragments arranged by size: 

                1188    1074     384     111
 
PstI C_TGCA'G

Cuts at:      0     173     990    2757 
   Size:        173     817    1767

  Fragments arranged by size: 

                1767     817     173
 
PvuI CG_AT'CG

Cuts at:      0    2517    2757 
   Size:       2517     240
 
PvuII CAG'CTG

Cuts at:      0     438     469     521    1063    2757 
   Size:        438      31      52     542    1694

  Fragments arranged by size: 

                1694     542     438      52      31
 
RsrII CG'GwC_CG

Cuts at:      0    1111    2757 
   Size:       1111    1646
 
SacI G_AGCT'C

Cuts at:      0    1000    1719    2757 
   Size:       1000     719    1038

  Fragments arranged by size: 

                1038    1000     719
 
SanDI GG'GwC_CC

Cuts at:      0    1255    2757 
   Size:       1255    1502
 
ScaI AGT'ACT

Cuts at:      0    2215    2757 
   Size:       2215     542
 
SexAI A'CCwGG_T

Cuts at:      0     314    2757 
   Size:        314    2443
 
SfcI C'TryA_G

Cuts at:      0     169     986    1427    1915    2757 
   Size:        169     817     441     488     842

  Fragments arranged by size: 

                 842     817     488     441     169
 
SfoI GGC'GCC

Cuts at:      0     153    2757 
   Size:        153    2604
 
SmaI CCC'GGG

Cuts at:      0     110    2757 
   Size:        110    2647
 
SmlI C'TyrA_G

Cuts at:      0     513    2372    2556    2757 
   Size:        513    1859     184     201

  Fragments arranged by size: 

                1859     513     201     184
 
SphI G_CATG'C

Cuts at:      0    2325    2531    2757 
   Size:       2325     206     226

  Fragments arranged by size: 

                2325     226     206
 
StuI AGG'CCT

Cuts at:      0    2161    2757 
   Size:       2161     596
 
StyI C'CwwG_G

Cuts at:      0     656     701     731     966    2156    2243    2757 
   Size:        656      45      30     235    1190      87     514

  Fragments arranged by size: 

                1190     656     514     235      87      45      30
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     137     730    1691    2757 
   Size:        137     593     961    1066

  Fragments arranged by size: 

                1066     961     593     137
 
TatI w'GTAC_w

Cuts at:      0    2136    2213    2339    2757 
   Size:       2136      77     126     418

  Fragments arranged by size: 

                2136     418     126      77
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     392     424    2398    2430    2757 
   Size:        392      32    1974      32     327

  Fragments arranged by size: 

                1974     392     327      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1980    2757 
   Size:       1980     777
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     312    2757 
   Size:        312    2445
 
XmaI C'CCGG_G

Cuts at:      0     108    2757 
   Size:        108    2649
 
XmnI GAAnn'nnTTC

Cuts at:      0      76     779    2757 
   Size:         76     703    1978

  Fragments arranged by size: 

                1978     703      76
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI     AfeI    AflII   AflIII     AlfI    AlwNI
     ApaI     ApoI     AvaI    AvrII     BaeI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BglI Bme1580I     BmrI     BplI
     BpmI   Bpu10I    BpuEI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspMI
    BsrDI    BsrGI   BstAPI    BstBI   BstEII    BstXI    BstYI   Bsu36I
     BtgI     BtsI   DraIII     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI     FalI    HaeII    Hin4I   HincII
  HindIII     KasI     KpnI     MmeI     MslI   MspA1I     NarI     NcoI
     NruI     NspI     PciI    PflMI     PfoI     PmlI    PpuMI   PspOMI
     PstI     PvuI    PvuII    RsrII     SacI    SanDI     ScaI    SexAI
     SfcI     SfoI     SmaI     SmlI     SphI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AgeI     AhdI     AleI     AloI    ApaLI
     AscI     AseI    AsiSI    BamHI     BclI    BfrBI    BglII     BlpI
    BmgBI     BmtI     BsaI    BsaBI    BsiWI    BsmBI    BspHI    BsrBI
    BsrFI   BssHII    BssSI  BstZ17I    BtgZI     ClaI    CspCI     DraI
     DrdI    EcoRV     FseI     FspI    FspAI     HpaI     MfeI     MluI
     MscI     NaeI     NdeI   NgoMIV     NheI     NotI     NsiI     PacI
     PasI     PmeI     PpiI    PshAI     PsiI    PspXI     PsrI    SacII
     SalI     SapI     SbfI     SfiI     SgfI    SgrAI    SnaBI     SpeI
     SrfI     SspI     SwaI     XbaI     XhoI     ZraI
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