Restriction map for
pF1KE0387
(Circular) (Six-base) MAPSORT of: pF1KE0387.seq Check: 3639 from: 1 to: 4922
pF1KE0387 4922 bp
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1846 1846
Size: 4922
AccI GT'mk_AC
Cuts at: 279 656 1960 279
Size: 377 1304 3241
Fragments arranged by size:
3241 1304 377
Acc65I G'GTAC_C
Cuts at: 1938 1938
Size: 4922
AclI AA'CG_TT
Cuts at: 292 1916 4762 292
Size: 1624 2846 452
Fragments arranged by size:
2846 1624 452
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 340 648 703 2747 3179 3926 4194 340
Size: 308 55 2044 432 747 268 1068
Fragments arranged by size:
2044 1068 747 432 308 268 55
AfeI AGC'GCT
Cuts at: 523 523
Size: 4922
AflII C'TTAA_G
Cuts at: 1276 1276
Size: 4922
AflIII A'CryG_T
Cuts at: 2274 3378 4355 2274
Size: 1104 977 2841
Fragments arranged by size:
2841 1104 977
AgeI A'CCGG_T
Cuts at: 3310 3310
Size: 4922
AlwNI CAG_nnn'CTG
Cuts at: 318 1869 2227 3794 318
Size: 1551 358 1567 1446
Fragments arranged by size:
1567 1551 1446 358
ApaLI G'TGCA_C
Cuts at: 3692 3692
Size: 4922
ApoI r'AATT_y
Cuts at: 1450 1903 1926 4275 4531 1450
Size: 453 23 2349 256 1841
Fragments arranged by size:
2349 1841 453 256 23
AseI AT'TA_AT
Cuts at: 20 20
Size: 4922
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4922
AvaI C'yCGr_G
Cuts at: 1942 1942
Size: 4922
BamHI G'GATC_C
Cuts at: 995 1947 995
Size: 952 3970
BanI G'GyrC_C
Cuts at: 220 249 500 1938 2352 2600 2635 220
Size: 29 251 1438 414 248 35 2507
Fragments arranged by size:
2507 1438 414 251 248 35 29
BanII G_rGCy'C
Cuts at: 271 1472 1549 1936 2966 271
Size: 1201 77 387 1030 2227
Fragments arranged by size:
2227 1201 1030 387 77
BbeI G_GCGC'C
Cuts at: 253 2356 2604 253
Size: 2103 248 2571
Fragments arranged by size:
2571 2103 248
BbsI GAAGACnn'nnnn_
Cuts at: 393 704 393
Size: 311 4611
BciVI GTATCCnnnnn_n'
Cuts at: 2820 3581 4922 2820
Size: 761 1341 2820
Fragments arranged by size:
2820 1341 761
BclI T'GATC_A
Cuts at: 2442 2442
Size: 4922
BglI GCCn_nnn'nGGC
Cuts at: 787 787
Size: 4922
BglII A'GATC_T
Cuts at: 1127 2437 4474 1127
Size: 1310 2037 1575
Fragments arranged by size:
2037 1575 1310
BlpI GC'TnA_GC
Cuts at: 359 2034 359
Size: 1675 3247
Bme1580I G_kGCm'C
Cuts at: 898 1238 1510 2547 2640 3696 898
Size: 340 272 1037 93 1056 2124
Fragments arranged by size:
2124 1056 1037 340 272 93
BmrI ACTGGGnnnn_n'
Cuts at: 713 803 984 2134 2215 2550 4694 713
Size: 90 181 1150 81 335 2144 941
Fragments arranged by size:
2144 1150 941 335 181 90 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 374 3289 374
Size: 2915 2007
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 550 611 992 1188 1647 1667 550
Size: 61 381 196 459 20 3805
Fragments arranged by size:
3805 459 381 196 61 20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2098 2977 3469 3767 2098
Size: 879 492 298 3253
Fragments arranged by size:
3253 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1643 4626 1643
Size: 2983 1939
BsaAI yAC'GTr
Cuts at: 2275 2275
Size: 4922
BsaBI GATnn'nnATC
Cuts at: 6 1000 2436 4473 6
Size: 994 1436 2037 455
Fragments arranged by size:
2037 1436 994 455
BsaHI Gr'CG_yC
Cuts at: 250 2353 2601 3303 4799 250
Size: 2103 248 702 1496 373
Fragments arranged by size:
2103 1496 702 373 248
BsaWI w'CCGG_w
Cuts at: 347 2111 2632 3310 3584 3731 4142 347
Size: 1764 521 678 274 147 411 1127
Fragments arranged by size:
1764 1127 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 580 580
Size: 4922
BseYI C'CCAG_C
Cuts at: 774 1030 1357 1655 1859 2347 3105 3682 4178
Size: 256 327 298 204 488 758 577 496
Cuts at: 4178 774
Size: 1518
Fragments arranged by size:
1518 758 577 496 488 327 298 256
204
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1541 1541
Size: 4922
BsiEI CG_ry'CG
Cuts at: 104 1253 2464 2510 3718 104
Size: 1149 1211 46 1208 1308
Fragments arranged by size:
1308 1211 1208 1149 46
BsiHKAI G_wGCw'C
Cuts at: 614 680 1936 2714 2904 3696 614
Size: 66 1256 778 190 792 1840
Fragments arranged by size:
1840 1256 792 778 190 66
BsmI GAATG_Cn'
Cuts at: 776 776
Size: 4922
Bsp1286I G_dGCh'C
Cuts at: 271 614 680 898 1238 1472 1510 1549 1936
Size: 343 66 218 340 234 38 39 387
Cuts at: 1936 2547 2640 2714 2904 2966 3696 271
Size: 611 93 74 190 62 730 1497
Fragments arranged by size:
1497 730 611 387 343 340 234 218
190 93 74 66 62 39 38
BspEI T'CCGG_A
Cuts at: 347 4142 347
Size: 3795 1127
BspHI T'CATG_A
Cuts at: 1829 4098 1829
Size: 2269 2653
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1451 1599 1846 1972 2186 2488 2869 1451
Size: 148 247 126 214 302 381 3504
Fragments arranged by size:
3504 381 302 247 214 148 126
BsrBI CCG'CTC
Cuts at: 50 207 3214 3268 4752 4917 50
Size: 157 3007 54 1484 165 55
Fragments arranged by size:
3007 1484 165 157 55 54
BsrDI GCAATG_nn'
Cuts at: 866 2834 866
Size: 1968 2954
BsrFI r'CCGG_y
Cuts at: 252 1060 1331 2920 3101 3310 252
Size: 808 271 1589 181 209 1864
Fragments arranged by size:
1864 1589 808 271 209 181
BssSI C'ACGA_G
Cuts at: 3193 3551 3193
Size: 358 4564
BstAPI GCAn_nnn'nTGC
Cuts at: 952 1802 2299 952
Size: 850 497 3575
Fragments arranged by size:
3575 850 497
BstBI TT'CG_AA
Cuts at: 735 3283 735
Size: 2548 2374
BstYI r'GATC_y
Cuts at: 1 995 1127 1493 1823 1947 2414 2437 2772
Size: 994 132 366 330 124 467 23 335
Cuts at: 2772 3018 4030 4116 4128 4474 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 994 467 449 366 346 335 330
246 132 124 86 23 12
BtgI C'CryG_G
Cuts at: 1076 1463 1581 1076
Size: 387 118 4417
Fragments arranged by size:
4417 387 118
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2872 3053 3216 2872
Size: 181 163 4578
Fragments arranged by size:
4578 181 163
BtsI GCAGTG_nn'
Cuts at: 1812 1870 2165 1812
Size: 58 295 4569
Fragments arranged by size:
4569 295 58
DraI TTT'AAA
Cuts at: 1921 1921
Size: 4922
DraIII CAC_nnn'GTG
Cuts at: 609 826 609
Size: 217 4705
DrdI GACnn_nn'nnGTC
Cuts at: 2628 3486 2628
Size: 858 4064
EaeI y'GGCC_r
Cuts at: 1058 1250 1286 2507 2681 3072 3099 4444 1058
Size: 192 36 1221 174 391 27 1345 1536
Fragments arranged by size:
1536 1345 1221 391 192 174 36 27
EagI C'GGCC_G
Cuts at: 1250 2507 1250
Size: 1257 3665
EarI CTCTTCn'nnn_
Cuts at: 2945 3155 2945
Size: 210 4712
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 129 3130 3440 3586 4732 129
Size: 3001 310 146 1146 319
Fragments arranged by size:
3001 1146 319 310 146
EcoICRI GAG'CTC
Cuts at: 1934 1934
Size: 4922
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 340 550 611 648 703 992 1188 1647 1667
Size: 210 61 37 55 289 196 459 20
Cuts at: 1667 2747 3179 3926 4194 340
Size: 1080 432 747 268 1068
Fragments arranged by size:
1080 1068 747 459 432 289 268 210
196 61 55 37 20
EcoNI CCTnn'n_nnAGG
Cuts at: 1203 1203
Size: 4922
EcoO109I rG'GnC_Cy
Cuts at: 764 779 1245 1440 1538 2061 764
Size: 15 466 195 98 523 3625
Fragments arranged by size:
3625 523 466 195 98 15
EcoRI G'AATT_C
Cuts at: 1926 1926
Size: 4922
EcoRV GAT'ATC
Cuts at: 495 495
Size: 4922
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1692 2941 1692
Size: 1249 3673
FspI TGC'GCA
Cuts at: 2703 2703
Size: 4922
HaeII r_GCGC'y
Cuts at: 148 196 253 525 1332 2356 2604 3626 4605
Size: 48 57 272 807 1024 248 1022 979
Cuts at: 4605 148
Size: 465
Fragments arranged by size:
1024 1022 979 807 465 272 248 57
48
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 405 434 437 454 466 486 565 597 1622
Size: 29 3 17 12 20 79 32 1025
Cuts at: 1622 1654 2764 2794 2796 2826 405
Size: 32 1110 30 2 30 2501
Fragments arranged by size:
2501 1110 1025 79 32 32 30 30
29 20 17 12 3 2
HincII GTy'rAC
Cuts at: 280 657 840 1961 280
Size: 377 183 1121 3241
Fragments arranged by size:
3241 1121 377 183
KasI G'GCGC_C
Cuts at: 249 2352 2600 249
Size: 2103 248 2571
Fragments arranged by size:
2571 2103 248
KpnI G_GTAC'C
Cuts at: 1942 1942
Size: 4922
MluI A'CGCG_T
Cuts at: 4355 4355
Size: 4922
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3593 3777 3593
Size: 184 4738
MscI TGG'CCA
Cuts at: 2683 2683
Size: 4922
MslI CAynn'nnrTG
Cuts at: 2911 3038 2911
Size: 127 4795
MspA1I CmG'CkG
Cuts at: 1357 1743 1793 2033 2347 2707 3720 3965 1357
Size: 386 50 240 314 360 1013 245 2314
Fragments arranged by size:
2314 1013 386 360 314 245 240 50
NaeI GCC'GGC
Cuts at: 254 1333 3103 254
Size: 1079 1770 2073
Fragments arranged by size:
2073 1770 1079
NarI GG'CG_CC
Cuts at: 250 2353 2601 250
Size: 2103 248 2571
Fragments arranged by size:
2571 2103 248
NgoMIV G'CCGG_C
Cuts at: 252 1331 3101 252
Size: 1079 1770 2073
Fragments arranged by size:
2073 1770 1079
NspI r_CATG'y
Cuts at: 1608 1975 2475 3382 1608
Size: 367 500 907 3148
Fragments arranged by size:
3148 907 500 367
PasI CC'CwG_GG
Cuts at: 226 226
Size: 4922
PciI A'CATG_T
Cuts at: 3378 3378
Size: 4922
PmeI GTTT'AAAC
Cuts at: 1921 1921
Size: 4922
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4061 4093 4061
Size: 32 4890
PpuMI rG'GwC_Cy
Cuts at: 764 1245 1440 764
Size: 481 195 4246
Fragments arranged by size:
4246 481 195
PstI C_TGCA'G
Cuts at: 137 1019 1482 1807 1969 2654 137
Size: 882 463 325 162 685 2405
Fragments arranged by size:
2405 882 685 463 325 162
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4922
PvuII CAG'CTG
Cuts at: 1743 1793 2347 2707 1743
Size: 50 554 360 3958
Fragments arranged by size:
3958 554 360 50
RsrII CG'GwC_CG
Cuts at: 3117 3117
Size: 4922
SacI G_AGCT'C
Cuts at: 1936 1936
Size: 4922
SalI G'TCGA_C
Cuts at: 278 655 1959 278
Size: 377 1304 3241
Fragments arranged by size:
3241 1304 377
SapI GCTCTTCn'nnn_
Cuts at: 2945 3155 2945
Size: 210 4712
SbfI CC_TGCA'GG
Cuts at: 137 1969 137
Size: 1832 3090
ScaI AGT'ACT
Cuts at: 4439 4439
Size: 4922
SfcI C'TryA_G
Cuts at: 33 133 1015 1221 1478 1803 1965 2650 3643
Size: 100 882 206 257 325 162 685 993
Cuts at: 3643 3834 33
Size: 191 1121
Fragments arranged by size:
1121 993 882 685 325 257 206 191
162 100
SfoI GGC'GCC
Cuts at: 251 2354 2602 251
Size: 2103 248 2571
Fragments arranged by size:
2571 2103 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4922
SgrAI Cr'CCGG_yG
Cuts at: 252 252
Size: 4922
SmaI CCC'GGG
Cuts at: 1944 1944
Size: 4922
SmlI C'TyrA_G
Cuts at: 1276 2077 2992 3484 3746 1276
Size: 801 915 492 262 2452
Fragments arranged by size:
2452 915 801 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4922
SphI G_CATG'C
Cuts at: 1975 2475 1975
Size: 500 4422
StyI C'CwwG_G
Cuts at: 1541 2056 2410 1541
Size: 515 354 4053
Fragments arranged by size:
4053 515 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 418 518 1873 2505 2638 3097 3306 418
Size: 100 1355 632 133 459 209 2034
Fragments arranged by size:
2034 1355 632 459 209 133 100
TatI w'GTAC_w
Cuts at: 648 983 1316 4437 648
Size: 335 333 3121 1133
Fragments arranged by size:
3121 1133 335 333
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1072 1104 4439 4471 1072
Size: 32 3335 32 1523
Fragments arranged by size:
3335 1523 32 32
Tth111I GACn'n_nGTC
Cuts at: 2719 2719
Size: 4922
XbaI T'CTAG_A
Cuts at: 1953 1953
Size: 4922
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1354 1783 2417 1354
Size: 429 634 3859
Fragments arranged by size:
3859 634 429
XmaI C'CCGG_G
Cuts at: 1942 1942
Size: 4922
XmnI GAAnn'nnTTC
Cuts at: 1090 1090
Size: 4922
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PasI PciI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AhdI AleI AlfI AloI ApaI AscI AvrII
BaeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I BsaXI
BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PflMI PfoI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SnaBI SrfI SspI StuI SwaI XhoI
ZraI