Restriction map for pF1KE0386
 (Linear) (Six-base) MAPSORT of: KIEE0386.seq  Check: 9876  from: 1  to: 2082



KIEE0386.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:13  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1052    2082 
   Size:       1052    1030
 
AccI GT'mk_AC

Cuts at:      0     383     665    2082 
   Size:        383     282    1417

  Fragments arranged by size: 

                1417     383     282
 
Acc65I G'GTAC_C

Cuts at:      0    2076    2082 
   Size:       2076       6
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1684    1738    2082 
   Size:       1684      54     344

  Fragments arranged by size: 

                1684     344      54
 
AleI CACnn'nnGTG

Cuts at:      0     343     892    2082 
   Size:        343     549    1190

  Fragments arranged by size: 

                1190     549     343
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     361     982    2082 
   Size:        361     621    1100

  Fragments arranged by size: 

                1100     621     361
 
AlwNI CAG_nnn'CTG

Cuts at:      0     871    1526    1608    2082 
   Size:        871     655      82     474

  Fragments arranged by size: 

                 871     655     474      82
 
ApaI G_GGCC'C

Cuts at:      0     317     716    1904    2082 
   Size:        317     399    1188     178

  Fragments arranged by size: 

                1188     399     317     178
 
ApaLI G'TGCA_C

Cuts at:      0    1971    2082 
   Size:       1971     111
 
ApoI r'AATT_y

Cuts at:      0     802    2082 
   Size:        802    1280
 
AvaI C'yCGr_G

Cuts at:      0     745    2082 
   Size:        745    1337
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     675     708    2082 
   Size:        675      33    1374

  Fragments arranged by size: 

                1374     675      33
 
BamHI G'GATC_C

Cuts at:      0    1883    2082 
   Size:       1883     199
 
BanI G'GyrC_C

Cuts at:      0     466     936    2046    2076    2082 
   Size:        466     470    1110      30       6

  Fragments arranged by size: 

                1110     470     466      30       6
 
BanII G_rGCy'C

Cuts at:      0     317     716    1186    1412    1904    1958    2082 
   Size:        317     399     470     226     492      54     124

  Fragments arranged by size: 

                 492     470     399     317     226     124      54
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     909    1726    2082 
   Size:        909     817     356

  Fragments arranged by size: 

                 909     817     356
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1899    2082 
   Size:       1899     183
 
Bme1580I G_kGCm'C

Cuts at:      0      91     123     317     340     716    1904    1975    2051 
   Size:         91      32     194      23     376    1188      71      76

Cuts at:   2051    2082 
   Size:         31

  Fragments arranged by size: 

                1188     376     194      91      76      71      32      31
                  23
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1514    2082 
   Size:       1514     568
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1393    1431    2082 
   Size:       1393      38     651

  Fragments arranged by size: 

                1393     651      38
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     672     964    1054    1972    2082 
   Size:        672     292      90     918     110

  Fragments arranged by size: 

                 918     672     292     110      90
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     535     833    2082 
   Size:        535     298    1249

  Fragments arranged by size: 

                1249     535     298
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     300     375    2082 
   Size:        300      75    1707

  Fragments arranged by size: 

                1707     300      75
 
BsaAI yAC'GTr

Cuts at:      0     242    1440    1683    2082 
   Size:        242    1198     243     399

  Fragments arranged by size: 

                1198     399     243     242
 
BsaHI Gr'CG_yC

Cuts at:      0     209    2082 
   Size:        209    1873
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1086    1116    2082 
   Size:       1086      30     966

  Fragments arranged by size: 

                1086     966      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     733     856    2082 
   Size:        733     123    1226

  Fragments arranged by size: 

                1226     733     123
 
BseYI C'CCAG_C

Cuts at:      0     268     454     595     708    1299    1333    1385    1728 
   Size:        268     186     141     113     591      34      52     343

Cuts at:   1728    1763    2082 
   Size:         35     319

  Fragments arranged by size: 

                 591     343     319     268     186     141     113      52
                  35      34
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     978    2082 
   Size:        978    1104
 
BsiHKAI G_wGCw'C

Cuts at:      0    1412    1975    2082 
   Size:       1412     563     107

  Fragments arranged by size: 

                1412     563     107
 
BsiWI C'GTAC_G

Cuts at:      0     242    2082 
   Size:        242    1840
 
BsmI GAATG_Cn'

Cuts at:      0     787    1694    2082 
   Size:        787     907     388

  Fragments arranged by size: 

                 907     787     388
 
Bsp1286I G_dGCh'C

Cuts at:      0      91     123     317     340     716    1186    1412    1904 
   Size:         91      32     194      23     376     470     226     492

Cuts at:   1904    1958    1975    2051    2082 
   Size:         54      17      76      31

  Fragments arranged by size: 

                 492     470     376     226     194      91      76      54
                  32      31      23      17
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1052    1380    1927    2082 
   Size:       1052     328     547     155

  Fragments arranged by size: 

                1052     547     328     155
 
BsrDI GCAATG_nn'

Cuts at:      0     402    2082 
   Size:        402    1680
 
BsrFI r'CCGG_y

Cuts at:      0    1401    2082 
   Size:       1401     681
 
BsrGI T'GTAC_A

Cuts at:      0     878    2082 
   Size:        878    1204
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     157    1056    2082 
   Size:        157     899    1026

  Fragments arranged by size: 

                1026     899     157
 
BstYI r'GATC_y

Cuts at:      0     105     443    1883    2082 
   Size:        105     338    1440     199

  Fragments arranged by size: 

                1440     338     199     105
 
Bsu36I CC'TnA_GG

Cuts at:      0    1139    2082 
   Size:       1139     943
 
BtgI C'CryG_G

Cuts at:      0     150     278     332    2082 
   Size:        150     128      54    1750

  Fragments arranged by size: 

                1750     150     128      54
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     248    2082 
   Size:        248    1834
 
ClaI AT'CG_AT

Cuts at:      0     173    2082 
   Size:        173    1909
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      64    2082 
   Size:         64    2018
 
EaeI y'GGCC_r

Cuts at:      0     179    1229    1434    1511    2082 
   Size:        179    1050     205      77     571

  Fragments arranged by size: 

                1050     571     205     179      77
 
EarI CTCTTCn'nnn_

Cuts at:      0     638    1006    1580    2082 
   Size:        638     368     574     502

  Fragments arranged by size: 

                 638     574     502     368
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    2029    2082 
   Size:       2029      53
 
EcoICRI GAG'CTC

Cuts at:      0    1410    2082 
   Size:       1410     672
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     672     964    1054    1684    1738    1972    2082 
   Size:        672     292      90     630      54     234     110

  Fragments arranged by size: 

                 672     630     292     234     110      90      54
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     838    1827    2005    2082 
   Size:        838     989     178      77

  Fragments arranged by size: 

                 989     838     178      77
 
EcoO109I rG'GnC_Cy

Cuts at:      0     451     592     712    1044    1820    1901    1916    2082 
   Size:        451     141     120     332     776      81      15     166

  Fragments arranged by size: 

                 776     451     332     166     141     120      81      15
            
 
EcoRI G'AATT_C

Cuts at:      0     802    2082 
   Size:        802    1280
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1839    1992    2082 
   Size:       1839     153      90

  Fragments arranged by size: 

                1839     153      90
 
HaeII r_GCGC'y

Cuts at:      0    1402    2082 
   Size:       1402     680
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     280     312     435     467    2082 
   Size:        280      32     123      32    1615

  Fragments arranged by size: 

                1615     280     123      32      32
 
KpnI G_GTAC'C

Cuts at:      0    2080    2082 
   Size:       2080       2
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1992    2082 
   Size:       1992      90
 
MscI TGG'CCA

Cuts at:      0     181    1231    1436    1513    2082 
   Size:        181    1050     205      77     569

  Fragments arranged by size: 

                1050     569     205     181      77
 
MslI CAynn'nnrTG

Cuts at:      0     343     892    1442    2082 
   Size:        343     549     550     640

  Fragments arranged by size: 

                 640     550     549     343
 
MspA1I CmG'CkG

Cuts at:      0     113     343     458     599     972    1299    1767    2082 
   Size:        113     230     115     141     373     327     468     315

  Fragments arranged by size: 

                 468     373     327     315     230     141     115     113
            
 
NcoI C'CATG_G

Cuts at:      0     150    2082 
   Size:        150    1932
 
PasI CC'CwG_GG

Cuts at:      0    1959    2082 
   Size:       1959     123
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     218     221     461    1227    1647    2082 
   Size:        218       3     240     766     420     435

  Fragments arranged by size: 

                 766     435     420     240     218       3
 
PfoI T'CCnGG_A

Cuts at:      0    1070    1205    2082 
   Size:       1070     135     877

  Fragments arranged by size: 

                1070     877     135
 
PmlI CAC'GTG

Cuts at:      0    1440    2082 
   Size:       1440     642
 
PpuMI rG'GwC_Cy

Cuts at:      0     451    1044    1820    1916    2082 
   Size:        451     593     776      96     166

  Fragments arranged by size: 

                 776     593     451     166      96
 
PshAI GACnn'nnGTC

Cuts at:      0    2008    2082 
   Size:       2008      74
 
PspOMI G'GGCC_C

Cuts at:      0     313     712    1900    2082 
   Size:        313     399    1188     182

  Fragments arranged by size: 

                1188     399     313     182
 
PstI C_TGCA'G

Cuts at:      0     971     977    2082 
   Size:        971       6    1105

  Fragments arranged by size: 

                1105     971       6
 
PvuII CAG'CTG

Cuts at:      0     343     599     972    1767    2082 
   Size:        343     256     373     795     315

  Fragments arranged by size: 

                 795     373     343     315     256
 
SacI G_AGCT'C

Cuts at:      0    1412    2082 
   Size:       1412     670
 
SanDI GG'GwC_CC

Cuts at:      0     451    2082 
   Size:        451    1631
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1006    1580    2082 
   Size:       1006     574     502

  Fragments arranged by size: 

                1006     574     502
 
ScaI AGT'ACT

Cuts at:      0    1174    2082 
   Size:       1174     908
 
SfcI C'TryA_G

Cuts at:      0     782     967     973    2082 
   Size:        782     185       6    1109

  Fragments arranged by size: 

                1109     782     185       6
 
SgrAI Cr'CCGG_yG

Cuts at:      0    1401    2082 
   Size:       1401     681
 
SmlI C'TyrA_G

Cuts at:      0     514     848    2082 
   Size:        514     334    1234

  Fragments arranged by size: 

                1234     514     334
 
StuI AGG'CCT

Cuts at:      0     812    2082 
   Size:        812    1270
 
StyI C'CwwG_G

Cuts at:      0     150     588     638     734    1281    2082 
   Size:        150     438      50      96     547     801

  Fragments arranged by size: 

                 801     547     438     150      96      50
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     288    1111    2082 
   Size:        288     823     971

  Fragments arranged by size: 

                 971     823     288
 
TatI w'GTAC_w

Cuts at:      0     878    1172    2082 
   Size:        878     294     910

  Fragments arranged by size: 

                 910     878     294
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1086    1118    2082 
   Size:       1086      32     964

  Fragments arranged by size: 

                1086     964      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1116    2082 
   Size:       1116     966
 
XbaI T'CTAG_A

Cuts at:      0     375    2082 
   Size:        375    1707
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     276    2082 
   Size:        276    1806
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI     AleI     AlfI    AlwNI     ApaI
    ApaLI     ApoI     AvaI     BaeI    BamHI     BanI    BanII     BbsI
     BglI Bme1580I     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI
    BsaHI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI    BsiWI     BsmI
 Bsp1286I    BspMI    BsrDI    BsrFI    BsrGI    BstXI    BstYI   Bsu36I
     BtgI    BtgZI     ClaI     DrdI     EaeI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI     FalI    HaeII    Hin4I     KpnI
     MmeI     MscI     MslI   MspA1I     NcoI     PasI    PflMI     PfoI
     PmlI    PpuMI    PshAI   PspOMI     PstI    PvuII     SacI    SanDI
     SapI     ScaI     SfcI    SgrAI     SmlI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI

 Enzymes that do not cut: 

    AatII     AclI     AfeI    AflII   AflIII     AgeI     AhdI     AloI
     AscI     AseI    AsiSI    AvrII     BbeI    BbvCI     BcgI    BciVI
     BclI    BfrBI    BglII     BlpI    BmgBI     BmtI   Bpu10I    BsaBI
    BsaWI    BsiEI    BsmBI    BspEI    BspHI    BsrBI   BssHII    BssSI
   BstAPI    BstBI   BstEII  BstZ17I     BtsI    CspCI     DraI   DraIII
     EagI    EcoRV     FseI     FspI    FspAI   HincII  HindIII     HpaI
     KasI     MfeI     MluI     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PciI     PmeI     PpiI
     PsiI    PspXI     PsrI     PvuI    RsrII    SacII     SalI     SbfI
    SexAI     SfiI     SfoI     SgfI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI     XhoI     XmaI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com