Restriction map for
pF1KE0385
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# NO CUT ENZYMES ( 111 enzymes) #
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AatII AccII AccIII AclI AflII
AgeI Alw44I AocI ApaI ApaLI
AscI Asp700I AspI AvaI AviII
BalI BanII BbeI BbrPI BbvCI
BcgI BfrI BlpI Bpu1102I BsaWI
BseAI BsgI BsiWI BsmBI BsoBI
Bsp1407I BspEI BsrBI BsrGI BssHII
BssSI Bst1107I BstAPI BstEII BstPI
BstUI BstZ17I Bsu36I CelII CpoI
DraII DraIII DrdI EarI Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
FspI KasI Ksp632I KspI MfeI
MluI MluNI MroI MscI MspA1I
MunI MvnI NarI NheI NotI
NruI PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PstI PvuI PvuII
RsrII SacI SacII SapI SbfI
SexAI SfcI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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Acc65I 1114 GGTACC G^GTACC
AccI 685 GTCGAC GT^[AC][GT]AC
AcyI 2045 GGCGTC G[GA]^CG[CT]C
AhdI 1317 GACCAATAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 296 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 76 AGCGCT AGC^GCT
Asp718I 1114 GGTACC G^GTACC
AspEI 1317 GACCAATAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 1198 CCTAGG C^CTAGG
BamHI 913 GGATCC G^GATCC
BanI 1114 GGTACC G^G[CT][GA]CC
BbsI 707 GTCTTC GAAGAC(2/6)
BciVI 1822 GTATCC GTATCC(6/5)
BclI 1674 TGATCA T^GATCA
BcnI 37 CCCGG CC^[GC]GG
BglI 35 GCCCCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1253 AGATCT A^GATCT
BlnI 1198 CCTAGG C^CTAGG
BpuAI 707 GTCTTC GAAGAC(2/6)
BsaAI 859 TACGTG [CT]AC^GT[GA]
BsaBI 2015 GATTAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2045 GGCGTC G[GA]^CG[CT]C
BsaI 2030 GAGACC GGTCTC(1/5)
BsiEI 1443 CGGCCG CG[GA][CT]^CG
BsmI 1755 GAATGC GAATGC(1/-1)
BspDI 1191 ATCGAT AT^CGAT
BspMI 2124 GCAGGT ACCTGC(4/8)
BsrDI 582 CATTGC GCAATG(2/0)
BstBI 142 TTCGAA TT^CGAA
CfoI 77 GCGC GCG^C
ClaI 1191 ATCGAT AT^CGAT
DraI 286 TTTAAA TTT^AAA
EagI 1443 CGGCCG C^GGCCG
Eam1105I 1317 GACCAATAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1443 CGGCCG C^GGCCG
Eco47III 76 AGCGCT AGC^GCT
Eco52I 1443 CGGCCG C^GGCCG
EcoT22I 1696 ATGCAT ATGCA^T
FbaI 1674 TGATCA T^GATCA
FseI 30 GGCCGGCC GGCCGG^CC
HaeII 76 AGCGCT [GA]GCGC^[CT]
HgaI 2046 GCGTC GACGC(5/10)
HhaI 77 GCGC GCG^C
Hin1I 2045 GGCGTC G[GA]^CG[CT]C
HinP1I 77 GCGC G^CGC
HindIII 713 AAGCTT A^AGCTT
HpaI 1563 GTTAAC GTT^AAC
KpnI 1114 GGTACC GGTAC^C
MamI 2015 GATTAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 31 GCCGGC GCC^GGC
NciI 37 CCCGG CC^[GC]GG
NgoMIV 31 GCCGGC G^CCGGC
NsiI 1696 ATGCAT ATGCA^T
NspV 142 TTCGAA TT^CGAA
SalI 685 GTCGAC G^TCGAC
SfuI 142 TTCGAA TT^CGAA
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# TWO CUT ENZYMES ( 12 enzymes) #
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AvaII 349 GGACC G^G[AT]CC
436 GGACC
BsmAI 2030 GAGAC GTCTC(1/5)
1573 GTCTC
BspLU11I 1559 ACATGT A^CATGT
1782 ACATGT
BsrFI 31 GCCGGC [GA]^CCGG[CT]
60 GCCGGT
Cfr10I 31 GCCGGC [GA]^CCGG[CT]
60 GCCGGT
EaeI 29 TGGCCG [CT]^GGCC[GA]
1443 CGGCCG
FauI 17 GCGGG CCCGC(4/6)
1741 GCGGG
HincII 685 GTCGAC GT[CT]^[GA]AC
1563 GTTAAC
HindII 685 GTCGAC GT[CT]^[GA]AC
1563 GTTAAC
ScaI 630 AGTACT AGT^ACT
2215 AGTACT
SmlI 1231 CTTGAG C^T[CT][GA]AG
1652 CTCAAG
SspI 282 AATATT AAT^ATT
1896 AATATT
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# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AseI AsnI AspHI
BbvI BfaI BmyI BpmI BsaJI
BseRI BsiHKAI BsiYI BslI BsmFI
Bsp1286I BspHI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I Cfr13I
DdeI DpnII DsaI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspI MvaI MwoI
NcoI NdeI NdeII NlaIII NlaIV
NspI PleI PshBI RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII