Restriction map for pF1KE1986
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# NO CUT ENZYMES (  118 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AgeI        
AhdI        Alw44I      ApaI        ApaLI       AscI        
AseI        AsnI        AspEI       AvaI        AviII       
AvrII       BalI        BamHI       BbeI        BbrPI       
BcgI        BclI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BsaWI       BseAI       BsiWI       
BsmBI       BsmI        BsoBI       BspDI       BspEI       
BspHI       BsrBI       BsrDI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstYI       
BstZ17I     CelII       ClaI        CpoI        DraII       
DrdI        EaeI        EagI        Eam1105I    EclXI       
Eco52I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRV       FbaI        FseI        FspI        HincII      
HindII      HpaI        KasI        MfeI        MflI        
MluI        MluNI       MroI        MscI        MunI        
NaeI        NarI        NgoMIV      NheI        NotI        
NruI        NspV        PacI        PaeR7I      PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        PvuII       RcaI        
RsrII       SacI        SalI        SapI        SbfI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        Sse8387I    SspI        
StuI        SwaI        XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        


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# ONE CUT ENZYMES (   62 enzymes) #
###################################

Acc65I       1087  GGTACC               G^GTACC
AccII          57  CGCG                 CG^CG
AcyI            3  GGCGTC               G[GA]^CG[CT]C
AflII         981  CTTAAG               C^TTAAG
AflIII        860  ACATGT               A^C[GA][CT]GT
AlwI           52  GATCC                GGATC(4/5)
AocI          176  CCTCAGG              CC^T[ACGT]AGG
Aor51HI      1289  AGCGCT               AGC^GCT
Asp700I      1144  GAAAGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      1087  GGTACC               G^GTACC
AspHI        1210  GAGCAC               G[AT]GC[AT]^C
AspI         1243  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaII          60  GGTCC                G^G[AT]CC
BbvCI          80  CCTCAGC              CCTCAGC(-5/-2)
BfrI          981  CTTAAG               C^TTAAG
BsaAI         304  TACGTG               [CT]AC^GT[GA]
BsaBI         942  GATGTCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI           3  GGCGTC               G[GA]^CG[CT]C
BseRI          12  CTCCTC               GAGGAG(10/8)
BsgI          658  GTGCAG               GTGCAG(16/14)
BsiEI          32  CGGTCG               CG[GA][CT]^CG
BsiHKAI      1210  GAGCAC               G[AT]GC[AT]^C
BsmFI          61  GTCCC                GGGAC(10/14)
Bsp1407I      863  TGTACA               T^GTACA
BspLU11I      860  ACATGT               A^CATGT
BspMI         664  GCAGGT               ACCTGC(4/8)
BsrFI        1020  ACCGGC               [GA]^CCGG[CT]
BsrGI         863  TGTACA               T^GTACA
BstUI          57  CGCG                 CG^CG
Bsu36I        176  CCTCAGG              CC^T[ACGT]AGG
CfoI         1290  GCGC                 GCG^C
Cfr10I       1020  ACCGGC               [GA]^CCGG[CT]
DraI          805  TTTAAA               TTT^AAA
DraIII        247  CACACTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EarI          794  CTCTTC               CTCTTC(1/4)
Eco47III     1289  AGCGCT               AGC^GCT
Eco81I        176  CCTCAGG              CC^T[ACGT]AGG
EcoT22I       724  ATGCAT               ATGCA^T
HaeII        1289  AGCGCT               [GA]GCGC^[CT]
HgaI            4  GCGTC                GACGC(5/10)
HhaI         1290  GCGC                 GCG^C
Hin1I           3  GGCGTC               G[GA]^CG[CT]C
HinP1I       1290  GCGC                 G^CGC
HindIII      1618  AAGCTT               A^AGCTT
KpnI         1087  GGTACC               GGTAC^C
Ksp632I       794  CTCTTC               CTCTTC(1/4)
KspI           56  CCGCGG               CCGC^GG
MaeII         305  ACGT                 A^CGT
MamI          942  GATGTCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI           57  CGCG                 CG^CG
NdeI         1108  CATATG               CA^TATG
NsiI          724  ATGCAT               ATGCA^T
PflFI        1243  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         246  CCACACTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacII          56  CCGCGG               CCGC^GG
ScaI          998  AGTACT               AGT^ACT
SmlI          981  CTTAAG               C^T[CT][GA]AG
SphI          137  GCATGC               GCATG^C
SspBI         863  TGTACA               T^GTACA
Tth111I      1243  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        246  CCACACTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI         1144  GAAAGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AcsI          568  GAATTT               [GA]^AATT[CT]
              637  AAATTC              
AlwNI          86  CAGCATCTG            CAG[ACGT][ACGT][ACGT]^CTG
              577  CAGAAGCTG           
ApoI          568  GAATTT               [GA]^AATT[CT]
              637  AAATTC              
BanII         976  GAGCCC               G[GA]GC[CT]^C
             1536  GGGCTC              
BbsI         1016  GAAGAC               GAAGAC(2/6)
               27  GTCTTC              
BciVI         592  GGATAC               GTATCC(6/5)
              428  GTATCC              
BpmI         1386  CTCCAG               CTGGAG(16/14)
              491  CTGGAG              
BpuAI        1016  GAAGAC               GAAGAC(2/6)
               27  GTCTTC              
BsaI          102  GAGACC               GGTCTC(1/5)
             1422  GGTCTC              
BstXI        1046  CCACAGCCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1107  CCATATGGGTGG        
FauI           55  CCCGC                CCCGC(4/6)
              765  GCGGG               
MaeIII        893  GTGAC                ^GT[ACGT]AC
             1447  GTGAC               
MspA1I         56  CCGCGG               C[AC]G^C[GT]G
              469  CCGCTG              
NspI          137  GCATGC               [GA]CATG^[CT]
              860  ACATGT              
PleI         1509  GACTC                GAGTC(4/5)
             1633  GAGTC               
TaqI         1014  TCGA                 T^CGA
             1077  TCGA                
Tsp45I        893  GTGAC                ^GT[GC]AC
             1447  GTGAC               
Tsp509I       569  AATT                 ^AATT
              638  AATT                
TthHB8I      1014  TCGA                 T^CGA
             1077  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   59 enzymes) #
#########################################

AciI        AfaI        AluI        BanI        BbvI        
BcnI        BfaI        BmyI        BsaJI       BsiYI       
BslI        BsmAI       Bsp1286I    BsrI        BssKI       
BstAPI      BstF5I      BstNI       Cac8I       Cfr13I      
DdeI        DpnII       DsaI        EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HapII       HinfI       
HpaII       HphI        ItaI        MaeI        MboI        
MboII       MnlI        MseI        MslI        MspI        
MvaI        MwoI        NciI        NcoI        NdeII       
NlaIII      NlaIV       PstI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        StyI        
TfiI        Tru9I       TseI        TspRI       
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