Restriction map for pF1KE1986
 (Linear) (Six-base) MAPSORT of: KIEE1986.seq  Check: 5272  from: 1  to: 1650



KIEE1986.orf

 With 182 enzymes: SgfI * 

                        July 16, 2019 14:21  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     655    1650 
   Size:        655     995
 
Acc65I G'GTAC_C

Cuts at:      0    1087    1650 
   Size:       1087     563
 
AfeI AGC'GCT

Cuts at:      0    1291    1650 
   Size:       1291     359
 
AflII C'TTAA_G

Cuts at:      0     981    1650 
   Size:        981     669
 
AflIII A'CryG_T

Cuts at:      0     860    1650 
   Size:        860     790
 
AlwNI CAG_nnn'CTG

Cuts at:      0      91     582    1650 
   Size:         91     491    1068

  Fragments arranged by size: 

                1068     491      91
 
ApoI r'AATT_y

Cuts at:      0     568     637    1650 
   Size:        568      69    1013

  Fragments arranged by size: 

                1013     568      69
 
BanI G'GyrC_C

Cuts at:      0     120     223    1087    1650 
   Size:        120     103     864     563

  Fragments arranged by size: 

                 864     563     120     103
 
BanII G_rGCy'C

Cuts at:      0     980    1540    1650 
   Size:        980     560     110

  Fragments arranged by size: 

                 980     560     110
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      20    1023    1650 
   Size:         20    1003     627

  Fragments arranged by size: 

                1003     627      20
 
BbvCI CC'TCA_GC

Cuts at:      0      81    1650 
   Size:         81    1569
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     439     586    1650 
   Size:        439     147    1064

  Fragments arranged by size: 

                1064     439     147
 
BfrBI ATG'CAT

Cuts at:      0     726    1650 
   Size:        726     924
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     323    1550    1650 
   Size:        323    1227     100

  Fragments arranged by size: 

                1227     323     100
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     512    1371    1650 
   Size:        512     859     279

  Fragments arranged by size: 

                 859     512     279
 
Bpu10I CC'TnA_GC

Cuts at:      0      81    1650 
   Size:         81    1569
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      96    1428    1650 
   Size:         96    1332     222

  Fragments arranged by size: 

                1332     222      96
 
BsaAI yAC'GTr

Cuts at:      0     306    1650 
   Size:        306    1344
 
BsaBI GATnn'nnATC

Cuts at:      0     946    1650 
   Size:        946     704
 
BsaHI Gr'CG_yC

Cuts at:      0       4    1650 
   Size:          4    1646
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     249     279    1232    1262    1650 
   Size:        249      30     953      30     388

  Fragments arranged by size: 

                 953     388     249      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0       3    1650 
   Size:          3    1647
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     679    1650 
   Size:        679     971
 
BsiEI CG_ry'CG

Cuts at:      0      35    1650 
   Size:         35    1615
 
BsiHKAI G_wGCw'C

Cuts at:      0    1214    1650 
   Size:       1214     436
 
Bsp1286I G_dGCh'C

Cuts at:      0     980    1214    1540    1650 
   Size:        980     234     326     110

  Fragments arranged by size: 

                 980     326     234     110
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     655    1650 
   Size:        655     995
 
BsrFI r'CCGG_y

Cuts at:      0    1020    1650 
   Size:       1020     630
 
BsrGI T'GTAC_A

Cuts at:      0     863    1650 
   Size:        863     787
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     397     582    1400    1650 
   Size:        397     185     818     250

  Fragments arranged by size: 

                 818     397     250     185
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1053    1114    1650 
   Size:       1053      61     536

  Fragments arranged by size: 

                1053     536      61
 
Bsu36I CC'TnA_GG

Cuts at:      0     177    1650 
   Size:        177    1473
 
BtgI C'CryG_G

Cuts at:      0      56     107    1052    1091    1650 
   Size:         56      51     945      39     559

  Fragments arranged by size: 

                 945     559      56      51      39
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1243    1650 
   Size:       1243     407
 
BtsI GCAGTG_nn'

Cuts at:      0     468     993    1650 
   Size:        468     525     657

  Fragments arranged by size: 

                 657     525     468
 
DraI TTT'AAA

Cuts at:      0     807    1650 
   Size:        807     843
 
DraIII CAC_nnn'GTG

Cuts at:      0     252    1650 
   Size:        252    1398
 
EarI CTCTTCn'nnn_

Cuts at:      0     800    1650 
   Size:        800     850
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     580    1650 
   Size:        580    1070
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     512    1371    1650 
   Size:        512     859     279

  Fragments arranged by size: 

                 859     512     279
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1341    1650 
   Size:       1341     309
 
HaeII r_GCGC'y

Cuts at:      0    1293    1650 
   Size:       1293     357
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     339     371     684     716    1232    1234    1264    1266 
   Size:        339      32     313      32     516       2      30       2

Cuts at:   1266    1650 
   Size:        384

  Fragments arranged by size: 

                 516     384     339     313      32      32      30       2
                   2
 
HindIII A'AGCT_T

Cuts at:      0    1618    1650 
   Size:       1618      32
 
KpnI G_GTAC'C

Cuts at:      0    1091    1650 
   Size:       1091     559
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     576    1187    1554    1650 
   Size:        576     611     367      96

  Fragments arranged by size: 

                 611     576     367      96
 
MslI CAynn'nnrTG

Cuts at:      0     164     365    1051    1112    1650 
   Size:        164     201     686      61     538

  Fragments arranged by size: 

                 686     538     201     164      61
 
MspA1I CmG'CkG

Cuts at:      0      58     471    1650 
   Size:         58     413    1179

  Fragments arranged by size: 

                1179     413      58
 
NcoI C'CATG_G

Cuts at:      0     107    1052    1091    1650 
   Size:        107     945      39     559

  Fragments arranged by size: 

                 945     559     107      39
 
NdeI CA'TA_TG

Cuts at:      0    1109    1650 
   Size:       1109     541
 
NsiI A_TGCA'T

Cuts at:      0     728    1650 
   Size:        728     922
 
NspI r_CATG'y

Cuts at:      0     141     864    1650 
   Size:        141     723     786

  Fragments arranged by size: 

                 786     723     141
 
PciI A'CATG_T

Cuts at:      0     860    1650 
   Size:        860     790
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     252    1650 
   Size:        252    1398
 
PfoI T'CCnGG_A

Cuts at:      0     413     588    1650 
   Size:        413     175    1062

  Fragments arranged by size: 

                1062     413     175
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1624    1650 
   Size:       1624      26
 
PstI C_TGCA'G

Cuts at:      0     463     578     890     911    1650 
   Size:        463     115     312      21     739

  Fragments arranged by size: 

                 739     463     312     115      21
 
SacII CC_GC'GG

Cuts at:      0      59    1650 
   Size:         59    1591
 
ScaI AGT'ACT

Cuts at:      0    1000    1650 
   Size:       1000     650
 
SfcI C'TryA_G

Cuts at:      0     459     483     574     886     907    1180    1650 
   Size:        459      24      91     312      21     273     470

  Fragments arranged by size: 

                 470     459     312     273      91      24      21
 
SmlI C'TyrA_G

Cuts at:      0     981    1650 
   Size:        981     669
 
SphI G_CATG'C

Cuts at:      0     141    1650 
   Size:        141    1509
 
StyI C'CwwG_G

Cuts at:      0     107    1052    1091    1482    1610    1650 
   Size:        107     945      39     391     128      40

  Fragments arranged by size: 

                 945     391     128     107      40      39
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      21    1102    1343    1410    1650 
   Size:         21    1081     241      67     240

  Fragments arranged by size: 

                1081     241     240      67      21
 
TatI w'GTAC_w

Cuts at:      0     650     863     998    1603    1650 
   Size:        650     213     135     605      47

  Fragments arranged by size: 

                 650     605     213     135      47
 
Tth111I GACn'n_nGTC

Cuts at:      0    1246    1650 
   Size:       1246     404
 
XmnI GAAnn'nnTTC

Cuts at:      0    1148    1650 
   Size:       1148     502
 

 Enzymes that do cut:

     AarI   Acc65I     AfeI    AflII   AflIII    AlwNI     ApoI     BanI
    BanII     BbsI    BbvCI    BciVI    BfrBI     BmrI     BpmI   Bpu10I
     BsaI    BsaAI    BsaBI    BsaHI    BsaXI    BseRI     BsgI    BsiEI
  BsiHKAI Bsp1286I    BspMI    BsrFI    BsrGI   BstAPI    BstXI   Bsu36I
     BtgI    BtgZI     BtsI     DraI   DraIII     EarI     EciI  Eco57MI
     FalI    HaeII    Hin4I  HindIII     KpnI     MmeI     MslI   MspA1I
     NcoI     NdeI     NsiI     NspI     PciI    PflMI     PfoI     PpiI
     PstI    SacII     ScaI     SfcI     SmlI     SphI     StyI    TaqII
     TatI  Tth111I     XmnI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AcuI     AgeI     AhdI     AleI     AlfI
     AloI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI    BamHI     BbeI     BcgI     BclI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmtI     BplI    BpuEI    BsaWI    BseYI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI   BssHII    BssSI
    BstBI   BstEII    BstYI  BstZ17I     ClaI    CspCI     DrdI     EaeI
     EagI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI
    FspAI   HincII     HpaI     KasI     MfeI     MluI     MscI     NaeI
     NarI   NgoMIV     NheI     NotI     NruI     PacI     PasI     PmeI
     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    PvuII    RsrII     SacI     SalI    SanDI     SapI     SbfI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI
     SspI     StuI     SwaI     TstI     XbaI     XcmI     XhoI     XmaI
     ZraI
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