Restriction map for
pF1KE0356
(Linear) (Six-base) MAPSORT of: KIEE0356.seq Check: 7075 from: 1 to: 1515
KIEE0356.orf
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AclI AA'CG_TT
Cuts at: 0 928 1515
Size: 928 587
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1 255 570 920 938 1515
Size: 1 254 315 350 18 577
Fragments arranged by size:
577 350 315 254 18 1
AflII C'TTAA_G
Cuts at: 0 802 1515
Size: 802 713
AflIII A'CryG_T
Cuts at: 0 543 1515
Size: 543 972
AgeI A'CCGG_T
Cuts at: 0 1131 1515
Size: 1131 384
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 85 1385 1515
Size: 85 1300 130
Fragments arranged by size:
1300 130 85
ApoI r'AATT_y
Cuts at: 0 712 742 1296 1515
Size: 712 30 554 219
Fragments arranged by size:
712 554 219 30
AvrII C'CTAG_G
Cuts at: 0 1041 1515
Size: 1041 474
BamHI G'GATC_C
Cuts at: 0 974 1515
Size: 974 541
BanII G_rGCy'C
Cuts at: 0 8 126 536 1230 1515
Size: 8 118 410 694 285
Fragments arranged by size:
694 410 285 118 8
BbsI GAAGACnn'nnnn_
Cuts at: 0 1046 1262 1515
Size: 1046 216 253
Fragments arranged by size:
1046 253 216
BclI T'GATC_A
Cuts at: 0 116 1515
Size: 116 1399
BglI GCCn_nnn'nGGC
Cuts at: 0 12 596 1515
Size: 12 584 919
Fragments arranged by size:
919 584 12
BglII A'GATC_T
Cuts at: 0 906 1515
Size: 906 609
BmgBI CAC'GTC
Cuts at: 0 526 1515
Size: 526 989
BmrI ACTGGGnnnn_n'
Cuts at: 0 178 1515
Size: 178 1337
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 550 895 1272 1412 1515
Size: 550 345 377 140 103
Fragments arranged by size:
550 377 345 140 103
Bpu10I CC'TnA_GC
Cuts at: 0 652 1001 1075 1515
Size: 652 349 74 440
Fragments arranged by size:
652 440 349 74
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 707 1299 1422 1456 1515
Size: 707 592 123 34 59
Fragments arranged by size:
707 592 123 59 34
BsaBI GATnn'nnATC
Cuts at: 0 1102 1515
Size: 1102 413
BsaWI w'CCGG_w
Cuts at: 0 788 1131 1515
Size: 788 343 384
Fragments arranged by size:
788 384 343
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 46 117 120 1515
Size: 46 71 3 1395
Fragments arranged by size:
1395 71 46 3
BseYI C'CCAG_C
Cuts at: 0 842 1448 1515
Size: 842 606 67
Fragments arranged by size:
842 606 67
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 125 1515
Size: 112 13 1390
Fragments arranged by size:
1390 112 13
BsiHKAI G_wGCw'C
Cuts at: 0 536 1515
Size: 536 979
BsmI GAATG_Cn'
Cuts at: 0 440 1117 1515
Size: 440 677 398
Fragments arranged by size:
677 440 398
Bsp1286I G_dGCh'C
Cuts at: 0 8 126 536 1230 1515
Size: 8 118 410 694 285
Fragments arranged by size:
694 410 285 118 8
BspHI T'CATG_A
Cuts at: 0 572 1515
Size: 572 943
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1203 1515
Size: 1203 312
BsrDI GCAATG_nn'
Cuts at: 0 509 1515
Size: 509 1006
BsrFI r'CCGG_y
Cuts at: 0 1131 1515
Size: 1131 384
BstAPI GCAn_nnn'nTGC
Cuts at: 0 148 1515
Size: 148 1367
BstYI r'GATC_y
Cuts at: 0 318 906 974 1515
Size: 318 588 68 541
Fragments arranged by size:
588 541 318 68
Bsu36I CC'TnA_GG
Cuts at: 0 1262 1515
Size: 1262 253
BtsI GCAGTG_nn'
Cuts at: 0 647 1400 1515
Size: 647 753 115
Fragments arranged by size:
753 647 115
DraIII CAC_nnn'GTG
Cuts at: 0 1100 1515
Size: 1100 415
DrdI GACnn_nn'nnGTC
Cuts at: 0 1264 1515
Size: 1264 251
EaeI y'GGCC_r
Cuts at: 0 165 1515
Size: 165 1350
EarI CTCTTCn'nnn_
Cuts at: 0 1235 1515
Size: 1235 280
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1108 1515
Size: 1108 407
EcoICRI GAG'CTC
Cuts at: 0 534 1515
Size: 534 981
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1 255 550 570 895 920 938 1272
Size: 1 254 295 20 325 25 18 334
Cuts at: 1272 1412 1515
Size: 140 103
Fragments arranged by size:
334 325 295 254 140 103 25 20
18 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 600 1515
Size: 600 915
HaeII r_GCGC'y
Cuts at: 0 843 1515
Size: 843 672
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 101 133 940 972 1013 1045 1171 1203
Size: 101 32 807 32 41 32 126 32
Cuts at: 1203 1515
Size: 312
Fragments arranged by size:
807 312 126 101 41 32 32 32
32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 231 590 689 1515
Size: 231 359 99 826
Fragments arranged by size:
826 359 231 99
MscI TGG'CCA
Cuts at: 0 167 1515
Size: 167 1348
MslI CAynn'nnrTG
Cuts at: 0 460 463 1096 1316 1515
Size: 460 3 633 220 199
Fragments arranged by size:
633 460 220 199 3
MspA1I CmG'CkG
Cuts at: 0 1339 1515
Size: 1339 176
NspI r_CATG'y
Cuts at: 0 66 547 1515
Size: 66 481 968
Fragments arranged by size:
968 481 66
PciI A'CATG_T
Cuts at: 0 543 1515
Size: 543 972
PfoI T'CCnGG_A
Cuts at: 0 782 970 1515
Size: 782 188 545
Fragments arranged by size:
782 545 188
PstI C_TGCA'G
Cuts at: 0 1200 1515
Size: 1200 315
PvuII CAG'CTG
Cuts at: 0 1339 1515
Size: 1339 176
SacI G_AGCT'C
Cuts at: 0 536 1515
Size: 536 979
SapI GCTCTTCn'nnn_
Cuts at: 0 1235 1515
Size: 1235 280
SbfI CC_TGCA'GG
Cuts at: 0 1200 1515
Size: 1200 315
SexAI A'CCwGG_T
Cuts at: 0 701 1515
Size: 701 814
SfcI C'TryA_G
Cuts at: 0 1196 1515
Size: 1196 319
SmlI C'TyrA_G
Cuts at: 0 722 802 1278 1437 1471 1515
Size: 722 80 476 159 34 44
Fragments arranged by size:
722 476 159 80 44 34
SspI AAT'ATT
Cuts at: 0 452 1221 1515
Size: 452 769 294
Fragments arranged by size:
769 452 294
StuI AGG'CCT
Cuts at: 0 1443 1515
Size: 1443 72
StyI C'CwwG_G
Cuts at: 0 1041 1515
Size: 1041 474
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1193 1225 1478 1510 1515
Size: 1193 32 253 32 5
Fragments arranged by size:
1193 253 32 32 5
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 160 1515
Size: 160 1355
XmnI GAAnn'nnTTC
Cuts at: 0 451 928 1515
Size: 451 477 587
Fragments arranged by size:
587 477 451
Enzymes that do cut:
AclI AcuI AflII AflIII AgeI AlfI ApoI AvrII
BamHI BanII BbsI BclI BglI BglII BmgBI BmrI
BpmI Bpu10I BpuEI BsaBI BsaWI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BsrFI BstAPI
BstYI Bsu36I BtsI DraIII DrdI EaeI EarI EciI
EcoICRI Eco57MI FalI HaeII Hin4I MmeI MscI MslI
MspA1I NspI PciI PfoI PstI PvuII SacI SapI
SbfI SexAI SfcI SmlI SspI StuI StyI TstI
XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AhdI AleI AloI
AlwNI ApaI ApaLI AscI AseI AsiSI AvaI BaeI
BanI BbeI BbvCI BcgI BciVI BfrBI BlpI Bme1580I
BmtI BplI BsaI BsaAI BsaHI BsaXI BsiEI BsiWI
BsmBI BspEI BsrBI BsrGI BssHII BssSI BstBI BstEII
BstXI BstZ17I BtgI BtgZI ClaI CspCI DraI EagI
EcoNI EcoO109I EcoRI EcoRV FseI FspI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI
ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SwaI TaqII TatI Tth111I XbaI XhoI
XmaI ZraI