Restriction map for pF1KE2067
##################################
# NO CUT ENZYMES (  110 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AgeI        AhdI        Alw44I      Aor51HI     ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AviII       AvrII       BamHI       
BbeI        BfrI        BglI        BlnI        BlpI        
Bpu1102I    BsaI        BsaWI       BseAI       BsiWI       
BsmI        Bsp1407I    BspDI       BspEI       BspLU11I    
BsrGI       BssHII      BssSI       Bst1107I    BstBI       
BstXI       BstZ17I     CelII       ClaI        CpoI        
DraI        DraIII      EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoRI       EcoRV       EcoT22I     
FseI        FspI        HaeII       HindIII     HpaI        
KasI        KpnI        KspI        MfeI        MluI        
MroI        MseI        MunI        NaeI        NarI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmeI        PpuMI       PshAI       PshBI       
Psp1406I    RsrII       SacI        SacII       SalI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SspBI       
SspI        SwaI        Tru9I       Tth111I     XbaI        
XcmI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AcsI         2122  AAATTC               [GA]^AATT[CT]
AcyI          336  GACGCC               G[GA]^CG[CT]C
AflIII       1142  ACACGT               A^C[GA][CT]GT
AocI          514  CCTAAGG              CC^T[ACGT]AGG
ApoI         2122  AAATTC               [GA]^AATT[CT]
AspHI        1047  GAGCAC               G[AT]GC[AT]^C
BbrPI        1380  CACGTG               CAC^GTG
BbvCI        1983  CCTCAGC              CCTCAGC(-5/-2)
BciVI        1629  GGATAC               GTATCC(6/5)
BfaI         1729  CTAG                 C^TAG
BglII         914  AGATCT               A^GATCT
BsaBI        1428  GATTCGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         336  GACGCC               G[GA]^CG[CT]C
BsiEI         807  CGATCG               CG[GA][CT]^CG
BsiHKAI      1047  GAGCAC               G[AT]GC[AT]^C
BspHI        1103  TCATGA               T^CATGA
BsrBI         388  GAGCGG               CCGCTC(-3/-3)
BsrDI        1569  CATTGC               GCAATG(2/0)
BstEII       2058  GGTCACC              G^GT[ACGT]ACC
BstPI        2058  GGTCACC              G^GT[ACGT]ACC
Bsu36I        514  CCTAAGG              CC^T[ACGT]AGG
DrdI          460  GACACCTATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        514  CCTAAGG              CC^T[ACGT]AGG
EcoO65I      2058  GGTCACC              G^GT[ACGT]ACC
Hin1I         336  GACGCC               G[GA]^CG[CT]C
HincII       1945  GTCAAC               GT[CT]^[GA]AC
HindII       1945  GTCAAC               GT[CT]^[GA]AC
MaeI         1729  CTAG                 C^TAG
MamI         1428  GATTCGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI          867  CCATGG               C^CATGG
PmaCI        1380  CACGTG               CAC^GTG
PmlI         1380  CACGTG               CAC^GTG
PvuI          807  CGATCG               CGAT^CG
RcaI         1103  TCATGA               T^CATGA
SbfI          924  CCTGCAGG             CCTGCA^GG
ScaI         1482  AGTACT               AGT^ACT
Sse8387I      924  CCTGCAGG             CCTGCA^GG


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AccI          418  GTCTAC               GT^[AC][GT]AC
             1593  GTCTAC              
AccII         157  CGCG                 CG^CG
              270  CGCG                
AlwNI         594  CAGCAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1552  CAGCTCCTG           
ApaI          254  GGGCCC               GGGCC^C
              446  GGGCCC              
AvaI           19  CCCGAG               C^[CT]CG[GA]G
               85  CCCGAG              
BalI          281  TGGCCA               TGG^CCA
              377  TGGCCA              
BcgI          700  GCAGCGGTGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1065  GCAGAAGAGTCG        
BclI          506  TGATCA               T^GATCA
              554  TGATCA              
BsaAI        1143  CACGTA               [CT]AC^GT[GA]
             1380  CACGTG              
BsoBI          19  CCCGAG               C^[CT]CG[GA]G
               85  CCCGAG              
BsrFI         690  ACCGGC               [GA]^CCGG[CT]
             1010  ACCGGC              
BstAPI        593  GCAGCAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1780  GCAAAGTCTGC         
BstUI         157  CGCG                 CG^CG
              270  CGCG                
BstYI         848  AGATCC               [GA]^GATC[CT]
              914  AGATCT              
Cfr10I        690  ACCGGC               [GA]^CCGG[CT]
             1010  ACCGGC              
DsaI          867  CCATGG               C^C[GA][CT]GG
             1205  CCACGG              
FbaI          506  TGATCA               T^GATCA
              554  TGATCA              
MflI          848  AGATCC               [GA]^GATC[CT]
              914  AGATCT              
MluNI         281  TGGCCA               TGG^CCA
              377  TGGCCA              
MscI          281  TGGCCA               TGG^CCA
              377  TGGCCA              
MvnI          157  CGCG                 CG^CG
              270  CGCG                
SapI         1578  GAAGAGC              GCTCTTC(1/4)
             1762  GAAGAGC             
SphI         1263  GCATGC               GCATG^C
             1421  GCATGC              
StuI          204  AGGCCT               AGG^CCT
             1992  AGGCCT              
TaqI         1436  TCGA                 T^CGA
             1506  TCGA                
Tsp509I      1451  AATT                 ^AATT
             2123  AATT                
TthHB8I      1436  TCGA                 T^CGA
             1506  TCGA                
XhoII         848  AGATCC               [GA]^GATC[CT]
              914  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   83 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AvaII       
BanI        BanII       BbsI        BbvI        BcnI        
BmyI        BpmI        BpuAI       BsaJI       BseRI       
BsgI        BsiYI       BslI        BsmAI       BsmBI       
BsmFI       Bsp1286I    BspMI       BsrI        BssKI       
BstF5I      BstNI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       EaeI        EarI        
EcoNI       EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HgaI        
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeII       MaeIII      MboI        
MboII       MnlI        MslI        MspA1I      MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        PflMI       PleI        PstI        
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        StyI        TfiI        TseI        
Tsp45I      TspRI       Van91I      
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