Restriction map for pF1KE2067
 (Linear) (Six-base) MAPSORT of: KIEE2067.seq  Check: 9078  from: 1  to: 2178



KIEE2067.orf

 With 182 enzymes: SgfI * 

                        October 24, 2019 20:49  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     689     850     932    1785    1800    2178 
   Size:        689     161      82     853      15     378

  Fragments arranged by size: 

                 853     689     378     161      82      15
 
AccI GT'mk_AC

Cuts at:      0     419    1594    2178 
   Size:        419    1175     584

  Fragments arranged by size: 

                1175     584     419
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1653    2178 
   Size:       1653     525
 
AflIII A'CryG_T

Cuts at:      0    1142    2178 
   Size:       1142    1036
 
AleI CACnn'nnGTG

Cuts at:      0    1184    2178 
   Size:       1184     994
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1438    2178 
   Size:       1438     740
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     226     258    2178 
   Size:        226      32    1920

  Fragments arranged by size: 

                1920     226      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     599    1557    2178 
   Size:        599     958     621

  Fragments arranged by size: 

                 958     621     599
 
ApaI G_GGCC'C

Cuts at:      0     258     450    2178 
   Size:        258     192    1728

  Fragments arranged by size: 

                1728     258     192
 
ApoI r'AATT_y

Cuts at:      0    2122    2178 
   Size:       2122      56
 
AvaI C'yCGr_G

Cuts at:      0      19      85    2178 
   Size:         19      66    2093

  Fragments arranged by size: 

                2093      66      19
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     934     967    2178 
   Size:        934      33    1211

  Fragments arranged by size: 

                1211     934      33
 
BanI G'GyrC_C

Cuts at:      0    1237    1245    1656    2178 
   Size:       1237       8     411     522

  Fragments arranged by size: 

                1237     522     411       8
 
BanII G_rGCy'C

Cuts at:      0     258     450    1030    1813    1840    1916    2089    2178 
   Size:        258     192     580     783      27      76     173      89

  Fragments arranged by size: 

                 783     580     258     192     173      89      76      27
            
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     294    1294    1888    2178 
   Size:        294    1000     594     290

  Fragments arranged by size: 

                1000     594     294     290
 
BbvCI CC'TCA_GC

Cuts at:      0    1984    2178 
   Size:       1984     194
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     689     723    1054    1088    2178 
   Size:        689      34     331      34    1090

  Fragments arranged by size: 

                1090     689     331      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1623    2178 
   Size:       1623     555
 
BclI T'GATC_A

Cuts at:      0     506     554    2178 
   Size:        506      48    1624

  Fragments arranged by size: 

                1624     506      48
 
BglII A'GATC_T

Cuts at:      0     914    2178 
   Size:        914    1264
 
Bme1580I G_kGCm'C

Cuts at:      0     258     450    1242    1250    1661    2178 
   Size:        258     192     792       8     411     517

  Fragments arranged by size: 

                 792     517     411     258     192       8
 
BmgBI CAC'GTC

Cuts at:      0     663    2178 
   Size:        663    1515
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     601    2178 
   Size:        601    1577
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      45    1044    1788    2178 
   Size:         45     999     744     390

  Fragments arranged by size: 

                 999     744     390      45
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1015    1507    1784    2178 
   Size:       1015     492     277     394

  Fragments arranged by size: 

                1015     492     394     277
 
Bpu10I CC'TnA_GC

Cuts at:      0    1984    1996    2178 
   Size:       1984      12     182

  Fragments arranged by size: 

                1984     182      12
 
BsaAI yAC'GTr

Cuts at:      0    1145    1382    2178 
   Size:       1145     237     796

  Fragments arranged by size: 

                1145     796     237
 
BsaBI GATnn'nnATC

Cuts at:      0    1432    2178 
   Size:       1432     746
 
BsaHI Gr'CG_yC

Cuts at:      0     337    2178 
   Size:        337    1841
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     226     256     566     596    1075    1105    2178 
   Size:        226      30     310      30     479      30    1073

  Fragments arranged by size: 

                1073     479     310     226      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     208     231     913    1243    1365    1663    1711    1972 
   Size:        208      23     682     330     122     298      48     261

Cuts at:   1972    1975    2178 
   Size:          3     203

  Fragments arranged by size: 

                 682     330     298     261     208     203     122      48
                  23       3
 
BseYI C'CCAG_C

Cuts at:      0     443     449    1077    1550    1812    1925    2178 
   Size:        443       6     628     473     262     113     253

  Fragments arranged by size: 

                 628     473     443     262     253     113       6
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     616     667    1054    1772    1825    2178 
   Size:        616      51     387     718      53     353

  Fragments arranged by size: 

                 718     616     387     353      53      51
 
BsiEI CG_ry'CG

Cuts at:      0     810    2178 
   Size:        810    1368
 
BsiHKAI G_wGCw'C

Cuts at:      0    1051    2178 
   Size:       1051    1127
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0       4    1616    1645    2178 
   Size:          4    1612      29     533

  Fragments arranged by size: 

                1612     533      29       4
 
Bsp1286I G_dGCh'C

Cuts at:      0     258     450    1030    1051    1242    1250    1661    1813 
   Size:        258     192     580      21     191       8     411     152

Cuts at:   1813    1840    1916    2089    2178 
   Size:         27      76     173      89

  Fragments arranged by size: 

                 580     411     258     192     191     173     152      89
                  76      27      21       8
 
BspHI T'CATG_A

Cuts at:      0    1103    2178 
   Size:       1103    1075
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     128     689     827     850     932    1328    1785    1800 
   Size:        128     561     138      23      82     396     457      15

Cuts at:   1800    2178 
   Size:        378

  Fragments arranged by size: 

                 561     457     396     378     138     128      82      23
                  15
 
BsrBI CCG'CTC

Cuts at:      0     390    2178 
   Size:        390    1788
 
BsrDI GCAATG_nn'

Cuts at:      0    1568    2178 
   Size:       1568     610
 
BsrFI r'CCGG_y

Cuts at:      0     690    1010    2178 
   Size:        690     320    1168

  Fragments arranged by size: 

                1168     690     320
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     599    1786    2178 
   Size:        599    1187     392

  Fragments arranged by size: 

                1187     599     392
 
BstEII G'GTnAC_C

Cuts at:      0    2058    2178 
   Size:       2058     120
 
BstYI r'GATC_y

Cuts at:      0     848     914    2178 
   Size:        848      66    1264

  Fragments arranged by size: 

                1264     848      66
 
Bsu36I CC'TnA_GG

Cuts at:      0     515    2178 
   Size:        515    1663
 
BtgI C'CryG_G

Cuts at:      0     867    1205    2178 
   Size:        867     338     973

  Fragments arranged by size: 

                 973     867     338
 
BtsI GCAGTG_nn'

Cuts at:      0     420     537    1042    1422    2178 
   Size:        420     117     505     380     756

  Fragments arranged by size: 

                 756     505     420     380     117
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     466    2178 
   Size:        466    1712
 
EaeI y'GGCC_r

Cuts at:      0     281     377     638     692     710    1334    2178 
   Size:        281      96     261      54      18     624     844

  Fragments arranged by size: 

                 844     624     281     261      96      54      18
 
EarI CTCTTCn'nnn_

Cuts at:      0       2    1063    1573    1757    2178 
   Size:          2    1061     510     184     421

  Fragments arranged by size: 

                1061     510     421     184       2
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     130    2178 
   Size:        130    2048
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1015    1507    1653    1784    2178 
   Size:       1015     492     146     131     394

  Fragments arranged by size: 

                1015     492     394     146     131
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     124     186     211     556     997    2178 
   Size:        124      62      25     345     441    1181

  Fragments arranged by size: 

                1181     441     345     124      62      25
 
EcoO109I rG'GnC_Cy

Cuts at:      0     255     723    1098    1540    2178 
   Size:        255     468     375     442     638

  Fragments arranged by size: 

                 638     468     442     375     255
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      55      87    1516    1548    2178 
   Size:         55      32    1429      32     630

  Fragments arranged by size: 

                1429     630      55      32      32
 
HincII GTy'rAC

Cuts at:      0    1947    2178 
   Size:       1947     231
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     269    1673    2178 
   Size:        269    1404     505

  Fragments arranged by size: 

                1404     505     269
 
MscI TGG'CCA

Cuts at:      0     283     379    2178 
   Size:        283      96    1799

  Fragments arranged by size: 

                1799     283      96
 
MslI CAynn'nnrTG

Cuts at:      0    1108    1184    1384    2178 
   Size:       1108      76     200     794

  Fragments arranged by size: 

                1108     794     200      76
 
MspA1I CmG'CkG

Cuts at:      0     361     453     599     703    1295    1537    1608    2066 
   Size:        361      92     146     104     592     242      71     458

Cuts at:   2066    2178 
   Size:        112

  Fragments arranged by size: 

                 592     458     361     242     146     112     104      92
                  71
 
NcoI C'CATG_G

Cuts at:      0     867    2178 
   Size:        867    1311
 
NspI r_CATG'y

Cuts at:      0      96     938    1267    1425    2178 
   Size:         96     842     329     158     753

  Fragments arranged by size: 

                 842     753     329     158      96
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     251     719     873    1023    1557    1692    2178 
   Size:        251     468     154     150     534     135     486

  Fragments arranged by size: 

                 534     486     468     251     154     150     135
 
PfoI T'CCnGG_A

Cuts at:      0     212     428     992    2178 
   Size:        212     216     564    1186

  Fragments arranged by size: 

                1186     564     216     212
 
PmlI CAC'GTG

Cuts at:      0    1382    2178 
   Size:       1382     796
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     226     258    2178 
   Size:        226      32    1920

  Fragments arranged by size: 

                1920     226      32
 
PspOMI G'GGCC_C

Cuts at:      0     254     446    2178 
   Size:        254     192    1732

  Fragments arranged by size: 

                1732     254     192
 
PstI C_TGCA'G

Cuts at:      0     176     824     929    2178 
   Size:        176     648     105    1249

  Fragments arranged by size: 

                1249     648     176     105
 
PvuI CG_AT'CG

Cuts at:      0     810    2178 
   Size:        810    1368
 
PvuII CAG'CTG

Cuts at:      0     361     453     599    1608    2178 
   Size:        361      92     146    1009     570

  Fragments arranged by size: 

                1009     570     361     146      92
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1573    1757    2178 
   Size:       1573     184     421

  Fragments arranged by size: 

                1573     421     184
 
SbfI CC_TGCA'GG

Cuts at:      0     929    2178 
   Size:        929    1249
 
ScaI AGT'ACT

Cuts at:      0    1484    2178 
   Size:       1484     694
 
SfcI C'TryA_G

Cuts at:      0     172     820     925    1128    1595    2178 
   Size:        172     648     105     203     467     583

  Fragments arranged by size: 

                 648     583     467     203     172     105
 
SphI G_CATG'C

Cuts at:      0    1267    1425    2178 
   Size:       1267     158     753

  Fragments arranged by size: 

                1267     753     158
 
StuI AGG'CCT

Cuts at:      0     206    1994    2178 
   Size:        206    1788     184

  Fragments arranged by size: 

                1788     206     184
 
StyI C'CwwG_G

Cuts at:      0     831     867    1616    2178 
   Size:        831      36     749     562

  Fragments arranged by size: 

                 831     749     562      36
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     874    2046    2178 
   Size:        874    1172     132

  Fragments arranged by size: 

                1172     874     132
 
TatI w'GTAC_w

Cuts at:      0      89    1482    1700    2178 
   Size:         89    1393     218     478

  Fragments arranged by size: 

                1393     478     218      89
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     566     598    2178 
   Size:        566      32    1580

  Fragments arranged by size: 

                1580     566      32
 

 Enzymes that do cut:

     AarI     AccI     AcuI   AflIII     AleI     AlfI     AloI    AlwNI
     ApaI     ApoI     AvaI     BaeI     BanI    BanII     BbsI    BbvCI
     BcgI    BciVI     BclI    BglII Bme1580I    BmgBI     BmrI     BplI
     BpmI   Bpu10I    BsaAI    BsaBI    BsaHI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspHI    BspMI    BsrBI
    BsrDI    BsrFI   BstAPI   BstEII    BstYI   Bsu36I     BtgI     BtsI
     DrdI     EaeI     EarI     EciI  Eco57MI    EcoNI EcoO109I    Hin4I
   HincII     MmeI     MscI     MslI   MspA1I     NcoI     NspI    PflMI
     PfoI     PmlI     PpiI   PspOMI     PstI     PvuI    PvuII     SapI
     SbfI     ScaI     SfcI     SphI     StuI     StyI    TaqII     TatI
     TstI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI    ApaLI
     AscI     AseI    AsiSI    AvrII    BamHI     BbeI    BfrBI     BglI
     BlpI     BmtI    BpuEI     BsaI    BsaWI    BsiWI     BsmI    BspEI
    BsrGI   BssHII    BssSI    BstBI    BstXI  BstZ17I    BtgZI     ClaI
    CspCI     DraI   DraIII     EagI  EcoICRI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI     PmeI    PpuMI    PshAI
     PsiI    PspXI     PsrI    RsrII     SacI    SacII     SalI    SanDI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SrfI     SspI     SwaI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI
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