Restriction map for pF1KE0333
##################################
# NO CUT ENZYMES (  135 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      Aor51HI     ApaI        ApaLI       AscI        
Asp718I     AspEI       AspI        AvaI        AviII       
AvrII       BanI        BbeI        BbrPI       BbvCI       
BcgI        BciVI       BclI        BcnI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsaHI       BsaI        BsaWI       BseAI       
BsiWI       BsmAI       BsmBI       BsoBI       Bsp1407I    
BspDI       BspEI       BspLU11I    BspMI       BsrBI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstUI       BstZ17I     CelII       CfoI        
ClaI        CpoI        DraIII      DrdI        Eam1105I    
Eco47III    EcoRV       EcoT22I     FauI        FbaI        
FseI        FspI        HaeII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HpaI        KasI        KpnI        KspI        MfeI        
MluI        MroI        MunI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       Psp1406I    
PvuI        RsrII       SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
Sse8387I    SspBI       SwaI        TaqI        Tth111I     
TthHB8I     XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AccI          379  GTCTAC               GT^[AC][GT]AC
AocI          840  CCTCAGG              CC^T[ACGT]AGG
AseI         1839  ATTAAT               AT^TAAT
AsnI         1839  ATTAAT               AT^TAAT
BalI          122  TGGCCA               TGG^CCA
BamHI         771  GGATCC               G^GATCC
BbsI         1375  GTCTTC               GAAGAC(2/6)
BfaI          736  CTAG                 C^TAG
BpuAI        1375  GTCTTC               GAAGAC(2/6)
BsaBI        1120  GATGAAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI        1705  GAGGAG               GAGGAG(10/8)
BsiEI         974  CGGCCG               CG[GA][CT]^CG
BsmFI        1245  GGGAC                GGGAC(10/14)
BspHI        1680  TCATGA               T^CATGA
BsrDI        1089  CATTGC               GCAATG(2/0)
BsrFI         710  ACCGGC               [GA]^CCGG[CT]
BstXI          14  CCAAAATGTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        840  CCTCAGG              CC^T[ACGT]AGG
Cfr10I        710  ACCGGC               [GA]^CCGG[CT]
DraII         410  AGGCCCT              [GA]G^G[ACGT]CC[CT]
DsaI          206  CCACGG               C^C[GA][CT]GG
EagI          974  CGGCCG               C^GGCCG
EarI         1195  GAAGAG               CTCTTC(1/4)
EclXI         974  CGGCCG               C^GGCCG
Eco52I        974  CGGCCG               C^GGCCG
Eco81I        840  CCTCAGG              CC^T[ACGT]AGG
EcoO109I      410  AGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoRI        1132  GAATTC               G^AATTC
HapII         711  CCGG                 C^CGG
HpaII         711  CCGG                 C^CGG
Ksp632I      1195  GAAGAG               CTCTTC(1/4)
MaeI          736  CTAG                 C^TAG
MamI         1120  GATGAAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         122  TGGCCA               TGG^CCA
MscI          122  TGGCCA               TGG^CCA
MslI          231  CATCTGGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI          711  CCGG                 C^CGG
NspI         1065  GCATGC               [GA]CATG^[CT]
PshBI        1839  ATTAAT               AT^TAAT
RcaI         1680  TCATGA               T^CATGA
SmlI         1553  CTCAAG               C^T[CT][GA]AG
SphI         1065  GCATGC               GCATG^C
StuI          475  AGGCCT               AGG^CCT
XcmI          520  CCAGACTTTCTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AciI          973  GCGG                 CCGC(-3/-1)
             1069  GCGG                
AcsI          778  AAATTC               [GA]^AATT[CT]
             1132  GAATTC              
ApoI          778  AAATTC               [GA]^AATT[CT]
             1132  GAATTC              
Asp700I       693  GAAGTGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1140  GAAGAAGTTC          
BsmI          603  GAATGC               GAATGC(1/-1)
             1713  GAATGC              
BstEII        319  GGTGACC              G^GT[ACGT]ACC
              571  GGTCACC             
BstPI         319  GGTGACC              G^GT[ACGT]ACC
              571  GGTCACC             
BstYI         341  GGATCT               [GA]^GATC[CT]
              771  GGATCC              
EcoO65I       319  GGTGACC              G^GT[ACGT]ACC
              571  GGTCACC             
MaeII        1061  ACGT                 A^CGT
             1737  ACGT                
MflI          341  GGATCT               [GA]^GATC[CT]
              771  GGATCC              
MwoI         1044  GCTCCAGGAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
             1056  GCTGGACGTGC         
PstI         1186  CTGCAG               CTGCA^G
             1600  CTGCAG              
PvuII         421  CAGCTG               CAG^CTG
             1490  CAGCTG              
SspI          742  AATATT               AAT^ATT
             1818  AATATT              
XhoII         341  GGATCT               [GA]^GATC[CT]
              771  GGATCC              
XmnI          693  GAAGTGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1140  GAAGAAGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   62 enzymes) #
#########################################

AfaI        AluI        AlwI        AlwNI       AspHI       
AvaII       BanII       BbvI        BmyI        BpmI        
BsaJI       BsgI        BsiHKAI     BsiYI       BslI        
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
Cac8I       Cfr13I      DdeI        DpnII       DraI        
EaeI        EcoNI       EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HinfI       HphI        ItaI        
MaeIII      MboI        MboII       MnlI        MseI        
MspA1I      MvaI        NdeII       NlaIII      NlaIV       
PflMI       PleI        RsaI        SacI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        StyI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       Van91I      
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