Restriction map for pF1KE0333
 (Linear) (Six-base) MAPSORT of: KIEE0333.seq  Check: 9056  from: 1  to: 1899



KIEE0333.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:13  ..

AccI GT'mk_AC

Cuts at:      0     380    1899 
   Size:        380    1519
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     580    1609    1899 
   Size:        580    1029     290

  Fragments arranged by size: 

                1029     580     290
 
AlwNI CAG_nnn'CTG

Cuts at:      0     414     862    1053    1899 
   Size:        414     448     191     846

  Fragments arranged by size: 

                 846     448     414     191
 
ApoI r'AATT_y

Cuts at:      0     778    1132    1899 
   Size:        778     354     767

  Fragments arranged by size: 

                 778     767     354
 
AseI AT'TA_AT

Cuts at:      0    1840    1899 
   Size:       1840      59
 
BamHI G'GATC_C

Cuts at:      0     771    1899 
   Size:        771    1128
 
BanII G_rGCy'C

Cuts at:      0     205     443    1238    1870    1899 
   Size:        205     238     795     632      29

  Fragments arranged by size: 

                 795     632     238     205      29
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1368    1899 
   Size:       1368     531
 
Bme1580I G_kGCm'C

Cuts at:      0     251    1295    1899 
   Size:        251    1044     604

  Fragments arranged by size: 

                1044     604     251
 
BmgBI CAC'GTC

Cuts at:      0    1062    1899 
   Size:       1062     837
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     872    1899 
   Size:        872    1027
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     436     574     619     952    1030    1307    1411    1899 
   Size:        436     138      45     333      78     277     104     488

  Fragments arranged by size: 

                 488     436     333     277     138     104      78      45
            
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1538    1899 
   Size:       1538     361
 
BsaBI GATnn'nnATC

Cuts at:      0    1124    1899 
   Size:       1124     775
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     547     577    1899 
   Size:        547      30    1322

  Fragments arranged by size: 

                1322     547      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1720    1899 
   Size:       1720     179
 
BseYI C'CCAG_C

Cuts at:      0     956    1016    1899 
   Size:        956      60     883

  Fragments arranged by size: 

                 956     883      60
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     232     387    1000    1899 
   Size:        232     155     613     899

  Fragments arranged by size: 

                 899     613     232     155
 
BsiEI CG_ry'CG

Cuts at:      0     977    1899 
   Size:        977     922
 
BsiHKAI G_wGCw'C

Cuts at:      0     362     397     443    1109    1238    1652    1870    1899 
   Size:        362      35      46     666     129     414     218      29

  Fragments arranged by size: 

                 666     414     362     218     129      46      35      29
            
 
BsmI GAATG_Cn'

Cuts at:      0     609    1719    1899 
   Size:        609    1110     180

  Fragments arranged by size: 

                1110     609     180
 
Bsp1286I G_dGCh'C

Cuts at:      0     205     251     362     397     443    1109    1238    1295 
   Size:        205      46     111      35      46     666     129      57

Cuts at:   1295    1652    1870    1899 
   Size:        357     218      29

  Fragments arranged by size: 

                 666     357     218     205     129     111      57      46
                  46      35      29
 
BspHI T'CATG_A

Cuts at:      0    1680    1899 
   Size:       1680     219
 
BsrDI GCAATG_nn'

Cuts at:      0    1088    1899 
   Size:       1088     811
 
BsrFI r'CCGG_y

Cuts at:      0     710    1899 
   Size:        710    1189
 
BstEII G'GTnAC_C

Cuts at:      0     319     571    1899 
   Size:        319     252    1328

  Fragments arranged by size: 

                1328     319     252
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      21    1899 
   Size:         21    1878
 
BstYI r'GATC_y

Cuts at:      0     341     771    1899 
   Size:        341     430    1128

  Fragments arranged by size: 

                1128     430     341
 
Bsu36I CC'TnA_GG

Cuts at:      0     841    1899 
   Size:        841    1058
 
BtgI C'CryG_G

Cuts at:      0     206    1899 
   Size:        206    1693
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     320    1899 
   Size:        320    1579
 
BtsI GCAGTG_nn'

Cuts at:      0     893     926     947    1472    1899 
   Size:        893      33      21     525     427

  Fragments arranged by size: 

                 893     525     427      33      21
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     486     521    1899 
   Size:        486      35    1378

  Fragments arranged by size: 

                1378     486      35
 
DraI TTT'AAA

Cuts at:      0    1514    1734    1878    1899 
   Size:       1514     220     144      21

  Fragments arranged by size: 

                1514     220     144      21
 
EaeI y'GGCC_r

Cuts at:      0     122     712     974    1899 
   Size:        122     590     262     925

  Fragments arranged by size: 

                 925     590     262     122
 
EagI C'GGCC_G

Cuts at:      0     974    1899 
   Size:        974     925
 
EarI CTCTTCn'nnn_

Cuts at:      0    1190    1899 
   Size:       1190     709
 
EcoICRI GAG'CTC

Cuts at:      0     441    1236    1868    1899 
   Size:        441     795     632      31

  Fragments arranged by size: 

                 795     632     441      31
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     436     574     580     619     952    1030    1307    1411 
   Size:        436     138       6      39     333      78     277     104

Cuts at:   1411    1609    1899 
   Size:        198     290

  Fragments arranged by size: 

                 436     333     290     277     198     138     104      78
                  39       6
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     471     844    1077    1899 
   Size:        471     373     233     822

  Fragments arranged by size: 

                 822     471     373     233
 
EcoO109I rG'GnC_Cy

Cuts at:      0     411    1899 
   Size:        411    1488
 
EcoRI G'AATT_C

Cuts at:      0    1132    1899 
   Size:       1132     767
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     153     185     739     771    1536    1568    1899 
   Size:        153      32     554      32     765      32     331

  Fragments arranged by size: 

                 765     554     331     153      32      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1625    1899 
   Size:       1625     274
 
MscI TGG'CCA

Cuts at:      0     124    1899 
   Size:        124    1775
 
MslI CAynn'nnrTG

Cuts at:      0     235    1899 
   Size:        235    1664
 
MspA1I CmG'CkG

Cuts at:      0     423     973    1492    1899 
   Size:        423     550     519     407

  Fragments arranged by size: 

                 550     519     423     407
 
NspI r_CATG'y

Cuts at:      0    1069    1899 
   Size:       1069     830
 
PasI CC'CwG_GG

Cuts at:      0     659    1899 
   Size:        659    1240
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     276     414    1011    1899 
   Size:        276     138     597     888

  Fragments arranged by size: 

                 888     597     276     138
 
PfoI T'CCnGG_A

Cuts at:      0     337     782    1046    1284    1388    1899 
   Size:        337     445     264     238     104     511

  Fragments arranged by size: 

                 511     445     337     264     238     104
 
PstI C_TGCA'G

Cuts at:      0    1190    1604    1899 
   Size:       1190     414     295

  Fragments arranged by size: 

                1190     414     295
 
PvuII CAG'CTG

Cuts at:      0     423    1492    1899 
   Size:        423    1069     407

  Fragments arranged by size: 

                1069     423     407
 
SacI G_AGCT'C

Cuts at:      0     443    1238    1870    1899 
   Size:        443     795     632      29

  Fragments arranged by size: 

                 795     632     443      29
 
SfcI C'TryA_G

Cuts at:      0     999    1186    1600    1899 
   Size:        999     187     414     299

  Fragments arranged by size: 

                 999     414     299     187
 
SmlI C'TyrA_G

Cuts at:      0    1553    1899 
   Size:       1553     346
 
SphI G_CATG'C

Cuts at:      0    1069    1899 
   Size:       1069     830
 
SspI AAT'ATT

Cuts at:      0     744    1820    1899 
   Size:        744    1076      79

  Fragments arranged by size: 

                1076     744      79
 
StuI AGG'CCT

Cuts at:      0     477    1899 
   Size:        477    1422
 
StyI C'CwwG_G

Cuts at:      0     224     472    1787    1899 
   Size:        224     248    1315     112

  Fragments arranged by size: 

                1315     248     224     112
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     269    1899 
   Size:        269    1630
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     527    1899 
   Size:        527    1372
 
XmnI GAAnn'nnTTC

Cuts at:      0     697    1144    1899 
   Size:        697     447     755

  Fragments arranged by size: 

                 755     697     447
 

 Enzymes that do cut:

     AccI     AcuI    AlwNI     ApoI     AseI    BamHI    BanII     BbsI
 Bme1580I    BmgBI     BmrI     BpmI    BpuEI    BsaBI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspHI    BsrDI
    BsrFI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI
    CspCI     DraI     EaeI     EagI     EarI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI    Hin4I     MmeI     MscI     MslI   MspA1I     NspI
     PasI    PflMI     PfoI     PstI    PvuII     SacI     SfcI     SmlI
     SphI     SspI     StuI     StyI    TaqII     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI    AsiSI
     AvaI    AvrII     BaeI     BanI     BbeI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI     BmtI     BplI   Bpu10I
     BsaI    BsaAI    BsaHI    BsaWI    BsiWI    BsmBI    BspEI    BspMI
    BsrBI    BsrGI   BssHII    BssSI   BstAPI    BstBI  BstZ17I     ClaI
   DraIII     DrdI     EciI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PciI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SrfI     SwaI     TatI     TstI
  Tth111I     XbaI     XhoI     XmaI     ZraI
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