Restriction map for pF1KE0329
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# NO CUT ENZYMES (  135 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwI        
AocI        ApaI        ApaLI       AscI        Asp718I     
AspEI       AspHI       AspI        AviII       AvrII       
BalI        BamHI       BanII       BbrPI       BbvCI       
BcgI        BciVI       BclI        BcnI        BfrI        
BglII       BlnI        BlpI        BmyI        Bpu1102I    
BsaAI       BsaBI       BsaWI       BseAI       BsiHKAI     
BsiWI       BsiYI       BslI        BsmFI       Bsp1286I    
BspEI       BspHI       BspLU11I    BsrDI       BsrFI       
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstXI       BstYI       BstZ17I     Bsu36I      
CelII       Cfr10I      CpoI        DraIII      DrdI        
DsaI        Eam1105I    Eco81I      EcoNI       EcoO65I     
EcoRV       FauI        FbaI        FseI        FspI        
HincII      HindII      HindIII     HpaI        KpnI        
KspI        MamI        MfeI        MflI        MluI        
MluNI       MroI        MscI        MunI        NaeI        
NciI        NcoI        NdeI        NgoMIV      NotI        
NspI        NspV        PacI        PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
Psp1406I    PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspI        StuI        SwaI        Tsp45I      Tth111I     
Van91I      XcmI        XhoII       XmaCI       XmaI        


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# ONE CUT ENZYMES (   58 enzymes) #
###################################

AcyI           16  GGCGCC               G[GA]^CG[CT]C
AlwNI         494  CAGATACTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI      1162  AGCGCT               AGC^GCT
AseI         1281  ATTAAT               AT^TAAT
AsnI         1281  ATTAAT               AT^TAAT
Asp700I       284  GAAGTGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI          791  CTCGAG               C^[CT]CG[GA]G
BanI           16  GGCGCC               G^G[CT][GA]CC
BbeI           16  GGCGCC               GGCGC^C
BglI           19  GCCATTCCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI          16  GGCGCC               G[GA]^CG[CT]C
BsaI          567  GAGACC               GGTCTC(1/5)
BsiEI          26  CGGCCG               CG[GA][CT]^CG
BsmBI        1424  GAGACG               CGTCTC(1/5)
BsmI          746  GAATGC               GAATGC(1/-1)
BsoBI         791  CTCGAG               C^[CT]CG[GA]G
Bsp1407I     1209  TGTACA               T^GTACA
BspDI         172  ATCGAT               AT^CGAT
BsrBI          51  GAGCGG               CCGCTC(-3/-3)
BsrGI        1209  TGTACA               T^GTACA
BstAPI        520  GCAGGCCATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI          172  ATCGAT               AT^CGAT
DpnII         562  GATC                 ^GATC
DraI         1432  TTTAAA               TTT^AAA
DraII         578  AGGACCT              [GA]G^G[ACGT]CC[CT]
EagI           26  CGGCCG               C^GGCCG
EclXI          26  CGGCCG               C^GGCCG
Eco47III     1162  AGCGCT               AGC^GCT
Eco52I         26  CGGCCG               C^GGCCG
EcoO109I      578  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoT14I       266  CCTTGG               C^C[AT][AT]GG
EcoT22I       334  ATGCAT               ATGCA^T
HapII          25  CCGG                 C^CGG
HgaI          406  GCGTC                GACGC(5/10)
Hin1I          16  GGCGCC               G[GA]^CG[CT]C
HpaII          25  CCGG                 C^CGG
KasI           16  GGCGCC               G^GCGCC
MboI          562  GATC                 ^GATC
MslI          588  CATCCGAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I       1221  CAGCGG               C[AC]G^C[GT]G
MspI           25  CCGG                 C^CGG
NarI           16  GGCGCC               GG^CGCC
NdeII         562  GATC                 ^GATC
NheI          894  GCTAGC               G^CTAGC
NruI           35  TCGCGA               TCG^CGA
NsiI          334  ATGCAT               ATGCA^T
PaeR7I        791  CTCGAG               C^TCGAG
PleI         1464  GACTC                GAGTC(4/5)
PpuMI         578  AGGACCT              [GA]G^G[AT]CC[CT]
PshBI        1281  ATTAAT               AT^TAAT
Sau3AI        562  GATC                 ^GATC
SfoI           16  GGCGCC               GGC^GCC
SspBI        1209  TGTACA               T^GTACA
StyI          266  CCTTGG               C^C[AT][AT]GG
TfiI          928  GAATC                G^A[AT]TC
XbaI         1023  TCTAGA               T^CTAGA
XhoI          791  CTCGAG               C^TCGAG
XmnI          284  GAAGTGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AccI           96  GTCTAC               GT^[AC][GT]AC
              393  GTCTAC              
AccII          36  CGCG                 CG^CG
               57  CGCG                
AfaI         1174  GTAC                 GT^AC
             1210  GTAC                
BpmI          751  CTGGAG               CTGGAG(16/14)
             1275  CTGGAG              
BspMI         570  ACCTGC               ACCTGC(4/8)
               79  GCAGGT              
BssKI          12  CCAGG                ^CC[ACGT]GG
             1100  CCAGG               
BstNI          12  CCAGG                CC^[AT]GG
             1100  CCAGG               
BstUI          36  CGCG                 CG^CG
               57  CGCG                
EaeI           26  CGGCCG               [CT]^GGCC[GA]
               41  CGGCCA              
EcoRII         12  CCAGG                ^CC[AT]GG
             1100  CCAGG               
HaeII          16  GGCGCC               [GA]GCGC^[CT]
             1162  AGCGCT              
HinfI         928  GAATC                G^A[ACGT]TC
             1464  GACTC               
HphI           82  GGTGA                GGTGA(8/7)
             1477  GGTGA               
MaeII         798  ACGT                 A^CGT
             1419  ACGT                
MvaI           12  CCAGG                CC^[AT]GG
             1100  CCAGG               
MvnI           36  CGCG                 CG^CG
               57  CGCG                
NlaIII        526  CATG                 CATG^
             1092  CATG                
PstI          293  CTGCAG               CTGCA^G
              572  CTGCAG              
RsaI         1174  GTAC                 GT^AC
             1210  GTAC                
ScrFI          12  CCAGG                CC^[ACGT]GG
             1100  CCAGG               
SmlI          791  CTCGAG               C^T[CT][GA]AG
             1466  CTCAAG              
TaqI          173  TCGA                 T^CGA
              792  TCGA                
TthHB8I       173  TCGA                 T^CGA
              792  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   42 enzymes) #
#########################################

AciI        AcsI        AluI        ApoI        AvaII       
BbsI        BbvI        BfaI        BpuAI       BsaJI       
BseRI       BsgI        BsmAI       BsrI        BstF5I      
Cac8I       CfoI        Cfr13I      DdeI        EarI        
EcoRI       Fnu4HI      FokI        HaeIII      HhaI        
HinP1I      ItaI        Ksp632I     MaeI        MaeIII      
MboII       MnlI        MseI        MwoI        NlaIV       
Sau96I      SfaNI       SfcI        Tru9I       TseI        
Tsp509I     TspRI       
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