Restriction map for pF1KE0329
 (Linear) (Six-base) MAPSORT of: KIEE0329.seq  Check: 7547  from: 1  to: 1524



KIEE0329.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:13  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      70    1524 
   Size:         70    1454
 
AccI GT'mk_AC

Cuts at:      0      97     394    1524 
   Size:         97     297    1130

  Fragments arranged by size: 

                1130     297      97
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1310    1524 
   Size:       1310     214
 
AfeI AGC'GCT

Cuts at:      0    1164    1524 
   Size:       1164     360
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1061    1524 
   Size:       1061     463
 
AlwNI CAG_nnn'CTG

Cuts at:      0     499    1524 
   Size:        499    1025
 
ApoI r'AATT_y

Cuts at:      0     260     314     962    1108    1448    1506    1524 
   Size:        260      54     648     146     340      58      18

  Fragments arranged by size: 

                 648     340     260     146      58      54      18
 
AseI AT'TA_AT

Cuts at:      0    1282    1524 
   Size:       1282     242
 
AvaI C'yCGr_G

Cuts at:      0     791    1524 
   Size:        791     733
 
BanI G'GyrC_C

Cuts at:      0      16    1524 
   Size:         16    1508
 
BbeI G_GCGC'C

Cuts at:      0      20    1524 
   Size:         20    1504
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      54     967    1197    1524 
   Size:         54     913     230     327

  Fragments arranged by size: 

                 913     327     230      54
 
BfrBI ATG'CAT

Cuts at:      0     336    1524 
   Size:        336    1188
 
BglI GCCn_nnn'nGGC

Cuts at:      0      25    1524 
   Size:         25    1499
 
BmtI G_CTAG'C

Cuts at:      0     898    1524 
   Size:        898     626
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     857    1524 
   Size:        857     667
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     772    1296    1524 
   Size:        772     524     228

  Fragments arranged by size: 

                 772     524     228
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1451    1524 
   Size:       1451      73
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     561    1524 
   Size:        561     963
 
BsaHI Gr'CG_yC

Cuts at:      0      17    1524 
   Size:         17    1507
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     524     696    1010    1524 
   Size:        524     172     314     514

  Fragments arranged by size: 

                 524     514     314     172
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     539     848    1031    1524 
   Size:        539     309     183     493

  Fragments arranged by size: 

                 539     493     309     183
 
BsiEI CG_ry'CG

Cuts at:      0      29    1524 
   Size:         29    1495
 
BsmI GAATG_Cn'

Cuts at:      0     752    1524 
   Size:        752     772
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1418    1524 
   Size:       1418     106
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      70     579    1524 
   Size:         70     509     945

  Fragments arranged by size: 

                 945     509      70
 
BsrBI CCG'CTC

Cuts at:      0      53    1524 
   Size:         53    1471
 
BsrGI T'GTAC_A

Cuts at:      0    1209    1524 
   Size:       1209     315
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     526    1524 
   Size:        526     998
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      18    1524 
   Size:         18    1506
 
ClaI AT'CG_AT

Cuts at:      0     173    1524 
   Size:        173    1351
 
DraI TTT'AAA

Cuts at:      0    1434    1524 
   Size:       1434      90
 
EaeI y'GGCC_r

Cuts at:      0      26      41    1524 
   Size:         26      15    1483

  Fragments arranged by size: 

                1483      26      15
 
EagI C'GGCC_G

Cuts at:      0      26    1524 
   Size:         26    1498
 
EarI CTCTTCn'nnn_

Cuts at:      0     626    1022    1221    1524 
   Size:        626     396     199     303

  Fragments arranged by size: 

                 626     396     303     199
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1298    1524 
   Size:       1298     226
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     772    1296    1310    1524 
   Size:        772     524      14     214

  Fragments arranged by size: 

                 772     524     214      14
 
EcoO109I rG'GnC_Cy

Cuts at:      0     579    1524 
   Size:        579     945
 
EcoRI G'AATT_C

Cuts at:      0     260     314     962    1524 
   Size:        260      54     648     562

  Fragments arranged by size: 

                 648     562     260      54
 
HaeII r_GCGC'y

Cuts at:      0      20    1166    1524 
   Size:         20    1146     358

  Fragments arranged by size: 

                1146     358      20
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      79     111     475     507    1524 
   Size:         79      32     364      32    1017

  Fragments arranged by size: 

                1017     364      79      32      32
 
KasI G'GCGC_C

Cuts at:      0      16    1524 
   Size:         16    1508
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     351     686    1524 
   Size:        351     335     838

  Fragments arranged by size: 

                 838     351     335
 
MslI CAynn'nnrTG

Cuts at:      0     592    1524 
   Size:        592     932
 
MspA1I CmG'CkG

Cuts at:      0    1223    1524 
   Size:       1223     301
 
NarI GG'CG_CC

Cuts at:      0      17    1524 
   Size:         17    1507
 
NheI G'CTAG_C

Cuts at:      0     894    1524 
   Size:        894     630
 
NruI TCG'CGA

Cuts at:      0      37    1524 
   Size:         37    1487
 
NsiI A_TGCA'T

Cuts at:      0     338    1524 
   Size:        338    1186
 
PpuMI rG'GwC_Cy

Cuts at:      0     579    1524 
   Size:        579     945
 
PsiI TTA'TAA

Cuts at:      0     713    1127    1524 
   Size:        713     414     397

  Fragments arranged by size: 

                 713     414     397
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     212     244    1524 
   Size:        212      32    1280

  Fragments arranged by size: 

                1280     212      32
 
PstI C_TGCA'G

Cuts at:      0     297     576    1524 
   Size:        297     279     948

  Fragments arranged by size: 

                 948     297     279
 
SfcI C'TryA_G

Cuts at:      0     196     218     293     572    1524 
   Size:        196      22      75     279     952

  Fragments arranged by size: 

                 952     279     196      75      22
 
SfoI GGC'GCC

Cuts at:      0      18    1524 
   Size:         18    1506
 
SmlI C'TyrA_G

Cuts at:      0     791    1466    1524 
   Size:        791     675      58

  Fragments arranged by size: 

                 791     675      58
 
StyI C'CwwG_G

Cuts at:      0     266    1524 
   Size:        266    1258
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     489    1524 
   Size:        489    1035
 
TatI w'GTAC_w

Cuts at:      0    1209    1524 
   Size:       1209     315
 
XbaI T'CTAG_A

Cuts at:      0    1023    1524 
   Size:       1023     501
 
XhoI C'TCGA_G

Cuts at:      0     791    1524 
   Size:        791     733
 
XmnI GAAnn'nnTTC

Cuts at:      0     288    1524 
   Size:        288    1236
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AfeI     AlfI    AlwNI     ApoI     AseI
     AvaI     BanI     BbeI     BbsI    BfrBI     BglI     BmtI     BplI
     BpmI    BpuEI     BsaI    BsaHI    BseRI     BsgI    BsiEI     BsmI
    BsmBI    BspMI    BsrBI    BsrGI   BstAPI    BtgZI     ClaI     DraI
     EaeI     EagI     EarI     EciI  Eco57MI EcoO109I    EcoRI    HaeII
    Hin4I     KasI     MmeI     MslI   MspA1I     NarI     NheI     NruI
     NsiI    PpuMI     PsiI     PsrI     PstI     SfcI     SfoI     SmlI
     StyI    TaqII     TatI     XbaI     XhoI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI    AflII   AflIII     AgeI     AhdI     AleI
     AloI     ApaI    ApaLI     AscI    AsiSI    AvrII     BaeI    BamHI
    BanII    BbvCI     BcgI    BciVI     BclI    BglII     BlpI Bme1580I
    BmgBI     BmrI   Bpu10I    BsaAI    BsaBI    BsaWI    BsaXI    BseYI
  BsiHKAI    BsiWI Bsp1286I    BspEI    BspHI    BsrDI    BsrFI   BssHII
    BssSI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
     BtsI    CspCI   DraIII     DrdI  EcoICRI    EcoNI    EcoRV     FalI
     FseI     FspI    FspAI   HincII  HindIII     HpaI     KpnI     MfeI
     MluI     MscI     NaeI     NcoI     NdeI   NgoMIV     NotI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PshAI   PspOMI    PspXI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI     TstI  Tth111I     XcmI     XmaI     ZraI
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