Restriction map for
pF1KE0314
(Circular) (Six-base) MAPSORT of: pF1KE0314.seq Check: 2640 from: 1 to: 5261
pF1KE0314 5261 bp
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1603 1603
Size: 5261
AccI GT'mk_AC
Cuts at: 2299 2299
Size: 5261
Acc65I G'GTAC_C
Cuts at: 2277 2277
Size: 5261
AclI AA'CG_TT
Cuts at: 5101 5101
Size: 5261
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 650 1057 2120 3086 3518 4265 4533 650
Size: 407 1063 966 432 747 268 1378
Fragments arranged by size:
1378 1063 966 747 432 407 268
AfeI AGC'GCT
Cuts at: 911 911
Size: 5261
AflII C'TTAA_G
Cuts at: 1144 1144
Size: 5261
AflIII A'CryG_T
Cuts at: 1564 2253 2613 3717 4694 1564
Size: 689 360 1104 977 2131
Fragments arranged by size:
2131 1104 977 689 360
AgeI A'CCGG_T
Cuts at: 3649 3649
Size: 5261
AlwNI CAG_nnn'CTG
Cuts at: 589 970 2566 4133 589
Size: 381 1596 1567 1717
Fragments arranged by size:
1717 1596 1567 381
ApaLI G'TGCA_C
Cuts at: 4031 4031
Size: 5261
ApoI r'AATT_y
Cuts at: 949 1084 1520 1551 2265 4614 4870 949
Size: 135 436 31 714 2349 256 1340
Fragments arranged by size:
2349 1340 714 436 256 135 31
AseI AT'TA_AT
Cuts at: 20 568 20
Size: 548 4713
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5261
AvaI C'yCGr_G
Cuts at: 1118 1790 2281 1118
Size: 672 491 4098
Fragments arranged by size:
4098 672 491
AvrII C'CTAG_G
Cuts at: 664 664
Size: 5261
BamHI G'GATC_C
Cuts at: 930 1736 2286 930
Size: 806 550 3905
Fragments arranged by size:
3905 806 550
BanI G'GyrC_C
Cuts at: 804 1382 2277 2691 2939 2974 804
Size: 578 895 414 248 35 3091
Fragments arranged by size:
3091 895 578 414 248 35
BanII G_rGCy'C
Cuts at: 701 1241 1272 2275 3305 701
Size: 540 31 1003 1030 2657
Fragments arranged by size:
2657 1030 1003 540 31
BbeI G_GCGC'C
Cuts at: 2695 2943 2695
Size: 248 5013
BbsI GAAGACnn'nnnn_
Cuts at: 512 596 2108 512
Size: 84 1512 3665
Fragments arranged by size:
3665 1512 84
BciVI GTATCCnnnnn_n'
Cuts at: 671 3159 3920 5261 671
Size: 2488 761 1341 671
Fragments arranged by size:
2488 1341 761 671
BclI T'GATC_A
Cuts at: 2781 2781
Size: 5261
BfrBI ATG'CAT
Cuts at: 1489 1501 1489
Size: 12 5249
BglI GCCn_nnn'nGGC
Cuts at: 226 226
Size: 5261
BglII A'GATC_T
Cuts at: 529 2776 4813 529
Size: 2247 2037 977
Fragments arranged by size:
2247 2037 977
BlpI GC'TnA_GC
Cuts at: 2373 2373
Size: 5261
Bme1580I G_kGCm'C
Cuts at: 809 1387 1655 2886 2979 4035 809
Size: 578 268 1231 93 1056 2035
Fragments arranged by size:
2035 1231 1056 578 268 93
BmrI ACTGGGnnnn_n'
Cuts at: 186 2473 2554 2889 5033 186
Size: 2287 81 335 2144 414
Fragments arranged by size:
2287 2144 414 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1049 3628 1049
Size: 2579 2682
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 129 136 197 129
Size: 7 61 5193
Fragments arranged by size:
5193 61 7
Bpu10I CC'TnA_GC
Cuts at: 692 1796 692
Size: 1104 4157
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1720 2083 2437 3316 3808 4106 1720
Size: 363 354 879 492 298 2875
Fragments arranged by size:
2875 879 492 363 354 298
BsaI GGTCTCn'nnnn_
Cuts at: 1037 4965 1037
Size: 3928 1333
BsaAI yAC'GTr
Cuts at: 2614 2614
Size: 5261
BsaBI GATnn'nnATC
Cuts at: 6 534 2775 4812 6
Size: 528 2241 2037 455
Fragments arranged by size:
2241 2037 528 455
BsaHI Gr'CG_yC
Cuts at: 2692 2940 3642 5138 2692
Size: 248 702 1496 2815
Fragments arranged by size:
2815 1496 702 248
BsaWI w'CCGG_w
Cuts at: 822 2450 2971 3649 3923 4070 4481 822
Size: 1628 521 678 274 147 411 1602
Fragments arranged by size:
1628 1602 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 170 200 170
Size: 30 5231
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 271 274 400 615 649 880 1286 271
Size: 3 126 215 34 231 406 4246
Fragments arranged by size:
4246 406 231 215 126 34 3
BseYI C'CCAG_C
Cuts at: 280 2686 3444 4021 4517 280
Size: 2406 758 577 496 1024
Fragments arranged by size:
2406 1024 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1631 1631
Size: 5261
BsiEI CG_ry'CG
Cuts at: 104 2803 2849 4057 104
Size: 2699 46 1208 1308
Fragments arranged by size:
2699 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 701 1241 2275 3053 3243 4035 701
Size: 540 1034 778 190 792 1927
Fragments arranged by size:
1927 1034 792 778 540 190
BsmBI CGTCTCn'nnnn_
Cuts at: 2077 2077
Size: 5261
Bsp1286I G_dGCh'C
Cuts at: 701 809 1241 1272 1387 1655 2275 2886 2979
Size: 108 432 31 115 268 620 611 93
Cuts at: 2979 3053 3243 3305 4035 701
Size: 74 190 62 730 1927
Fragments arranged by size:
1927 730 620 611 432 268 190 115
108 93 74 62 31
BspEI T'CCGG_A
Cuts at: 4481 4481
Size: 5261
BspHI T'CATG_A
Cuts at: 4437 4437
Size: 5261
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1603 2311 2525 2827 3208 1603
Size: 708 214 302 381 3656
Fragments arranged by size:
3656 708 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3553 3607 5091 5256 50
Size: 3503 54 1484 165 55
Fragments arranged by size:
3503 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3173 3173
Size: 5261
BsrFI r'CCGG_y
Cuts at: 3259 3440 3649 3259
Size: 181 209 4871
Fragments arranged by size:
4871 209 181
BssSI C'ACGA_G
Cuts at: 3532 3890 3532
Size: 358 4903
BstAPI GCAn_nnn'nTGC
Cuts at: 2638 2638
Size: 5261
BstBI TT'CG_AA
Cuts at: 3622 3622
Size: 5261
BstYI r'GATC_y
Cuts at: 1 529 930 1736 2286 2753 2776 3111 3357
Size: 528 401 806 550 467 23 335 246
Cuts at: 3357 4369 4455 4467 4813 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 806 550 528 467 449 401 346
335 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1252 1252
Size: 5261
BtgI C'CryG_G
Cuts at: 107 1655 107
Size: 1548 3713
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3211 3392 3555 3211
Size: 181 163 4917
Fragments arranged by size:
4917 181 163
BtsI GCAGTG_nn'
Cuts at: 1933 2504 1933
Size: 571 4690
ClaI AT'CG_AT
Cuts at: 1167 1167
Size: 5261
DraI TTT'AAA
Cuts at: 476 1083 1173 1556 2260 476
Size: 607 90 383 704 3477
Fragments arranged by size:
3477 704 607 383 90
DraIII CAC_nnn'GTG
Cuts at: 126 126
Size: 5261
DrdI GACnn_nn'nnGTC
Cuts at: 2967 3825 2967
Size: 858 4403
EaeI y'GGCC_r
Cuts at: 110 2846 3020 3411 3438 4783 110
Size: 2736 174 391 27 1345 588
Fragments arranged by size:
2736 1345 588 391 174 27
EagI C'GGCC_G
Cuts at: 2846 2846
Size: 5261
EarI CTCTTCn'nnn_
Cuts at: 248 401 528 626 920 1229 1718 2225 3284
Size: 153 127 98 294 309 489 507 1059
Cuts at: 3284 3494 248
Size: 210 2015
Fragments arranged by size:
2015 1059 507 489 309 294 210 153
127 98
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3469 3779 3925 5071 3469
Size: 310 146 1146 3659
Fragments arranged by size:
3659 1146 310 146
EcoICRI GAG'CTC
Cuts at: 699 1239 2273 699
Size: 540 1034 3687
Fragments arranged by size:
3687 1034 540
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 129 136 197 650 1057 2120 3086 3518 4265
Size: 7 61 453 407 1063 966 432 747
Cuts at: 4265 4533 129
Size: 268 857
Fragments arranged by size:
1063 966 857 747 453 432 407 268
61 7
EcoNI CCTnn'n_nnAGG
Cuts at: 593 593
Size: 5261
EcoO109I rG'GnC_Cy
Cuts at: 207 219 1404 2400 207
Size: 12 1185 996 3068
Fragments arranged by size:
3068 1185 996 12
EcoRI G'AATT_C
Cuts at: 2265 2265
Size: 5261
EcoRV GAT'ATC
Cuts at: 891 891
Size: 5261
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 529 1075 3280 529
Size: 546 2205 2510
Fragments arranged by size:
2510 2205 546
FspI TGC'GCA
Cuts at: 3042 3042
Size: 5261
HaeII r_GCGC'y
Cuts at: 913 2695 2943 3965 4944 913
Size: 1782 248 1022 979 1230
Fragments arranged by size:
1782 1230 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 170 202 3103 3133 3135 3165 170
Size: 32 2901 30 2 30 2266
Fragments arranged by size:
2901 2266 32 30 30 2
HincII GTy'rAC
Cuts at: 2300 2300
Size: 5261
HindIII A'AGCT_T
Cuts at: 2112 2112
Size: 5261
KasI G'GCGC_C
Cuts at: 2691 2939 2691
Size: 248 5013
KpnI G_GTAC'C
Cuts at: 2281 2281
Size: 5261
MluI A'CGCG_T
Cuts at: 4694 4694
Size: 5261
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1697 3932 4116 1697
Size: 2235 184 2842
Fragments arranged by size:
2842 2235 184
MscI TGG'CCA
Cuts at: 112 3022 112
Size: 2910 2351
MslI CAynn'nnrTG
Cuts at: 380 3250 3377 380
Size: 2870 127 2264
Fragments arranged by size:
2870 2264 127
MspA1I CmG'CkG
Cuts at: 232 280 772 1131 2372 2686 3046 4059 4304
Size: 48 492 359 1241 314 360 1013 245
Cuts at: 4304 232
Size: 1189
Fragments arranged by size:
1241 1189 1013 492 360 359 314 245
48
NaeI GCC'GGC
Cuts at: 3442 3442
Size: 5261
NarI GG'CG_CC
Cuts at: 2692 2940 2692
Size: 248 5013
NcoI C'CATG_G
Cuts at: 107 107
Size: 5261
NgoMIV G'CCGG_C
Cuts at: 3440 3440
Size: 5261
NsiI A_TGCA'T
Cuts at: 1491 1503 1491
Size: 12 5249
NspI r_CATG'y
Cuts at: 331 1501 2314 2814 3721 331
Size: 1170 813 500 907 1871
Fragments arranged by size:
1871 1170 907 813 500
PciI A'CATG_T
Cuts at: 3717 3717
Size: 5261
PflMI CCAn_nnn'nTGG
Cuts at: 126 126
Size: 5261
PmeI GTTT'AAAC
Cuts at: 2260 2260
Size: 5261
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4400 4432 4400
Size: 32 5229
PpuMI rG'GwC_Cy
Cuts at: 207 1404 207
Size: 1197 4064
PstI C_TGCA'G
Cuts at: 2308 2993 2308
Size: 685 4576
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5261
PvuII CAG'CTG
Cuts at: 772 1131 2686 3046 772
Size: 359 1555 360 2987
Fragments arranged by size:
2987 1555 360 359
RsrII CG'GwC_CG
Cuts at: 3456 3456
Size: 5261
SacI G_AGCT'C
Cuts at: 701 1241 2275 701
Size: 540 1034 3687
Fragments arranged by size:
3687 1034 540
SalI G'TCGA_C
Cuts at: 2298 2298
Size: 5261
SapI GCTCTTCn'nnn_
Cuts at: 1229 3284 3494 1229
Size: 2055 210 2996
Fragments arranged by size:
2996 2055 210
SbfI CC_TGCA'GG
Cuts at: 2308 2308
Size: 5261
ScaI AGT'ACT
Cuts at: 2060 2068 4778 2060
Size: 8 2710 2543
Fragments arranged by size:
2710 2543 8
SfcI C'TryA_G
Cuts at: 33 2304 2989 3982 4173 33
Size: 2271 685 993 191 1121
Fragments arranged by size:
2271 1121 993 685 191
SfoI GGC'GCC
Cuts at: 2693 2941 2693
Size: 248 5013
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5261
SmaI CCC'GGG
Cuts at: 1120 1792 2283 1120
Size: 672 491 4098
Fragments arranged by size:
4098 672 491
SmlI C'TyrA_G
Cuts at: 1144 1699 2062 2416 3331 3823 4085 1144
Size: 555 363 354 915 492 262 2320
Fragments arranged by size:
2320 915 555 492 363 354 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5261
SphI G_CATG'C
Cuts at: 2314 2814 2314
Size: 500 4761
StyI C'CwwG_G
Cuts at: 107 664 1585 2395 2749 107
Size: 557 921 810 354 2619
Fragments arranged by size:
2619 921 810 557 354
SwaI ATTT'AAAT
Cuts at: 1083 1083
Size: 5261
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 792 2844 2977 3436 3645 792
Size: 2052 133 459 209 2408
Fragments arranged by size:
2408 2052 459 209 133
TatI w'GTAC_w
Cuts at: 2058 2066 4776 2058
Size: 8 2710 2543
Fragments arranged by size:
2710 2543 8
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4778 4810 4778
Size: 32 5229
Tth111I GACn'n_nGTC
Cuts at: 3058 3058
Size: 5261
XbaI T'CTAG_A
Cuts at: 2292 2292
Size: 5261
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2756 2756
Size: 5261
XmaI C'CCGG_G
Cuts at: 1118 1790 2281 1118
Size: 672 491 4098
Fragments arranged by size:
4098 672 491
XmnI GAAnn'nnTTC
Cuts at: 949 949
Size: 5261
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PciI PflMI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI SwaI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AhdI AleI AlfI AloI ApaI AscI BaeI
BbvCI BcgI BmgBI BmtI BsiWI BsmI BsrGI BssHII
BstEII BstXI BstZ17I CspCI FseI FspAI HpaI MfeI
NdeI NheI NotI NruI PacI PasI PfoI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI XhoI ZraI