Restriction map for pF1KE1864
##################################
# NO CUT ENZYMES (  127 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AflII       AflIII      AgeI        Alw44I      
Aor51HI     ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspI        AvaI        AviII       
AvrII       BamHI       BbrPI       BcgI        BciVI       
BclI        BfrI        BglI        BglII       BlnI        
BsaAI       BsaBI       BsaWI       BseAI       BsiEI       
BsiWI       BsmBI       BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstXI       
BstZ17I     Cfr10I      ClaI        CpoI        DraI        
DrdI        EagI        EclXI       Eco47III    Eco52I      
EcoNI       EcoO65I     EcoRI       FbaI        FseI        
FspI        HincII      HindII      HindIII     HpaI        
KpnI        KspI        MamI        MfeI        MluI        
MroI        MseI        MunI        MvnI        NaeI        
NdeI        NgoMIV      NheI        NotI        NruI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacII       
SalI        ScaI        SexAI       SfiI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SspBI       SspI        SwaI        TaqI        Tru9I       
Tth111I     TthHB8I     XcmI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AcsI          498  GAATTT               [GA]^AATT[CT]
AfaI          145  GTAC                 GT^AC
AhdI          711  GACCTGGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI            9  GGGCCC               GGGCC^C
ApoI          498  GAATTT               [GA]^AATT[CT]
AspEI         711  GACCTGGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI         1150  GGCGCC               G^G[CT][GA]CC
BbeI         1150  GGCGCC               GGCGC^C
BbvCI         139  GCTGAGG              CCTCAGC(-5/-2)
BfaI          583  CTAG                 C^TAG
BlpI           67  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I       67  GCTGAGC              GC^T[ACGT]AGC
BsaI          253  GAGACC               GGTCTC(1/5)
BsgI          121  GTGCAG               GTGCAG(16/14)
BsmI          330  GCATTC               GAATGC(1/-1)
BssSI        1033  CTCGTG               CACGAG(-5/-1)
CelII          67  GCTGAGC              GC^T[ACGT]AGC
DraIII        877  CACCCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          620  CCATGG               C^C[GA][CT]GG
Eam1105I      711  GACCTGGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV         578  GATATC               GAT^ATC
EcoT22I       547  ATGCAT               ATGCA^T
HaeII        1150  GGCGCC               [GA]GCGC^[CT]
HgaI          717  GCGTC                GACGC(5/10)
KasI         1150  GGCGCC               G^GCGCC
MaeI          583  CTAG                 C^TAG
NarI         1150  GGCGCC               GG^CGCC
NcoI          620  CCATGG               C^CATGG
NsiI          547  ATGCAT               ATGCA^T
PflMI         426  CCAAAACGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          912  GAGTC                GAGTC(4/5)
PvuII        1013  CAGCTG               CAG^CTG
RsaI          145  GTAC                 GT^AC
SapI          414  GCTCTTC              GCTCTTC(1/4)
SfoI         1150  GGCGCC               GGC^GCC
SphI          549  GCATGC               GCATG^C
StuI          644  AGGCCT               AGG^CCT
Tsp509I       499  AATT                 ^AATT
Van91I        426  CCAAAACGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI          582  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AciI          636  CCGC                 CCGC(-3/-1)
              860  GCGG                
AcyI          716  GGCGTC               G[GA]^CG[CT]C
             1150  GGCGCC              
AlwNI        1045  CAGGTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1108  CAGCCTCTG           
AocI          285  CCTCAGG              CC^T[ACGT]AGG
              600  CCTGAGG             
BalI          482  TGGCCA               TGG^CCA
              803  TGGCCA              
BpmI          337  CTCCAG               CTGGAG(16/14)
              465  CTCCAG              
BsaHI         716  GGCGTC               G[GA]^CG[CT]C
             1150  GGCGCC              
BseRI         603  GAGGAG               GAGGAG(10/8)
              826  GAGGAG              
BsmAI         253  GAGAC                GTCTC(1/5)
              436  GAGAC               
BspMI         841  ACCTGC               ACCTGC(4/8)
             1044  GCAGGT              
BstYI         115  GGATCT               [GA]^GATC[CT]
              359  AGATCC              
Bsu36I        285  CCTCAGG              CC^T[ACGT]AGG
              600  CCTGAGG             
CfoI          171  GCGC                 GCG^C
             1151  GCGC                
DraII         281  AGGACCT              [GA]G^G[ACGT]CC[CT]
             1046  AGGTCCT             
EaeI          482  TGGCCA               [CT]^GGCC[GA]
              803  TGGCCA              
EarI          415  CTCTTC               CTCTTC(1/4)
              608  GAAGAG              
Eco81I        285  CCTCAGG              CC^T[ACGT]AGG
              600  CCTGAGG             
EcoO109I      281  AGGACCT              [GA]G^G[ACGT]CC[CT]
             1046  AGGTCCT             
FauI          635  CCCGC                CCCGC(4/6)
              860  GCGGG               
HhaI          171  GCGC                 GCG^C
             1151  GCGC                
Hin1I         716  GGCGTC               G[GA]^CG[CT]C
             1150  GGCGCC              
HinP1I        171  GCGC                 G^CGC
             1151  GCGC                
Ksp632I       415  CTCTTC               CTCTTC(1/4)
              608  GAAGAG              
MaeII         431  ACGT                 A^CGT
              491  ACGT                
MflI          115  GGATCT               [GA]^GATC[CT]
              359  AGATCC              
MluNI         482  TGGCCA               TGG^CCA
              803  TGGCCA              
MscI          482  TGGCCA               TGG^CCA
              803  TGGCCA              
MspA1I        858  CAGCGG               C[AC]G^C[GT]G
             1013  CAGCTG              
NspI          549  GCATGC               [GA]CATG^[CT]
              670  ACATGC              
PpuMI         281  AGGACCT              [GA]G^G[AT]CC[CT]
             1046  AGGTCCT             
SbfI          561  CCTGCAGG             CCTGCA^GG
             1041  CCTGCAGG            
Sse8387I      561  CCTGCAGG             CCTGCA^GG
             1041  CCTGCAGG            
TfiI          586  GAATC                G^A[AT]TC
              864  GAATC               
XhoII         115  GGATCT               [GA]^GATC[CT]
              359  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   57 enzymes) #
#########################################

AluI        AlwI        AspHI       AvaII       BanII       
BbsI        BbvI        BcnI        BmyI        BpuAI       
BsaJI       BsiHKAI     BsiYI       BslI        BsmFI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
Cac8I       Cfr13I      DdeI        DpnII       EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      HapII       
HinfI       HpaII       HphI        ItaI        MaeIII      
MboI        MboII       MnlI        MslI        MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       PstI        SacI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        StyI        TseI        
Tsp45I      TspRI       
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