Restriction map for pF1KE0288
##################################
# NO CUT ENZYMES (  131 enzymes) #
##################################

AatII       AccII       AccIII      AclI        AflII       
AflIII      AgeI        AhdI        Alw44I      Aor51HI     
ApaLI       AscI        AseI        AsnI        Asp700I     
AspEI       AspI        AvaI        AvaII       AviII       
AvrII       BalI        BbrPI       BbsI        BbvCI       
BcgI        BclI        BfrI        BglII       BlnI        
BpuAI       BsaAI       BsaBI       BsaI        BsaWI       
BseAI       BsgI        BsiEI       BsiWI       BsmBI       
BsoBI       BspDI       BspEI       BspLU11I    BsrBI       
BsrDI       BsrFI       BssHII      BssSI       Bst1107I    
BstBI       BstUI       BstZ17I     Cfr10I      ClaI        
CpoI        DraI        DraII       EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      EcoO109I    
EcoRV       EcoT22I     FbaI        FseI        FspI        
HgaI        HindIII     HpaI        Ksp632I     KspI        
MamI        MfeI        MluI        MluNI       MroI        
MscI        MunI        MvnI        NaeI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspI        SwaI        TaqI        Tth111I     
TthHB8I     XbaI        XhoI        XmaCI       XmaI        
XmnI        


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# ONE CUT ENZYMES (   63 enzymes) #
###################################

Acc65I        368  GGTACC               G^GTACC
AccI          604  GTAGAC               GT^[AC][GT]AC
AcsI           47  GAATTC               [GA]^AATT[CT]
AcyI          704  GGCGCC               G[GA]^CG[CT]C
AlwNI         385  CAGCCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          780  CCTGAGG              CC^T[ACGT]AGG
ApaI          511  GGGCCC               GGGCC^C
ApoI           47  GAATTC               [GA]^AATT[CT]
Asp718I       368  GGTACC               G^GTACC
AspHI         403  GTGCTC               G[AT]GC[AT]^C
BamHI         457  GGATCC               G^GATCC
BbeI          704  GGCGCC               GGCGC^C
BciVI          54  GTATCC               GTATCC(6/5)
BcnI          708  CCGGG                CC^[GC]GG
BfaI          823  CTAG                 C^TAG
BglI          445  GCCTGGATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI         704  GGCGCC               G[GA]^CG[CT]C
BsiHKAI       403  GTGCTC               G[AT]GC[AT]^C
BsmAI         558  GAGAC                GTCTC(1/5)
BsmI          905  GAATGC               GAATGC(1/-1)
Bsp1407I      179  TGTACA               T^GTACA
BspMI         125  ACCTGC               ACCTGC(4/8)
BsrGI         179  TGTACA               T^GTACA
BstAPI        329  GCACCCAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        744  GGTCACC              G^GT[ACGT]ACC
BstPI         744  GGTCACC              G^GT[ACGT]ACC
BstXI         515  CCAATCAGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         457  GGATCC               [GA]^GATC[CT]
Bsu36I        780  CCTGAGG              CC^T[ACGT]AGG
DraIII        330  CACCCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          574  GACACAACAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI          833  CGGCCA               [CT]^GGCC[GA]
Eco81I        780  CCTGAGG              CC^T[ACGT]AGG
EcoNI         359  CCTATGACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       744  GGTCACC              G^GT[ACGT]ACC
EcoRI          47  GAATTC               G^AATTC
FauI          170  CCCGC                CCCGC(4/6)
HapII         708  CCGG                 C^CGG
Hin1I         704  GGCGCC               G[GA]^CG[CT]C
HpaII         708  CCGG                 C^CGG
KasI          704  GGCGCC               G^GCGCC
KpnI          368  GGTACC               GGTAC^C
MaeI          823  CTAG                 C^TAG
MaeII         598  ACGT                 A^CGT
MboII         533  TCTTC                GAAGA(8/7)
MflI          457  GGATCC               [GA]^GATC[CT]
MseI          136  TTAA                 T^TAA
MspI          708  CCGG                 C^CGG
NarI          704  GGCGCC               GG^CGCC
NciI          708  CCGGG                CC^[GC]GG
NcoI          465  CCATGG               C^CATGG
PflMI         280  CCATTTGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          111  GACTC                GAGTC(4/5)
PstI          548  CTGCAG               CTGCA^G
SfaNI         218  GCATC                GCATC(5/9)
SfcI          548  CTGCAG               C^T[GA][CT]AG
SfoI          704  GGCGCC               GGC^GCC
SspBI         179  TGTACA               T^GTACA
StuI          784  AGGCCT               AGG^CCT
Tru9I         136  TTAA                 T^TAA
Tsp509I        48  AATT                 ^AATT
Van91I        280  CCATTTGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         457  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

BanI          368  GGTACC               G^G[CT][GA]CC
              704  GGCGCC              
BlpI          415  GCTAAGC              GC^T[ACGT]AGC
              420  GCTGAGC             
BpmI          475  CTCCAG               CTGGAG(16/14)
              440  CTGGAG              
Bpu1102I      415  GCTAAGC              GC^T[ACGT]AGC
              420  GCTGAGC             
BseRI         222  CTCCTC               GAGGAG(10/8)
              646  CTCCTC              
BsmFI         235  GTCCC                GGGAC(10/14)
              826  GTCCC               
BspHI         245  TCATGA               T^CATGA
              407  TCATGA              
CelII         415  GCTAAGC              GC^T[ACGT]AGC
              420  GCTGAGC             
EcoT14I       157  CCAAGG               C^C[AT][AT]GG
              465  CCATGG              
HaeII         424  AGCGCC               [GA]GCGC^[CT]
              704  GGCGCC              
HincII         24  GTTGAC               GT[CT]^[GA]AC
              583  GTCAAC              
HindII         24  GTTGAC               GT[CT]^[GA]AC
              583  GTCAAC              
RcaI          245  TCATGA               T^CATGA
              407  TCATGA              
StyI          157  CCAAGG               C^C[AT][AT]GG
              465  CCATGG              
TfiI            8  GAATC                G^A[AT]TC
              683  GAATC               
XcmI          687  CCACACAGCTGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              794  CCAACAGCCCCCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   48 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        BanII       
BbvI        BmyI        BsaJI       BsiYI       BslI        
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DsaI        EcoRII      Fnu4HI      FokI        HaeIII      
HhaI        HinP1I      HinfI       HphI        ItaI        
MaeIII      MboI        MnlI        MslI        MspA1I      
MvaI        MwoI        NdeII       NlaIII      NlaIV       
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
TseI        Tsp45I      TspRI       
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