(Circular) (Six-base) MAPSORT of: pF1KE0275.seq Check: 7395 from: 1 to: 3635 pF1KE0275 3635 bp With 182 enzymes: SgfI * April 24, 2009 13:12 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 339 339 Size: 3635 AccI GT'mk_AC Cuts at: 673 673 Size: 3635 Acc65I G'GTAC_C Cuts at: 651 651 Size: 3635 AclI AA'CG_TT Cuts at: 3475 3475 Size: 3635 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1460 1892 2639 2907 1460 Size: 432 747 268 2188 Fragments arranged by size: 2188 747 432 268 AfeI AGC'GCT Cuts at: 407 407 Size: 3635 AflIII A'CryG_T Cuts at: 987 2091 3068 987 Size: 1104 977 1554 Fragments arranged by size: 1554 1104 977 AgeI A'CCGG_T Cuts at: 2023 2023 Size: 3635 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 565 565 Size: 3635 AlwNI CAG_nnn'CTG Cuts at: 940 2507 940 Size: 1567 2068 ApaLI G'TGCA_C Cuts at: 2405 2405 Size: 3635 ApoI r'AATT_y Cuts at: 639 2988 3244 639 Size: 2349 256 1030 Fragments arranged by size: 2349 1030 256 AseI AT'TA_AT Cuts at: 20 20 Size: 3635 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3635 AvaI C'yCGr_G Cuts at: 655 655 Size: 3635 BamHI G'GATC_C Cuts at: 660 660 Size: 3635 BanI G'GyrC_C Cuts at: 651 1065 1313 1348 651 Size: 414 248 35 2938 Fragments arranged by size: 2938 414 248 35 BanII G_rGCy'C Cuts at: 161 524 649 1679 161 Size: 363 125 1030 2117 Fragments arranged by size: 2117 1030 363 125 BbeI G_GCGC'C Cuts at: 1069 1317 1069 Size: 248 3387 BbsI GAAGACnn'nnnn_ Cuts at: 133 133 Size: 3635 BciVI GTATCCnnnnn_n' Cuts at: 1533 2294 3635 1533 Size: 761 1341 1533 Fragments arranged by size: 1533 1341 761 BclI T'GATC_A Cuts at: 1155 1155 Size: 3635 BglI GCCn_nnn'nGGC Cuts at: 503 571 503 Size: 68 3567 BglII A'GATC_T Cuts at: 1150 3187 1150 Size: 2037 1598 BlpI GC'TnA_GC Cuts at: 747 747 Size: 3635 Bme1580I G_kGCm'C Cuts at: 1260 1353 2409 1260 Size: 93 1056 2486 Fragments arranged by size: 2486 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 847 928 1263 3407 847 Size: 81 335 2144 1075 Fragments arranged by size: 2144 1075 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 321 2002 321 Size: 1681 1954 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 256 493 256 Size: 237 3398 Bpu10I CC'TnA_GC Cuts at: 402 402 Size: 3635 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 811 1690 2182 2480 811 Size: 879 492 298 1966 Fragments arranged by size: 1966 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3339 3339 Size: 3635 BsaAI yAC'GTr Cuts at: 988 988 Size: 3635 BsaBI GATnn'nnATC Cuts at: 6 1149 3186 6 Size: 1143 2037 455 Fragments arranged by size: 2037 1143 455 BsaHI Gr'CG_yC Cuts at: 1066 1314 2016 3512 1066 Size: 248 702 1496 1189 Fragments arranged by size: 1496 1189 702 248 BsaWI w'CCGG_w Cuts at: 824 1345 2023 2297 2444 2855 824 Size: 521 678 274 147 411 1604 Fragments arranged by size: 1604 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 448 478 448 Size: 30 3605 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 274 295 472 514 274 Size: 21 177 42 3395 Fragments arranged by size: 3395 177 42 21 BseYI C'CCAG_C Cuts at: 344 411 561 619 1060 1818 2395 2891 344 Size: 67 150 58 441 758 577 496 1088 Fragments arranged by size: 1088 758 577 496 441 150 67 58 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 535 535 Size: 3635 BsiEI CG_ry'CG Cuts at: 104 490 1177 1223 2431 104 Size: 386 687 46 1208 1308 Fragments arranged by size: 1308 1208 687 386 46 BsiHKAI G_wGCw'C Cuts at: 161 524 649 1427 1617 2409 161 Size: 363 125 778 190 792 1387 Fragments arranged by size: 1387 792 778 363 190 125 Bsp1286I G_dGCh'C Cuts at: 161 524 649 1260 1353 1427 1617 1679 2409 Size: 363 125 611 93 74 190 62 730 Cuts at: 2409 161 Size: 1387 Fragments arranged by size: 1387 730 611 363 190 125 93 74 62 BspEI T'CCGG_A Cuts at: 2855 2855 Size: 3635 BspHI T'CATG_A Cuts at: 2811 2811 Size: 3635 BspMI ACCTGCnnnn'nnnn_ Cuts at: 339 685 899 1201 1582 339 Size: 346 214 302 381 2392 Fragments arranged by size: 2392 381 346 302 214 BsrBI CCG'CTC Cuts at: 50 300 1927 1981 3465 3630 50 Size: 250 1627 54 1484 165 55 Fragments arranged by size: 1627 1484 250 165 55 54 BsrDI GCAATG_nn' Cuts at: 1547 1547 Size: 3635 BsrFI r'CCGG_y Cuts at: 1633 1814 2023 1633 Size: 181 209 3245 Fragments arranged by size: 3245 209 181 BssHII G'CGCG_C Cuts at: 366 366 Size: 3635 BssSI C'ACGA_G Cuts at: 1906 2264 1906 Size: 358 3277 BstAPI GCAn_nnn'nTGC Cuts at: 539 1012 539 Size: 473 3162 BstBI TT'CG_AA Cuts at: 1996 1996 Size: 3635 BstYI r'GATC_y Cuts at: 1 476 597 660 1127 1150 1485 1731 2743 Size: 475 121 63 467 23 335 246 1012 Cuts at: 2743 2829 2841 3187 1 Size: 86 12 346 449 Fragments arranged by size: 1012 475 467 449 346 335 246 121 86 63 23 12 BtgI C'CryG_G Cuts at: 107 268 498 107 Size: 161 230 3244 Fragments arranged by size: 3244 230 161 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1585 1766 1929 1585 Size: 181 163 3291 Fragments arranged by size: 3291 181 163 BtsI GCAGTG_nn' Cuts at: 576 878 576 Size: 302 3333 DraI TTT'AAA Cuts at: 634 634 Size: 3635 DrdI GACnn_nn'nnGTC Cuts at: 1341 2199 1341 Size: 858 2777 EaeI y'GGCC_r Cuts at: 1220 1394 1785 1812 3157 1220 Size: 174 391 27 1345 1698 Fragments arranged by size: 1698 1345 391 174 27 EagI C'GGCC_G Cuts at: 1220 1220 Size: 3635 EarI CTCTTCn'nnn_ Cuts at: 149 532 1658 1868 149 Size: 383 1126 210 1916 Fragments arranged by size: 1916 1126 383 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1843 2153 2299 3445 1843 Size: 310 146 1146 2033 Fragments arranged by size: 2033 1146 310 146 EcoICRI GAG'CTC Cuts at: 159 522 647 159 Size: 363 125 3147 Fragments arranged by size: 3147 363 125 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 256 493 1460 1892 2639 2907 256 Size: 237 967 432 747 268 984 Fragments arranged by size: 984 967 747 432 268 237 EcoNI CCTnn'n_nnAGG Cuts at: 454 454 Size: 3635 EcoO109I rG'GnC_Cy Cuts at: 774 774 Size: 3635 EcoRI G'AATT_C Cuts at: 639 639 Size: 3635 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 150 1654 150 Size: 1504 2131 FspI TGC'GCA Cuts at: 1416 1416 Size: 3635 HaeII r_GCGC'y Cuts at: 306 358 409 1069 1317 2339 3318 306 Size: 52 51 660 248 1022 979 623 Fragments arranged by size: 1022 979 660 623 248 52 51 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1477 1507 1509 1539 1477 Size: 30 2 30 3573 Fragments arranged by size: 3573 30 30 2 HincII GTy'rAC Cuts at: 674 674 Size: 3635 KasI G'GCGC_C Cuts at: 1065 1313 1065 Size: 248 3387 KpnI G_GTAC'C Cuts at: 655 655 Size: 3635 MluI A'CGCG_T Cuts at: 3068 3068 Size: 3635 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 182 2306 2490 182 Size: 2124 184 1327 Fragments arranged by size: 2124 1327 184 MscI TGG'CCA Cuts at: 1396 1396 Size: 3635 MslI CAynn'nnrTG Cuts at: 1624 1751 1624 Size: 127 3508 MspA1I CmG'CkG Cuts at: 234 270 324 377 500 619 746 1060 1420 Size: 36 54 53 123 119 127 314 360 Cuts at: 1420 2433 2678 234 Size: 1013 245 1191 Fragments arranged by size: 1191 1013 360 314 245 127 123 119 54 53 36 NaeI GCC'GGC Cuts at: 1816 1816 Size: 3635 NarI GG'CG_CC Cuts at: 1066 1314 1066 Size: 248 3387 NcoI C'CATG_G Cuts at: 107 107 Size: 3635 NgoMIV G'CCGG_C Cuts at: 1814 1814 Size: 3635 NspI r_CATG'y Cuts at: 688 1188 2095 688 Size: 500 907 2228 Fragments arranged by size: 2228 907 500 PciI A'CATG_T Cuts at: 2091 2091 Size: 3635 PflMI CCAn_nnn'nTGG Cuts at: 351 351 Size: 3635 PfoI T'CCnGG_A Cuts at: 452 452 Size: 3635 PmeI GTTT'AAAC Cuts at: 634 634 Size: 3635 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2774 2806 2774 Size: 32 3603 PstI C_TGCA'G Cuts at: 682 1367 682 Size: 685 2950 PvuI CG_AT'CG Cuts at: 104 104 Size: 3635 PvuII CAG'CTG Cuts at: 1060 1420 1060 Size: 360 3275 RsrII CG'GwC_CG Cuts at: 1830 1830 Size: 3635 SacI G_AGCT'C Cuts at: 161 524 649 161 Size: 363 125 3147 Fragments arranged by size: 3147 363 125 SacII CC_GC'GG Cuts at: 271 501 271 Size: 230 3405 SalI G'TCGA_C Cuts at: 672 672 Size: 3635 SapI GCTCTTCn'nnn_ Cuts at: 149 1658 1868 149 Size: 1509 210 1916 Fragments arranged by size: 1916 1509 210 SbfI CC_TGCA'GG Cuts at: 682 682 Size: 3635 ScaI AGT'ACT Cuts at: 3152 3152 Size: 3635 SfcI C'TryA_G Cuts at: 33 678 1363 2356 2547 33 Size: 645 685 993 191 1121 Fragments arranged by size: 1121 993 685 645 191 SfoI GGC'GCC Cuts at: 1067 1315 1067 Size: 248 3387 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3635 SmaI CCC'GGG Cuts at: 657 657 Size: 3635 SmlI C'TyrA_G Cuts at: 790 1705 2197 2459 790 Size: 915 492 262 1966 Fragments arranged by size: 1966 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3635 SphI G_CATG'C Cuts at: 688 1188 688 Size: 500 3135 StyI C'CwwG_G Cuts at: 107 769 1123 107 Size: 662 354 2619 Fragments arranged by size: 2619 662 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 358 575 1218 1351 1810 2019 358 Size: 217 643 133 459 209 1974 Fragments arranged by size: 1974 643 459 217 209 133 TatI w'GTAC_w Cuts at: 3150 3150 Size: 3635 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 448 480 3152 3184 448 Size: 32 2672 32 899 Fragments arranged by size: 2672 899 32 32 Tth111I GACn'n_nGTC Cuts at: 1432 1432 Size: 3635 XbaI T'CTAG_A Cuts at: 666 666 Size: 3635 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1130 1130 Size: 3635 XmaI C'CCGG_G Cuts at: 655 655 Size: 3635 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI PpiI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AatII AflII AhdI AleI AloI ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI BsiWI BsmI BsmBI BsrGI BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI