Restriction map for pF1KE0275
 (Linear) (Six-base) MAPSORT of: KIEE0275.seq  Check: 5211  from: 1  to: 522



KIEE0275.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:12  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     231     522 
   Size:        231     291
 
AfeI AGC'GCT

Cuts at:      0     299     522 
   Size:        299     223
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     457     522 
   Size:        457      65
 
BanII G_rGCy'C

Cuts at:      0      53     416     522 
   Size:         53     363     106

  Fragments arranged by size: 

                 363     106      53
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      25     522 
   Size:         25     497
 
BglI GCCn_nnn'nGGC

Cuts at:      0     395     463     522 
   Size:        395      68      59

  Fragments arranged by size: 

                 395      68      59
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     213     522 
   Size:        213     309
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     148     385     522 
   Size:        148     237     137

  Fragments arranged by size: 

                 237     148     137
 
Bpu10I CC'TnA_GC

Cuts at:      0     294     522 
   Size:        294     228
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     340     370     522 
   Size:        340      30     152

  Fragments arranged by size: 

                 340     152      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     166     187     364     406     522 
   Size:        166      21     177      42     116

  Fragments arranged by size: 

                 177     166     116      42      21
 
BseYI C'CCAG_C

Cuts at:      0     236     303     453     511     522 
   Size:        236      67     150      58      11

  Fragments arranged by size: 

                 236     150      67      58      11
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     427     522 
   Size:        427      95
 
BsiEI CG_ry'CG

Cuts at:      0     382     522 
   Size:        382     140
 
BsiHKAI G_wGCw'C

Cuts at:      0      53     416     522 
   Size:         53     363     106

  Fragments arranged by size: 

                 363     106      53
 
Bsp1286I G_dGCh'C

Cuts at:      0      53     416     522 
   Size:         53     363     106

  Fragments arranged by size: 

                 363     106      53
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     231     522 
   Size:        231     291
 
BsrBI CCG'CTC

Cuts at:      0     192     522 
   Size:        192     330
 
BssHII G'CGCG_C

Cuts at:      0     258     522 
   Size:        258     264
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     431     522 
   Size:        431      91
 
BstYI r'GATC_y

Cuts at:      0     368     489     522 
   Size:        368     121      33

  Fragments arranged by size: 

                 368     121      33
 
BtgI C'CryG_G

Cuts at:      0     160     390     522 
   Size:        160     230     132

  Fragments arranged by size: 

                 230     160     132
 
BtsI GCAGTG_nn'

Cuts at:      0     468     522 
   Size:        468      54
 
EarI CTCTTCn'nnn_

Cuts at:      0      41     424     522 
   Size:         41     383      98

  Fragments arranged by size: 

                 383      98      41
 
EcoICRI GAG'CTC

Cuts at:      0      51     414     522 
   Size:         51     363     108

  Fragments arranged by size: 

                 363     108      51
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     148     385     522 
   Size:        148     237     137

  Fragments arranged by size: 

                 237     148     137
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     346     522 
   Size:        346     176
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      42     522 
   Size:         42     480
 
HaeII r_GCGC'y

Cuts at:      0     198     250     301     522 
   Size:        198      52      51     221

  Fragments arranged by size: 

                 221     198      52      51
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      74     522 
   Size:         74     448
 
MspA1I CmG'CkG

Cuts at:      0     126     162     216     269     392     511     522 
   Size:        126      36      54      53     123     119      11

  Fragments arranged by size: 

                 126     123     119      54      53      36      11
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     243     522 
   Size:        243     279
 
PfoI T'CCnGG_A

Cuts at:      0     344     522 
   Size:        344     178
 
SacI G_AGCT'C

Cuts at:      0      53     416     522 
   Size:         53     363     106

  Fragments arranged by size: 

                 363     106      53
 
SacII CC_GC'GG

Cuts at:      0     163     393     522 
   Size:        163     230     129

  Fragments arranged by size: 

                 230     163     129
 
SapI GCTCTTCn'nnn_

Cuts at:      0      41     522 
   Size:         41     481
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     250     467     522 
   Size:        250     217      55

  Fragments arranged by size: 

                 250     217      55
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     340     372     522 
   Size:        340      32     150

  Fragments arranged by size: 

                 340     150      32
 

 Enzymes that do cut:

     AarI     AfeI     AlfI    BanII     BbsI     BglI     BplI     BpmI
   Bpu10I    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I
    BspMI    BsrBI   BssHII   BstAPI    BstYI     BtgI     BtsI     EarI
  EcoICRI  Eco57MI    EcoNI     FalI    HaeII     MmeI   MspA1I    PflMI
     PfoI     SacI    SacII     SapI    TaqII     TstI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrDI    BsrFI
    BsrGI    BssSI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI
 EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI     MslI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SalI    SanDI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI     TatI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
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