################################## # NO CUT ENZYMES ( 143 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BglII BlpI Bpu1102I BsaAI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrGI BssHII Bst1107I BstEII BstPI BstUI BstZ17I Bsu36I CelII CfoI ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII HpaI KasI KpnI KspI MaeII MfeI MluI MroI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XbaI XhoI ################################### # ONE CUT ENZYMES ( 28 enzymes) # ################################### ApaI 1176 GGGCCC GGGCC^C Asp700I 578 GAAGCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvrII 1168 CCTAGG C^CTAGG BamHI 1102 GGATCC G^GATCC BanII 1176 GGGCCC G[GA]GC[CT]^C BbsI 1286 GAAGAC GAAGAC(2/6) BlnI 1168 CCTAGG C^CTAGG BpuAI 1286 GAAGAC GAAGAC(2/6) BsaBI 274 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 1127 GGTCTC GGTCTC(1/5) BseRI 289 GAGGAG GAGGAG(10/8) BsgI 826 GTGCAG GTGCAG(16/14) BspHI 449 TCATGA T^CATGA BspMI 1046 ACCTGC ACCTGC(4/8) BstAPI 532 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 86 TTCGAA TT^CGAA HinfI 262 GATTC G^A[ACGT]TC MamI 274 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MspA1I 536 CAGCTG C[AC]G^C[GT]G NspV 86 TTCGAA TT^CGAA PstI 931 CTGCAG CTGCA^G PvuII 536 CAGCTG CAG^CTG RcaI 449 TCATGA T^CATGA SfcI 931 CTGCAG C^T[GA][CT]AG SfuI 86 TTCGAA TT^CGAA TfiI 262 GATTC G^A[AT]TC XcmI 404 CCAAGACCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 578 GAAGCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AciI 1051 CCGC CCGC(-3/-1) 370 GCGG AcsI 21 AAATTT [GA]^AATT[CT] 780 GAATTT ApoI 21 AAATTT [GA]^AATT[CT] 780 GAATTT AspHI 989 GAGCAC G[AT]GC[AT]^C 1159 GAGCAC AvaI 1082 CCCGGG C^[CT]CG[GA]G 1281 CCCGGG AvaII 372 GGACC G^G[AT]CC 1020 GGACC BanI 868 GGTGCC G^G[CT][GA]CC 1043 GGCACC BpmI 949 CTGGAG CTGGAG(16/14) 1006 CTGGAG BsiHKAI 989 GAGCAC G[AT]GC[AT]^C 1159 GAGCAC BsmFI 517 GGGAC GGGAC(10/14) 123 GTCCC BsoBI 1082 CCCGGG C^[CT]CG[GA]G 1281 CCCGGG BsrFI 1022 ACCGGC [GA]^CCGG[CT] 1040 ACCGGC BsrI 514 ACTGG ACTGG(1/-1) 612 ACTGG BssSI 391 CACGAG CACGAG(-5/-1) 148 CTCGTG BstXI 410 CCAGCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 605 CCAGCCGACTGG BstYI 723 GGATCT [GA]^GATC[CT] 1102 GGATCC Cfr10I 1022 ACCGGC [GA]^CCGG[CT] 1040 ACCGGC DpnII 724 GATC ^GATC 1103 GATC EarI 426 CTCTTC CTCTTC(1/4) 238 GAAGAG HincII 189 GTCAAC GT[CT]^[GA]AC 379 GTTGAC HindII 189 GTCAAC GT[CT]^[GA]AC 379 GTTGAC Ksp632I 426 CTCTTC CTCTTC(1/4) 238 GAAGAG MboI 724 GATC ^GATC 1103 GATC MflI 723 GGATCT [GA]^GATC[CT] 1102 GGATCC MslI 199 CACAGTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 765 CATGGCTATG NdeII 724 GATC ^GATC 1103 GATC SapI 238 GAAGAGC GCTCTTC(1/4) 425 GCTCTTC Sau3AI 724 GATC ^GATC 1103 GATC SmaI 1082 CCCGGG CCC^GGG 1281 CCCGGG Tsp45I 254 GTGAC ^GT[GC]AC 481 GTGAC TspRI 201 CAGTG CA[GC]TG[ACGT][ACGT]^ 1190 CACTG XhoII 723 GGATCT [GA]^GATC[CT] 1102 GGATCC XmaCI 1082 CCCGGG C^CCGGG 1281 CCCGGG XmaI 1082 CCCGGG C^CCGGG 1281 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 53 enzymes) # ######################################### AluI AlwI AlwNI BalI BbvI BcnI BfaI BmyI BsaJI BsiYI BslI BsmAI Bsp1286I BssKI BstF5I BstNI Cac8I Cfr13I DdeI DraII EaeI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HpaII HphI ItaI MaeI MaeIII MboII MluNI MnlI MscI MseI MspI MvaI MwoI NciI NlaIII NlaIV Sau96I ScrFI SfaNI StyI TaqI Tru9I TseI Tsp509I TthHB8I