(Circular) (Six-base) MAPSORT of: pF1KE0268.seq Check: 5139 from: 1 to: 3773 pF1KE0268 3773 bp With 182 enzymes: SgfI * April 24, 2009 13:12 .. AccI GT'mk_AC Cuts at: 734 811 734 Size: 77 3696 Acc65I G'GTAC_C Cuts at: 789 789 Size: 3773 AclI AA'CG_TT Cuts at: 3613 3613 Size: 3773 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1598 2030 2777 3045 1598 Size: 432 747 268 2326 Fragments arranged by size: 2326 747 432 268 AflIII A'CryG_T Cuts at: 1125 2229 3206 1125 Size: 1104 977 1692 Fragments arranged by size: 1692 1104 977 AgeI A'CCGG_T Cuts at: 2161 2161 Size: 3773 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 307 307 Size: 3773 AlwNI CAG_nnn'CTG Cuts at: 1078 2645 1078 Size: 1567 2206 ApaI G_GGCC'C Cuts at: 145 532 145 Size: 387 3386 ApaLI G'TGCA_C Cuts at: 2543 2543 Size: 3773 ApoI r'AATT_y Cuts at: 777 3126 3382 777 Size: 2349 256 1168 Fragments arranged by size: 2349 1168 256 AseI AT'TA_AT Cuts at: 20 20 Size: 3773 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3773 AvaI C'yCGr_G Cuts at: 337 764 793 337 Size: 427 29 3317 Fragments arranged by size: 3317 427 29 BamHI G'GATC_C Cuts at: 798 798 Size: 3773 BanI G'GyrC_C Cuts at: 134 418 789 1203 1451 1486 134 Size: 284 371 414 248 35 2421 Fragments arranged by size: 2421 414 371 284 248 35 BanII G_rGCy'C Cuts at: 145 187 464 532 638 787 1817 145 Size: 42 277 68 106 149 1030 2101 Fragments arranged by size: 2101 1030 277 149 106 68 42 BbeI G_GCGC'C Cuts at: 138 1207 1455 138 Size: 1069 248 2456 Fragments arranged by size: 2456 1069 248 BbsI GAAGACnn'nnnn_ Cuts at: 515 515 Size: 3773 BbvCI CC'TCA_GC Cuts at: 673 673 Size: 3773 BciVI GTATCCnnnnn_n' Cuts at: 1671 2432 3773 1671 Size: 761 1341 1671 Fragments arranged by size: 1671 1341 761 BclI T'GATC_A Cuts at: 1293 1293 Size: 3773 BglI GCCn_nnn'nGGC Cuts at: 652 652 Size: 3773 BglII A'GATC_T Cuts at: 470 1288 3325 470 Size: 818 2037 918 Fragments arranged by size: 2037 918 818 BlpI GC'TnA_GC Cuts at: 885 885 Size: 3773 Bme1580I G_kGCm'C Cuts at: 145 532 1398 1491 2547 145 Size: 387 866 93 1056 1371 Fragments arranged by size: 1371 1056 866 387 93 BmgBI CAC'GTC Cuts at: 708 708 Size: 3773 BmrI ACTGGGnnnn_n' Cuts at: 985 1066 1401 3545 985 Size: 81 335 2144 1213 Fragments arranged by size: 2144 1213 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2140 2140 Size: 3773 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 189 685 189 Size: 496 3277 Bpu10I CC'TnA_GC Cuts at: 673 673 Size: 3773 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 634 949 1828 2320 2618 634 Size: 315 879 492 298 1789 Fragments arranged by size: 1789 879 492 315 298 BsaI GGTCTCn'nnnn_ Cuts at: 377 3477 377 Size: 3100 673 BsaAI yAC'GTr Cuts at: 1126 1126 Size: 3773 BsaBI GATnn'nnATC Cuts at: 6 1287 3324 6 Size: 1281 2037 455 Fragments arranged by size: 2037 1281 455 BsaHI Gr'CG_yC Cuts at: 135 239 584 1204 1452 2154 3650 135 Size: 104 345 620 248 702 1496 258 Fragments arranged by size: 1496 702 620 345 258 248 104 BsaWI w'CCGG_w Cuts at: 962 1483 2161 2435 2582 2993 962 Size: 521 678 274 147 411 1742 Fragments arranged by size: 1742 678 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 355 355 Size: 3773 BseYI C'CCAG_C Cuts at: 193 199 298 414 660 1198 1956 2533 3029 Size: 6 99 116 246 538 758 577 496 Cuts at: 3029 193 Size: 937 Fragments arranged by size: 937 758 577 538 496 246 116 99 6 BsiEI CG_ry'CG Cuts at: 104 324 618 1315 1361 2569 104 Size: 220 294 697 46 1208 1308 Fragments arranged by size: 1308 1208 697 294 220 46 BsiHKAI G_wGCw'C Cuts at: 260 464 638 787 1565 1755 2547 260 Size: 204 174 149 778 190 792 1486 Fragments arranged by size: 1486 792 778 204 190 174 149 BsmBI CGTCTCn'nnnn_ Cuts at: 541 541 Size: 3773 Bsp1286I G_dGCh'C Cuts at: 145 187 260 464 532 638 787 1398 1491 Size: 42 73 204 68 106 149 611 93 Cuts at: 1491 1565 1755 1817 2547 145 Size: 74 190 62 730 1371 Fragments arranged by size: 1371 730 611 204 190 149 106 93 74 73 68 62 42 BspEI T'CCGG_A Cuts at: 2993 2993 Size: 3773 BspHI T'CATG_A Cuts at: 2949 2949 Size: 3773 BspMI ACCTGCnnnn'nnnn_ Cuts at: 348 823 1037 1339 1720 348 Size: 475 214 302 381 2401 Fragments arranged by size: 2401 475 381 302 214 BsrBI CCG'CTC Cuts at: 50 2065 2119 3603 3768 50 Size: 2015 54 1484 165 55 Fragments arranged by size: 2015 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 302 1685 302 Size: 1383 2390 BsrFI r'CCGG_y Cuts at: 1771 1952 2161 1771 Size: 181 209 3383 Fragments arranged by size: 3383 209 181 BssSI C'ACGA_G Cuts at: 2044 2402 2044 Size: 358 3415 BstAPI GCAn_nnn'nTGC Cuts at: 1150 1150 Size: 3773 BstBI TT'CG_AA Cuts at: 740 2134 740 Size: 1394 2379 BstXI CCAn_nnnn'nTGG Cuts at: 456 540 456 Size: 84 3689 BstYI r'GATC_y Cuts at: 1 470 695 798 1265 1288 1623 1869 2881 Size: 469 225 103 467 23 335 246 1012 Cuts at: 2881 2967 2979 3325 1 Size: 86 12 346 449 Fragments arranged by size: 1012 469 467 449 346 335 246 225 103 86 23 12 Bsu36I CC'TnA_GG Cuts at: 146 146 Size: 3773 BtgI C'CryG_G Cuts at: 107 107 Size: 3773 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1723 1904 2067 1723 Size: 181 163 3429 Fragments arranged by size: 3429 181 163 BtsI GCAGTG_nn' Cuts at: 166 1016 166 Size: 850 2923 DraI TTT'AAA Cuts at: 772 772 Size: 3773 DrdI GACnn_nn'nnGTC Cuts at: 730 1479 2337 730 Size: 749 858 2166 Fragments arranged by size: 2166 858 749 EaeI y'GGCC_r Cuts at: 615 1358 1532 1923 1950 3295 615 Size: 743 174 391 27 1345 1093 Fragments arranged by size: 1345 1093 743 391 174 27 EagI C'GGCC_G Cuts at: 615 1358 615 Size: 743 3030 EarI CTCTTCn'nnn_ Cuts at: 344 737 1796 2006 344 Size: 393 1059 210 2111 Fragments arranged by size: 2111 1059 393 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 506 1981 2291 2437 3583 506 Size: 1475 310 146 1146 696 Fragments arranged by size: 1475 1146 696 310 146 EcoICRI GAG'CTC Cuts at: 462 636 785 462 Size: 174 149 3450 Fragments arranged by size: 3450 174 149 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 189 685 1598 2030 2777 3045 189 Size: 496 913 432 747 268 917 Fragments arranged by size: 917 913 747 496 432 268 EcoO109I rG'GnC_Cy Cuts at: 141 142 150 225 274 529 654 912 141 Size: 1 8 75 49 255 125 258 3002 Fragments arranged by size: 3002 258 255 125 75 49 8 1 EcoRI G'AATT_C Cuts at: 777 777 Size: 3773 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 171 519 1792 171 Size: 348 1273 2152 Fragments arranged by size: 2152 1273 348 FspI TGC'GCA Cuts at: 1554 1554 Size: 3773 HaeII r_GCGC'y Cuts at: 138 672 1207 1455 2477 3456 138 Size: 534 535 248 1022 979 455 Fragments arranged by size: 1022 979 535 534 455 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1615 1645 1647 1677 1615 Size: 30 2 30 3711 Fragments arranged by size: 3711 30 30 2 HincII GTy'rAC Cuts at: 735 812 735 Size: 77 3696 KasI G'GCGC_C Cuts at: 134 1203 1451 134 Size: 1069 248 2456 Fragments arranged by size: 2456 1069 248 KpnI G_GTAC'C Cuts at: 793 793 Size: 3773 MluI A'CGCG_T Cuts at: 3206 3206 Size: 3773 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2444 2628 2444 Size: 184 3589 MscI TGG'CCA Cuts at: 1534 1534 Size: 3773 MslI CAynn'nnrTG Cuts at: 454 625 1762 1889 454 Size: 171 1137 127 2338 Fragments arranged by size: 2338 1137 171 127 MspA1I CmG'CkG Cuts at: 681 884 1198 1558 2571 2816 681 Size: 203 314 360 1013 245 1638 Fragments arranged by size: 1638 1013 360 314 245 203 NaeI GCC'GGC Cuts at: 1954 1954 Size: 3773 NarI GG'CG_CC Cuts at: 135 1204 1452 135 Size: 1069 248 2456 Fragments arranged by size: 2456 1069 248 NcoI C'CATG_G Cuts at: 107 107 Size: 3773 NgoMIV G'CCGG_C Cuts at: 1952 1952 Size: 3773 NspI r_CATG'y Cuts at: 429 447 826 1326 2233 429 Size: 18 379 500 907 1969 Fragments arranged by size: 1969 907 500 379 18 PasI CC'CwG_GG Cuts at: 435 435 Size: 3773 PciI A'CATG_T Cuts at: 2229 2229 Size: 3773 PflMI CCAn_nnn'nTGG Cuts at: 708 708 Size: 3773 PfoI T'CCnGG_A Cuts at: 368 569 590 701 368 Size: 201 21 111 3440 Fragments arranged by size: 3440 201 111 21 PmeI GTTT'AAAC Cuts at: 772 772 Size: 3773 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2912 2944 2912 Size: 32 3741 PpuMI rG'GwC_Cy Cuts at: 150 225 150 Size: 75 3698 PspOMI G'GGCC_C Cuts at: 141 528 141 Size: 387 3386 PstI C_TGCA'G Cuts at: 359 820 1505 359 Size: 461 685 2627 Fragments arranged by size: 2627 685 461 PvuI CG_AT'CG Cuts at: 104 104 Size: 3773 PvuII CAG'CTG Cuts at: 1198 1558 1198 Size: 360 3413 RsrII CG'GwC_CG Cuts at: 1968 1968 Size: 3773 SacI G_AGCT'C Cuts at: 464 638 787 464 Size: 174 149 3450 Fragments arranged by size: 3450 174 149 SalI G'TCGA_C Cuts at: 733 810 733 Size: 77 3696 SanDI GG'GwC_CC Cuts at: 225 225 Size: 3773 SapI GCTCTTCn'nnn_ Cuts at: 344 1796 2006 344 Size: 1452 210 2111 Fragments arranged by size: 2111 1452 210 SbfI CC_TGCA'GG Cuts at: 820 820 Size: 3773 ScaI AGT'ACT Cuts at: 3290 3290 Size: 3773 SfcI C'TryA_G Cuts at: 33 355 816 1501 2494 2685 33 Size: 322 461 685 993 191 1121 Fragments arranged by size: 1121 993 685 461 322 191 SfiI GGCCn_nnn'nGGCC Cuts at: 652 652 Size: 3773 SfoI GGC'GCC Cuts at: 136 1205 1453 136 Size: 1069 248 2456 Fragments arranged by size: 2456 1069 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3773 SmaI CCC'GGG Cuts at: 795 795 Size: 3773 SmlI C'TyrA_G Cuts at: 649 928 1843 2335 2597 649 Size: 279 915 492 262 1825 Fragments arranged by size: 1825 915 492 279 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3773 SphI G_CATG'C Cuts at: 429 826 1326 429 Size: 397 500 2876 Fragments arranged by size: 2876 500 397 StyI C'CwwG_G Cuts at: 107 178 212 907 1261 107 Size: 71 34 695 354 2619 Fragments arranged by size: 2619 695 354 71 34 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 328 395 406 1356 1489 1948 2157 328 Size: 67 11 950 133 459 209 1944 Fragments arranged by size: 1944 950 459 209 133 67 11 TatI w'GTAC_w Cuts at: 3288 3288 Size: 3773 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3290 3322 3290 Size: 32 3741 Tth111I GACn'n_nGTC Cuts at: 1570 1570 Size: 3773 XbaI T'CTAG_A Cuts at: 804 804 Size: 3773 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1268 1268 Size: 3773 XmaI C'CCGG_G Cuts at: 793 793 Size: 3773 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AloI AscI AvrII BaeI BcgI BfrBI BmtI BsaXI BsgI BsiWI BsmI BsrGI BssHII BstEII BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PmlI PshAI PsiI PspXI PsrI SacII SexAI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI