Restriction map for pF1KE0265
##################################
# NO CUT ENZYMES (  167 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AflII       AflIII      AgeI        Alw44I      AlwNI       
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AseI        AsnI        Asp700I     Asp718I     AspHI       
AspI        AvrII       BamHI       BanI        BanII       
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BclI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        BmyI        Bpu1102I    BsaAI       
BsaBI       BsaI        BsaWI       BseAI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsmAI       
BsmBI       BsmI        Bsp1286I    Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrGI       BsrI        BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstXI       BstZ17I     
Bsu36I      CelII       ClaI        CpoI        DraI        
DraII       DraIII      DrdI        DsaI        EagI        
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        HaeII       HincII      
HindII      HindIII     HpaI        KasI        KpnI        
Ksp632I     KspI        MaeI        MaeII       MamI        
MfeI        MluI        MroI        MseI        MslI        
MunI        NarI        NcoI        NdeI        NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        TfiI        Tru9I       Tsp509I     TspRI       
Tth111I     Van91I      XbaI        XcmI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AccI          148  GTCTAC               GT^[AC][GT]AC
AcyI          319  GGCGTC               G[GA]^CG[CT]C
AfaI          343  GTAC                 GT^AC
AhdI          360  GACGGAGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI          186  GATCC                GGATC(4/5)
AscI          139  GGCGCGCC             GG^CGCGCC
AspEI         360  GACGGAGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII         162  GGTCC                G^G[AT]CC
AviII         104  TGCGCA               TGC^GCA
BalI          257  TGGCCA               TGG^CCA
BcnI           78  CCCGG                CC^[GC]GG
BsaHI         319  GGCGTC               G[GA]^CG[CT]C
BsrFI         136  GCCGGC               [GA]^CCGG[CT]
BssHII        140  GCGCGC               G^CGCGC
BstAPI         93  GCATCGCTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI         185  AGATCC               [GA]^GATC[CT]
Cfr10I        136  GCCGGC               [GA]^CCGG[CT]
DdeI          353  CTAAG                C^T[ACGT]AG
DpnII         186  GATC                 ^GATC
EaeI          257  TGGCCA               [CT]^GGCC[GA]
Eam1105I      360  GACGGAGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I        38  CCAAGG               C^C[AT][AT]GG
FspI          104  TGCGCA               TGC^GCA
Hin1I         319  GGCGTC               G[GA]^CG[CT]C
HinfI         366  GAGTC                G^A[ACGT]TC
MboI          186  GATC                 ^GATC
MflI          185  AGATCC               [GA]^GATC[CT]
MluNI         257  TGGCCA               TGG^CCA
MscI          257  TGGCCA               TGG^CCA
MspA1I        253  CAGCTG               C[AC]G^C[GT]G
NaeI          136  GCCGGC               GCC^GGC
NciI           78  CCCGG                CC^[GC]GG
NdeII         186  GATC                 ^GATC
NgoMIV        136  GCCGGC               G^CCGGC
NlaIII        153  CATG                 CATG^
NlaIV         133  GGGGCC               GG[ACGT]^[ACGT]CC
PaeR7I        166  CTCGAG               C^TCGAG
PleI          366  GAGTC                GAGTC(4/5)
PvuII         253  CAGCTG               CAG^CTG
RsaI          343  GTAC                 GT^AC
Sau3AI        186  GATC                 ^GATC
SmlI          166  CTCGAG               C^T[CT][GA]AG
StyI           38  CCAAGG               C^C[AT][AT]GG
TaqI          167  TCGA                 T^CGA
TthHB8I       167  TCGA                 T^CGA
XhoI          166  CTCGAG               C^TCGAG
XhoII         185  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

AvaI          166  CTCGAG               C^[CT]CG[GA]G
              212  CTCGGG              
BbsI          225  GAAGAC               GAAGAC(2/6)
              357  GAAGAC              
BpmI          250  CTCCAG               CTGGAG(16/14)
              190  CTGGAG              
BpuAI         225  GAAGAC               GAAGAC(2/6)
              357  GAAGAC              
BsmFI         215  GGGAC                GGGAC(10/14)
               57  GTCCC               
BsoBI         166  CTCGAG               C^[CT]CG[GA]G
              212  CTCGGG              
HapII          79  CCGG                 C^CGG
              137  CCGG                
HgaI          228  GACGC                GACGC(5/10)
              320  GCGTC               
HpaII          79  CCGG                 C^CGG
              137  CCGG                
MboII         225  GAAGA                GAAGA(8/7)
              357  GAAGA               
MspI           79  CCGG                 C^CGG
              137  CCGG                
SfaNI          93  GCATC                GCATC(5/9)
              233  GCATC               


#########################################
# 3 OR MORE CUT ENZYMES (   32 enzymes) #
#########################################

AccII       AciI        AluI        BbvI        BsaJI       
BsiYI       BslI        BssKI       BstF5I      BstNI       
BstUI       Cac8I       CfoI        Cfr13I      EcoRII      
FauI        Fnu4HI      FokI        HaeIII      HhaI        
HinP1I      HphI        ItaI        MaeIII      MnlI        
MvaI        MvnI        MwoI        Sau96I      ScrFI       
TseI        Tsp45I      
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