Restriction map for pF1KE0264
##################################
# NO CUT ENZYMES (  129 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       AspHI       AspI        AvaII       
AviII       AvrII       BamHI       BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaWI       BseAI       BsiEI       
BsiHKAI     BsiWI       BsmBI       BspDI       BspEI       
BsrBI       BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstYI       BstZ17I     Bsu36I      
CelII       ClaI        CpoI        DraII       DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT14I     EcoT22I     FbaI        
FseI        FspI        HgaI        HindIII     HpaI        
KpnI        KspI        MaeII       MamI        MflI        
MluI        MroI        NcoI        NdeI        NheI        
NotI        NsiI        NspV        PacI        PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshBI       Psp1406I    PvuI        RsrII       
SacI        SacII       SalI        SapI        ScaI        
SexAI       SfiI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        SspI        StuI        
StyI        SwaI        Tth111I     Van91I      XbaI        
XcmI        XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AccI          902  GTAGAC               GT^[AC][GT]AC
AcsI         1035  AAATTT               [GA]^AATT[CT]
AcyI           21  GGCGCC               G[GA]^CG[CT]C
AflII         759  CTTAAG               C^TTAAG
AflIII         94  ACATGT               A^C[GA][CT]GT
AlwI          450  GGATC                GGATC(4/5)
ApoI         1035  AAATTT               [GA]^AATT[CT]
Asp700I       139  GAAGGTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI          238  CTCGAG               C^[CT]CG[GA]G
BanI           21  GGCGCC               G^G[CT][GA]CC
BanII         338  GAGCCC               G[GA]GC[CT]^C
BbeI           21  GGCGCC               GGCGC^C
BcnI          516  CCGGG                CC^[GC]GG
BfaI          671  CTAG                 C^TAG
BfrI          759  CTTAAG               C^TTAAG
BsaHI          21  GGCGCC               G[GA]^CG[CT]C
BsaI           81  GAGACC               GGTCTC(1/5)
BseRI        1105  CTCCTC               GAGGAG(10/8)
BsmI          104  GCATTC               GAATGC(1/-1)
BsoBI         238  CTCGAG               C^[CT]CG[GA]G
Bsp1407I      278  TGTACA               T^GTACA
BspHI        1187  TCATGA               T^CATGA
BspLU11I       94  ACATGT               A^CATGT
BspMI         705  ACCTGC               ACCTGC(4/8)
BsrFI         955  GCCGGC               [GA]^CCGG[CT]
BsrGI         278  TGTACA               T^GTACA
BstAPI       1091  GCAAGCAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I        955  GCCGGC               [GA]^CCGG[CT]
Cfr13I       1137  GGGCC                G^G[ACGT]CC
EarI         1112  GAAGAG               CTCTTC(1/4)
HaeII          21  GGCGCC               [GA]GCGC^[CT]
Hin1I          21  GGCGCC               G[GA]^CG[CT]C
KasI           21  GGCGCC               G^GCGCC
Ksp632I      1112  GAAGAG               CTCTTC(1/4)
MaeI          671  CTAG                 C^TAG
MfeI          713  CAATTG               C^AATTG
MunI          713  CAATTG               C^AATTG
NaeI          955  GCCGGC               GCC^GGC
NarI           21  GGCGCC               GG^CGCC
NciI          516  CCGGG                CC^[GC]GG
NgoMIV        955  GCCGGC               G^CCGGC
NruI          981  TCGCGA               TCG^CGA
NspI           94  ACATGT               [GA]CATG^[CT]
PaeR7I        238  CTCGAG               C^TCGAG
PshAI        1080  GACTGTCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII         395  CAGCTG               CAG^CTG
RcaI         1187  TCATGA               T^CATGA
Sau96I       1137  GGGCC                G^G[ACGT]CC
SbfI          526  CCTGCAGG             CCTGCA^GG
SfoI           21  GGCGCC               GGC^GCC
Sse8387I      526  CCTGCAGG             CCTGCA^GG
SspBI         278  TGTACA               T^GTACA
XhoI          238  CTCGAG               C^TCGAG
XmnI          139  GAAGGTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

BmyI          338  GAGCCC               G[AGT]GC[ACT]^C
             1098  GTGCCC              
BsgI          707  CTGCAC               GTGCAG(16/14)
              370  GTGCAG              
BsiYI          33  CCTGCTGCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              500  CCCCCAACTGG         
BslI           33  CCTGCTGCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              500  CCCCCAACTGG         
BsmAI          81  GAGAC                GTCTC(1/5)
             1006  GTCTC               
BsmFI         913  GGGAC                GGGAC(10/14)
               72  GTCCC               
Bsp1286I      338  GAGCCC               G[AGT]GC[ACT]^C
             1098  GTGCCC              
BsrDI         401  GCAATG               GCAATG(2/0)
             1239  GCAATG              
BstF5I        273  GGATG                GGATG(2/0)
             1221  GGATG               
BstXI         288  CCATGCCAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              300  CCAGCGCATTGG        
DdeI          722  CTGAG                C^T[ACGT]AG
             1108  CTCAG               
DpnII         451  GATC                 ^GATC
             1011  GATC                
DraI          493  TTTAAA               TTT^AAA
              867  TTTAAA              
DsaI          860  CCACGG               C^C[GA][CT]GG
              878  CCGTGG              
FauI          653  GCGGG                CCCGC(4/6)
             1054  GCGGG               
FokI          273  GGATG                GGATG(9/13)
             1221  GGATG               
HincII        661  GTCAAC               GT[CT]^[GA]AC
              890  GTCAAC              
HindII        661  GTCAAC               GT[CT]^[GA]AC
              890  GTCAAC              
MboI          451  GATC                 ^GATC
             1011  GATC                
MspA1I        395  CAGCTG               C[AC]G^C[GT]G
              425  CCGCTG              
NdeII         451  GATC                 ^GATC
             1011  GATC                
NlaIV          21  GGCGCC               GG[ACGT]^[ACGT]CC
              337  GGAGCC              
PstI          527  CTGCAG               CTGCA^G
              770  CTGCAG              
Sau3AI        451  GATC                 ^GATC
             1011  GATC                
SmlI          238  CTCGAG               C^T[CT][GA]AG
              759  CTTAAG              
TaqI          239  TCGA                 T^CGA
             1009  TCGA                
TthHB8I       239  TCGA                 T^CGA
             1009  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   48 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        BalI        
BbvI        BpmI        BsaJI       BsrI        BssKI       
BstNI       BstUI       Cac8I       CfoI        EaeI        
EcoRII      Fnu4HI      HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
MaeIII      MboII       MluNI       MnlI        MscI        
MseI        MslI        MspI        MvaI        MvnI        
MwoI        NlaIII      PleI        RsaI        ScrFI       
SfaNI       SfcI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       
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