(Circular) (Six-base) MAPSORT of: pF1KE0261.seq Check: 6334 from: 1 to: 4238 pF1KE0261 4238 bp With 182 enzymes: SgfI * April 24, 2009 13:12 .. AccI GT'mk_AC Cuts at: 1276 1276 Size: 4238 Acc65I G'GTAC_C Cuts at: 1254 1254 Size: 4238 AclI AA'CG_TT Cuts at: 4078 4078 Size: 4238 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 277 455 2063 2495 3242 3510 277 Size: 178 1608 432 747 268 1005 Fragments arranged by size: 1608 1005 747 432 268 178 AflIII A'CryG_T Cuts at: 1590 2694 3671 1590 Size: 1104 977 2157 Fragments arranged by size: 2157 1104 977 AgeI A'CCGG_T Cuts at: 2626 2626 Size: 4238 AlwNI CAG_nnn'CTG Cuts at: 781 1543 3110 781 Size: 762 1567 1909 Fragments arranged by size: 1909 1567 762 ApaI G_GGCC'C Cuts at: 699 699 Size: 4238 ApaLI G'TGCA_C Cuts at: 3008 3008 Size: 4238 ApoI r'AATT_y Cuts at: 1242 3591 3847 1242 Size: 2349 256 1633 Fragments arranged by size: 2349 1633 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4238 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4238 AvaI C'yCGr_G Cuts at: 1258 1258 Size: 4238 BamHI G'GATC_C Cuts at: 1263 1263 Size: 4238 BanI G'GyrC_C Cuts at: 1010 1254 1668 1916 1951 1010 Size: 244 414 248 35 3297 Fragments arranged by size: 3297 414 248 244 35 BanII G_rGCy'C Cuts at: 326 699 1252 2282 326 Size: 373 553 1030 2282 Fragments arranged by size: 2282 1030 553 373 BbeI G_GCGC'C Cuts at: 1672 1920 1672 Size: 248 3990 BbvCI CC'TCA_GC Cuts at: 167 167 Size: 4238 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 192 226 192 Size: 34 4204 BciVI GTATCCnnnnn_n' Cuts at: 2136 2897 4238 2136 Size: 761 1341 2136 Fragments arranged by size: 2136 1341 761 BclI T'GATC_A Cuts at: 1758 1758 Size: 4238 BglI GCCn_nnn'nGGC Cuts at: 667 667 Size: 4238 BglII A'GATC_T Cuts at: 1753 3790 1753 Size: 2037 2201 BlpI GC'TnA_GC Cuts at: 821 1350 821 Size: 529 3709 Bme1580I G_kGCm'C Cuts at: 699 1863 1956 3012 699 Size: 1164 93 1056 1925 Fragments arranged by size: 1925 1164 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 371 1450 1531 1866 4010 371 Size: 1079 81 335 2144 599 Fragments arranged by size: 2144 1079 599 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2605 2605 Size: 4238 Bpu10I CC'TnA_GC Cuts at: 167 167 Size: 4238 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 182 1414 2293 2785 3083 182 Size: 1232 879 492 298 1337 Fragments arranged by size: 1337 1232 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 394 882 3942 394 Size: 488 3060 690 Fragments arranged by size: 3060 690 488 BsaAI yAC'GTr Cuts at: 1591 1591 Size: 4238 BsaBI GATnn'nnATC Cuts at: 6 1752 3789 6 Size: 1746 2037 455 Fragments arranged by size: 2037 1746 455 BsaHI Gr'CG_yC Cuts at: 1669 1917 2619 4115 1669 Size: 248 702 1496 1792 Fragments arranged by size: 1792 1496 702 248 BsaWI w'CCGG_w Cuts at: 1427 1948 2626 2900 3047 3458 1427 Size: 521 678 274 147 411 2207 Fragments arranged by size: 2207 678 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 184 775 1036 1102 1165 1168 184 Size: 591 261 66 63 3 3254 Fragments arranged by size: 3254 591 261 66 63 3 BseYI C'CCAG_C Cuts at: 127 1663 2421 2998 3494 127 Size: 1536 758 577 496 871 Fragments arranged by size: 1536 871 758 577 496 BsiEI CG_ry'CG Cuts at: 104 216 1780 1826 3034 104 Size: 112 1564 46 1208 1308 Fragments arranged by size: 1564 1308 1208 112 46 BsiHKAI G_wGCw'C Cuts at: 326 1252 2030 2220 3012 326 Size: 926 778 190 792 1552 Fragments arranged by size: 1552 926 792 778 190 BsmBI CGTCTCn'nnnn_ Cuts at: 679 679 Size: 4238 Bsp1286I G_dGCh'C Cuts at: 326 699 1252 1863 1956 2030 2220 2282 3012 Size: 373 553 611 93 74 190 62 730 Cuts at: 3012 326 Size: 1552 Fragments arranged by size: 1552 730 611 553 373 190 93 74 62 BspEI T'CCGG_A Cuts at: 3458 3458 Size: 4238 BspHI T'CATG_A Cuts at: 3414 3414 Size: 4238 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1221 1288 1502 1804 2185 1221 Size: 67 214 302 381 3274 Fragments arranged by size: 3274 381 302 214 67 BsrBI CCG'CTC Cuts at: 50 122 2530 2584 4068 4233 50 Size: 72 2408 54 1484 165 55 Fragments arranged by size: 2408 1484 165 72 55 54 BsrDI GCAATG_nn' Cuts at: 138 2150 138 Size: 2012 2226 BsrFI r'CCGG_y Cuts at: 2236 2417 2626 2236 Size: 181 209 3848 Fragments arranged by size: 3848 209 181 BssSI C'ACGA_G Cuts at: 2509 2867 2509 Size: 358 3880 BstAPI GCAn_nnn'nTGC Cuts at: 1227 1615 1227 Size: 388 3850 BstBI TT'CG_AA Cuts at: 2599 2599 Size: 4238 BstXI CCAn_nnnn'nTGG Cuts at: 691 691 Size: 4238 BstYI r'GATC_y Cuts at: 1 1263 1730 1753 2088 2334 3346 3432 3444 Size: 1262 467 23 335 246 1012 86 12 Cuts at: 3444 3790 1 Size: 346 449 Fragments arranged by size: 1262 1012 467 449 346 335 246 86 23 12 Bsu36I CC'TnA_GG Cuts at: 982 982 Size: 4238 BtgI C'CryG_G Cuts at: 115 115 Size: 4238 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2188 2369 2532 2188 Size: 181 163 3894 Fragments arranged by size: 3894 181 163 BtsI GCAGTG_nn' Cuts at: 516 1080 1481 516 Size: 564 401 3273 Fragments arranged by size: 3273 564 401 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 132 167 132 Size: 35 4203 DraI TTT'AAA Cuts at: 1237 1237 Size: 4238 DrdI GACnn_nn'nnGTC Cuts at: 1944 2802 1944 Size: 858 3380 EaeI y'GGCC_r Cuts at: 213 341 1823 1997 2388 2415 3760 213 Size: 128 1482 174 391 27 1345 691 Fragments arranged by size: 1482 1345 691 391 174 128 27 EagI C'GGCC_G Cuts at: 213 1823 213 Size: 1610 2628 EarI CTCTTCn'nnn_ Cuts at: 331 997 1013 1129 2261 2471 331 Size: 666 16 116 1132 210 2098 Fragments arranged by size: 2098 1132 666 210 116 16 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2446 2756 2902 4048 2446 Size: 310 146 1146 2636 Fragments arranged by size: 2636 1146 310 146 EcoICRI GAG'CTC Cuts at: 324 1250 324 Size: 926 3312 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 277 455 2063 2495 3242 3510 277 Size: 178 1608 432 747 268 1005 Fragments arranged by size: 1608 1005 747 432 268 178 EcoNI CCTnn'n_nnAGG Cuts at: 394 394 Size: 4238 EcoO109I rG'GnC_Cy Cuts at: 687 695 696 1377 687 Size: 8 1 681 3548 Fragments arranged by size: 3548 681 8 1 EcoRI G'AATT_C Cuts at: 1242 1242 Size: 4238 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 447 2257 447 Size: 1810 2428 FspI TGC'GCA Cuts at: 2019 2019 Size: 4238 HaeII r_GCGC'y Cuts at: 1672 1920 2942 3921 1672 Size: 248 1022 979 1989 Fragments arranged by size: 1989 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 2080 2110 2112 2142 2080 Size: 30 2 30 4176 Fragments arranged by size: 4176 30 30 2 HincII GTy'rAC Cuts at: 1277 1277 Size: 4238 HindIII A'AGCT_T Cuts at: 631 631 Size: 4238 KasI G'GCGC_C Cuts at: 1668 1916 1668 Size: 248 3990 KpnI G_GTAC'C Cuts at: 1258 1258 Size: 4238 MluI A'CGCG_T Cuts at: 3671 3671 Size: 4238 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2909 3093 2909 Size: 184 4054 MscI TGG'CCA Cuts at: 1999 1999 Size: 4238 MslI CAynn'nnrTG Cuts at: 2227 2354 2227 Size: 127 4111 MspA1I CmG'CkG Cuts at: 117 177 1349 1663 2023 3036 3281 117 Size: 60 1172 314 360 1013 245 1074 Fragments arranged by size: 1172 1074 1013 360 314 245 60 NaeI GCC'GGC Cuts at: 2419 2419 Size: 4238 NarI GG'CG_CC Cuts at: 1669 1917 1669 Size: 248 3990 NdeI CA'TA_TG Cuts at: 1107 1107 Size: 4238 NgoMIV G'CCGG_C Cuts at: 2417 2417 Size: 4238 NspI r_CATG'y Cuts at: 820 1230 1291 1791 2698 820 Size: 410 61 500 907 2360 Fragments arranged by size: 2360 907 500 410 61 PasI CC'CwG_GG Cuts at: 691 691 Size: 4238 PciI A'CATG_T Cuts at: 2694 2694 Size: 4238 PmeI GTTT'AAAC Cuts at: 1237 1237 Size: 4238 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3377 3409 3377 Size: 32 4206 PpuMI rG'GwC_Cy Cuts at: 687 687 Size: 4238 PsiI TTA'TAA Cuts at: 413 413 Size: 4238 PspOMI G'GGCC_C Cuts at: 695 695 Size: 4238 PstI C_TGCA'G Cuts at: 511 710 930 1071 1285 1970 511 Size: 199 220 141 214 685 2779 Fragments arranged by size: 2779 685 220 214 199 141 PvuI CG_AT'CG Cuts at: 104 104 Size: 4238 PvuII CAG'CTG Cuts at: 177 1663 2023 177 Size: 1486 360 2392 Fragments arranged by size: 2392 1486 360 RsrII CG'GwC_CG Cuts at: 2433 2433 Size: 4238 SacI G_AGCT'C Cuts at: 326 1252 326 Size: 926 3312 SacII CC_GC'GG Cuts at: 118 118 Size: 4238 SalI G'TCGA_C Cuts at: 1275 1275 Size: 4238 SapI GCTCTTCn'nnn_ Cuts at: 331 2261 2471 331 Size: 1930 210 2098 Fragments arranged by size: 2098 1930 210 SbfI CC_TGCA'GG Cuts at: 1285 1285 Size: 4238 ScaI AGT'ACT Cuts at: 3755 3755 Size: 4238 SfcI C'TryA_G Cuts at: 33 507 706 773 926 1067 1281 1966 2959 Size: 474 199 67 153 141 214 685 993 Cuts at: 2959 3150 33 Size: 191 1121 Fragments arranged by size: 1121 993 685 474 214 199 191 153 141 67 SfoI GGC'GCC Cuts at: 1670 1918 1670 Size: 248 3990 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4238 SmaI CCC'GGG Cuts at: 1260 1260 Size: 4238 SmlI C'TyrA_G Cuts at: 197 1393 2308 2800 3062 197 Size: 1196 915 492 262 1373 Fragments arranged by size: 1373 1196 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4238 SphI G_CATG'C Cuts at: 1230 1291 1791 1230 Size: 61 500 3677 Fragments arranged by size: 3677 500 61 StuI AGG'CCT Cuts at: 209 661 209 Size: 452 3786 StyI C'CwwG_G Cuts at: 293 1372 1726 293 Size: 1079 354 2805 Fragments arranged by size: 2805 1079 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 824 1821 1954 2413 2622 824 Size: 997 133 459 209 2440 Fragments arranged by size: 2440 997 459 209 133 TatI w'GTAC_w Cuts at: 375 3753 375 Size: 3378 860 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3755 3787 3755 Size: 32 4206 Tth111I GACn'n_nGTC Cuts at: 139 2035 139 Size: 1896 2342 XbaI T'CTAG_A Cuts at: 1269 1269 Size: 4238 XcmI CCAnnnn_n'nnnnTGG Cuts at: 351 1733 351 Size: 1382 2856 XmaI C'CCGG_G Cuts at: 1258 1258 Size: 4238 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV NspI PasI PciI PmeI PpiI PpuMI PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AlfI AloI AscI AvrII BaeI BbsI BfrBI BmgBI BmtI BpmI BsaXI BsgI BsiWI BsmI BsrGI BssHII BstEII BstZ17I ClaI DraIII EcoRV FseI FspAI HpaI MfeI NcoI NheI NotI NruI NsiI PacI PflMI PfoI PmlI PshAI PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI