Restriction map for pF1KE0248
##################################
# NO CUT ENZYMES (  181 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AcsI        AflII       AgeI        AhdI        Alw44I      
AlwI        AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AvaI        AviII       
AvrII       BalI        BamHI       BanI        BbeI        
BbvCI       BcgI        BciVI       BclI        BfrI        
BglI        BglII       BlnI        BlpI        BpmI        
Bpu1102I    BsaBI       BsgI        BsiEI       BsiWI       
BsmFI       BsmI        BsoBI       Bsp1407I    BspDI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstXI       BstYI       BstZ17I     Bsu36I      CelII       
CfoI        Cfr10I      ClaI        CpoI        DdeI        
DpnII       DraI        DraII       DraIII      DsaI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT14I     EcoT22I     FauI        FbaI        
FseI        FspI        HaeII       HhaI        HinP1I      
HincII      HindII      HindIII     HinfI       HpaI        
HphI        KasI        KpnI        KspI        MaeIII      
MamI        MboI        MfeI        MflI        MluI        
MluNI       MscI        MseI        MslI        MunI        
MvnI        NaeI        NarI        NcoI        NdeI        
NdeII       NgoMIV      NlaIII      NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PinAI       PleI        PmeI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
RcaI        RsrII       SacII       SalI        SapI        
Sau3AI      SbfI        ScaI        SexAI       SfcI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
TfiI        Tru9I       Tsp45I      Tth111I     XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AcyI          112  GGCGTC               G[GA]^CG[CT]C
AfaI          327  GTAC                 GT^AC
AflIII        164  ACGTGT               A^C[GA][CT]GT
AlwNI          10  CAGCAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AvaII         363  GGACC                G^G[AT]CC
BanII          58  GAGCTC               G[GA]GC[CT]^C
BbrPI         163  CACGTG               CAC^GTG
BcnI          109  CCCGG                CC^[GC]GG
BfaI          205  CTAG                 C^TAG
BsaAI         163  CACGTG               [CT]AC^GT[GA]
BsaHI         112  GGCGTC               G[GA]^CG[CT]C
BsaI           76  GAGACC               GGTCTC(1/5)
BsmBI         114  CGTCTC               CGTCTC(1/5)
BsrI          288  ACTGG                ACTGG(1/-1)
BstNI         255  CCTGG                CC^[AT]GG
Cfr13I        363  GGACC                G^G[ACGT]CC
DrdI          106  GACCCCGGCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI           17  TGGCCG               [CT]^GGCC[GA]
EcoRII        255  CCTGG                ^CC[AT]GG
HaeIII         18  GGCC                 GG^CC
Hin1I         112  GGCGTC               G[GA]^CG[CT]C
MaeI          205  CTAG                 C^TAG
MspA1I         13  CAGCTG               C[AC]G^C[GT]G
MvaI          255  CCTGG                CC^[AT]GG
NciI          109  CCCGG                CC^[GC]GG
NheI          204  GCTAGC               G^CTAGC
NlaIV         351  GGAGCC               GG[ACGT]^[ACGT]CC
PflMI           9  CCAGCAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI         163  CACGTG               CAC^GTG
PmlI          163  CACGTG               CAC^GTG
PvuII          13  CAGCTG               CAG^CTG
RsaI          327  GTAC                 GT^AC
SacI           58  GAGCTC               GAGCT^C
Sau96I        363  GGACC                G^G[ACGT]CC
TaqI           56  TCGA                 T^CGA
Tsp509I       157  AATT                 ^AATT
TspRI         330  CAGTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I        56  TCGA                 T^CGA
Van91I          9  CCAGCAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AccIII        135  TCCGGA               T^CCGGA
              297  TCCGGA              
AspHI          39  GTGCTC               G[AT]GC[AT]^C
               58  GAGCTC              
BbsI          237  GAAGAC               GAAGAC(2/6)
              265  GTCTTC              
BmyI           39  GTGCTC               G[AGT]GC[ACT]^C
               58  GAGCTC              
BpuAI         237  GAAGAC               GAAGAC(2/6)
              265  GTCTTC              
BsaJI          71  CCGAGG               C^C[ACGT][ACGT]GG
              108  CCCCGG              
BsaWI         135  TCCGGA               [AT]^CCGG[AT]
              297  TCCGGA              
BseAI         135  TCCGGA               T^CCGGA
              297  TCCGGA              
BsiHKAI        39  GTGCTC               G[AT]GC[AT]^C
               58  GAGCTC              
BsmAI          76  GAGAC                GTCTC(1/5)
              115  GTCTC               
Bsp1286I       39  GTGCTC               G[AGT]GC[ACT]^C
               58  GAGCTC              
BspEI         135  TCCGGA               T^CCGGA
              297  TCCGGA              
BssKI         109  CCCGG                ^CC[ACGT]GG
              255  CCTGG               
BstF5I         89  GGATG                GGATG(2/0)
              300  GGATG               
EarI          322  GAAGAG               CTCTTC(1/4)
              346  GAAGAG              
FokI           89  GGATG                GGATG(9/13)
              300  GGATG               
HgaI          240  GACGC                GACGC(5/10)
              113  GCGTC               
Ksp632I       322  GAAGAG               CTCTTC(1/4)
              346  GAAGAG              
MroI          135  TCCGGA               T^CCGGA
              297  TCCGGA              
ScrFI         109  CCCGG                CC^[ACGT]GG
              255  CCTGG               
SfaNI         301  GATGC                GCATC(5/9)
              313  GATGC               


#########################################
# 3 OR MORE CUT ENZYMES (   17 enzymes) #
#########################################

AciI        AluI        BbvI        BseRI       BsiYI       
BslI        Cac8I       Fnu4HI      HapII       HpaII       
ItaI        MaeII       MboII       MnlI        MspI        
MwoI        TseI        
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