Restriction map for
pF1KE0247
##################################
# NO CUT ENZYMES ( 150 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AvaI AviII AvrII
BanI BbeI BbrPI BbsI BcgI
BciVI BcnI BfrI BglI BlnI
BlpI Bpu1102I BpuAI BsaAI BsaHI
BsaI BsaWI BseAI BsiEI BsiHKAI
BsiWI BsmBI BsoBI Bsp1407I BspEI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstXI BstZ17I CelII
CfoI Cfr10I CpoI DraI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRV
EcoT14I FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HpaI
HpaII KasI KpnI KspI MaeII
MfeI MluI MroI MspI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NspI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PstI PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StyI SwaI Van91I
XbaI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AfaI 1292 GTAC GT^AC
AocI 1460 CCTTAGG CC^T[ACGT]AGG
AspI 53 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 978 TGGCCA TGG^CCA
BamHI 675 GGATCC G^GATCC
BanII 658 GGGCTC G[GA]GC[CT]^C
BclI 234 TGATCA T^GATCA
BglII 225 AGATCT A^GATCT
BmyI 658 GGGCTC G[AGT]GC[ACT]^C
BpmI 328 CTGGAG CTGGAG(16/14)
BsaBI 451 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 1455 CTGCAC GTGCAG(16/14)
Bsp1286I 658 GGGCTC G[AGT]GC[ACT]^C
BspDI 751 ATCGAT AT^CGAT
BspHI 1077 TCATGA T^CATGA
Bsu36I 1460 CCTTAGG CC^T[ACGT]AGG
ClaI 751 ATCGAT AT^CGAT
EaeI 978 TGGCCA [CT]^GGCC[GA]
EarI 1310 GAAGAG CTCTTC(1/4)
Eco81I 1460 CCTTAGG CC^T[ACGT]AGG
EcoT22I 418 ATGCAT ATGCA^T
FauI 37 CCCGC CCCGC(4/6)
FbaI 234 TGATCA T^GATCA
HincII 1359 GTCAAC GT[CT]^[GA]AC
HindII 1359 GTCAAC GT[CT]^[GA]AC
HindIII 545 AAGCTT A^AGCTT
Ksp632I 1310 GAAGAG CTCTTC(1/4)
MamI 451 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 978 TGGCCA TGG^CCA
MscI 978 TGGCCA TGG^CCA
MslI 872 CATCAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 176 CAGCTG C[AC]G^C[GT]G
NsiI 418 ATGCAT ATGCA^T
PflFI 53 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 581 GGGACCT [GA]G^G[AT]CC[CT]
PvuII 176 CAGCTG CAG^CTG
RcaI 1077 TCATGA T^CATGA
RsaI 1292 GTAC GT^AC
SfcI 1319 CTGTAG C^T[GA][CT]AG
SmlI 586 CTTGAG C^T[CT][GA]AG
StuI 1603 AGGCCT AGG^CCT
Tth111I 53 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1547 CCAATTTCATATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 10 enzymes) #
###################################
AciI 38 CCGC CCGC(-3/-1)
1734 CCGC
AlwNI 469 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
717 CAGTGGCTG
AvaII 582 GGACC G^G[AT]CC
1196 GGACC
BseRI 987 CTCCTC GAGGAG(10/8)
1498 GAGGAG
BsiYI 61 CCTGATGTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1382 CCAAATCTAGG
BslI 61 CCTGATGTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1382 CCAAATCTAGG
BspMI 319 GCAGGT ACCTGC(4/8)
1492 GCAGGT
DraII 356 AGGGCCT [GA]G^G[ACGT]CC[CT]
581 GGGACCT
EcoO109I 356 AGGGCCT [GA]G^G[ACGT]CC[CT]
581 GGGACCT
PshAI 268 GACCAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1567 GACTTAGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AcsI AluI AlwI ApoI BbvCI
BbvI BfaI BsaJI BsmAI BsmFI
BsmI BsrI BssKI BstAPI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII EcoRI EcoRII Fnu4HI FokI
HaeIII HinfI HphI ItaI MaeI
MaeIII MboI MboII MflI MnlI
MseI MvaI MwoI NdeII NlaIII
NlaIV PleI Sau3AI Sau96I ScrFI
SfaNI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII