Restriction map for pF1KE0247
 (Linear) (Six-base) MAPSORT of: KIEE0247.seq  Check: 9679  from: 1  to: 1851



KIEE0247.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:12  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1483    1851 
   Size:       1483     368
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       2      32      89     158     617     692    1094    1145 
   Size:          2      30      57      69     459      75     402      51

Cuts at:   1145    1320    1851 
   Size:        175     531

  Fragments arranged by size: 

                 531     459     402     175      75      69      57      51
                  30       2
 
AlwNI CAG_nnn'CTG

Cuts at:      0     474     722    1851 
   Size:        474     248    1129

  Fragments arranged by size: 

                1129     474     248
 
ApoI r'AATT_y

Cuts at:      0     181     294     364     729     896    1472    1587    1851 
   Size:        181     113      70     365     167     576     115     264

  Fragments arranged by size: 

                 576     365     264     181     167     115     113      70
            
 
BamHI G'GATC_C

Cuts at:      0     675    1851 
   Size:        675    1176
 
BanII G_rGCy'C

Cuts at:      0     662    1851 
   Size:        662    1189
 
BbvCI CC'TCA_GC

Cuts at:      0     246     494    1157    1428    1851 
   Size:        246     248     663     271     423

  Fragments arranged by size: 

                 663     423     271     248     246
 
BclI T'GATC_A

Cuts at:      0     234    1851 
   Size:        234    1617
 
BfrBI ATG'CAT

Cuts at:      0     420    1851 
   Size:        420    1431
 
BglII A'GATC_T

Cuts at:      0     225    1851 
   Size:        225    1626
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     119    1851 
   Size:        119    1732
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     104     509    1851 
   Size:        104     405    1342

  Fragments arranged by size: 

                1342     405     104
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     349    1851 
   Size:        349    1502
 
Bpu10I CC'TnA_GC

Cuts at:      0     246     494    1157    1428    1851 
   Size:        246     248     663     271     423

  Fragments arranged by size: 

                 663     423     271     248     246
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     607    1851 
   Size:        607    1244
 
BsaBI GATnn'nnATC

Cuts at:      0     455    1851 
   Size:        455    1396
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1332    1362    1851 
   Size:       1332      30     489

  Fragments arranged by size: 

                1332     489      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     978    1513    1851 
   Size:        978     535     338

  Fragments arranged by size: 

                 978     535     338
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1440    1851 
   Size:       1440     411
 
BsmI GAATG_Cn'

Cuts at:      0     718     810    1736    1803    1851 
   Size:        718      92     926      67      48

  Fragments arranged by size: 

                 926     718      92      67      48
 
Bsp1286I G_dGCh'C

Cuts at:      0     662    1851 
   Size:        662    1189
 
BspHI T'CATG_A

Cuts at:      0    1077    1851 
   Size:       1077     774
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     310    1483    1851 
   Size:        310    1173     368

  Fragments arranged by size: 

                1173     368     310
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     256     722    1807    1851 
   Size:        256     466    1085      44

  Fragments arranged by size: 

                1085     466     256      44
 
BstYI r'GATC_y

Cuts at:      0     225     675     834    1851 
   Size:        225     450     159    1017

  Fragments arranged by size: 

                1017     450     225     159
 
Bsu36I CC'TnA_GG

Cuts at:      0    1461    1851 
   Size:       1461     390
 
BtsI GCAGTG_nn'

Cuts at:      0     723    1452    1851 
   Size:        723     729     399

  Fragments arranged by size: 

                 729     723     399
 
ClaI AT'CG_AT

Cuts at:      0     752    1851 
   Size:        752    1099
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1710    1745    1851 
   Size:       1710      35     106

  Fragments arranged by size: 

                1710     106      35
 
EaeI y'GGCC_r

Cuts at:      0     978    1851 
   Size:        978     873
 
EarI CTCTTCn'nnn_

Cuts at:      0    1305    1851 
   Size:       1305     546
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       2      32      89     158     349     617     692    1094 
   Size:          2      30      57      69     191     268      75     402

Cuts at:   1094    1145    1320    1851 
   Size:         51     175     531

  Fragments arranged by size: 

                 531     402     268     191     175      75      69      57
                  51      30       2
 
EcoO109I rG'GnC_Cy

Cuts at:      0     357     582    1851 
   Size:        357     225    1269

  Fragments arranged by size: 

                1269     357     225
 
EcoRI G'AATT_C

Cuts at:      0     181     294    1587    1851 
   Size:        181     113    1293     264

  Fragments arranged by size: 

                1293     264     181     113
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     229     583    1851 
   Size:        229     354    1268

  Fragments arranged by size: 

                1268     354     229
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1342    1374    1607    1639    1851 
   Size:       1342      32     233      32     212

  Fragments arranged by size: 

                1342     233     212      32      32
 
HincII GTy'rAC

Cuts at:      0    1361    1851 
   Size:       1361     490
 
HindIII A'AGCT_T

Cuts at:      0     545    1851 
   Size:        545    1306
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     286    1851 
   Size:        286    1565
 
MscI TGG'CCA

Cuts at:      0     980    1851 
   Size:        980     871
 
MslI CAynn'nnrTG

Cuts at:      0     876    1851 
   Size:        876     975
 
MspA1I CmG'CkG

Cuts at:      0     178    1851 
   Size:        178    1673
 
NsiI A_TGCA'T

Cuts at:      0     422    1851 
   Size:        422    1429
 
PasI CC'CwG_GG

Cuts at:      0     354    1851 
   Size:        354    1497
 
PpuMI rG'GwC_Cy

Cuts at:      0     582    1851 
   Size:        582    1269
 
PshAI GACnn'nnGTC

Cuts at:      0     272    1571    1851 
   Size:        272    1299     280

  Fragments arranged by size: 

                1299     280     272
 
PvuII CAG'CTG

Cuts at:      0     178    1851 
   Size:        178    1673
 
SfcI C'TryA_G

Cuts at:      0    1319    1851 
   Size:       1319     532
 
SmlI C'TyrA_G

Cuts at:      0     586    1851 
   Size:        586    1265
 
StuI AGG'CCT

Cuts at:      0    1605    1851 
   Size:       1605     246
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     367    1494    1851 
   Size:        367    1127     357

  Fragments arranged by size: 

                1127     367     357
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1332    1364    1851 
   Size:       1332      32     487

  Fragments arranged by size: 

                1332     487      32
 
Tth111I GACn'n_nGTC

Cuts at:      0      56    1851 
   Size:         56    1795
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1554    1851 
   Size:       1554     297
 

 Enzymes that do cut:

     AarI     AcuI    AlwNI     ApoI    BamHI    BanII    BbvCI     BclI
    BfrBI    BglII     BmrI     BplI     BpmI   Bpu10I    BpuEI    BsaBI
    BsaXI    BseRI     BsgI     BsmI Bsp1286I    BspHI    BspMI   BstAPI
    BstYI   Bsu36I     BtsI     ClaI    CspCI     EaeI     EarI  Eco57MI
 EcoO109I    EcoRI     FalI    Hin4I   HincII  HindIII     MmeI     MscI
     MslI   MspA1I     NsiI     PasI    PpuMI    PshAI    PvuII     SfcI
     SmlI     StuI    TaqII     TstI  Tth111I     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI     BanI     BbeI     BbsI     BcgI
    BciVI     BglI     BlpI Bme1580I    BmgBI     BmtI     BsaI    BsaAI
    BsaHI    BsaWI    BseYI    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII
    BstXI  BstZ17I     BtgI    BtgZI     DraI   DraIII     DrdI     EagI
     EciI  EcoICRI    EcoNI    EcoRV     FseI     FspI    FspAI    HaeII
     HpaI     KasI     KpnI     MfeI     MluI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NspI     PacI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StyI     SwaI
     TatI     XbaI     XhoI     XmaI     XmnI     ZraI
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