Restriction map for pF1KE0246
##################################
# NO CUT ENZYMES (  118 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AgeI        AlwI        AocI        
ApaI        ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspI        AviII       AvrII       
BamHI       BanII       BbsI        BbvCI       BciVI       
BclI        BfaI        BfrI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaBI       BsaI        
BsaWI       BseAI       BsiWI       BsmI        BspDI       
BspEI       BspHI       BspLU11I    BsrBI       BssHII      
BssSI       Bst1107I    BstBI       BstYI       BstZ17I     
Bsu36I      CelII       ClaI        CpoI        DpnII       
DraI        Eco81I      EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HindIII     HpaI        
KpnI        MaeI        MamI        MboI        MfeI        
MflI        MroI        MseI        MunI        NdeI        
NdeII       NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PflFI       PflMI       
PinAI       PmeI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        PvuII       RcaI        RsrII       
SacI        SalI        Sau3AI      SbfI        ScaI        
SexAI       SfaNI       SfuI        SnaBI       SpeI        
SphI        Sse8387I    SspI        StuI        SwaI        
TfiI        Tru9I       Tsp509I     Tth111I     Van91I      
XbaI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AflIII        122  ACGCGT               A^C[GA][CT]GT
AhdI         1198  GACAGCGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI       494  AGCGCT               AGC^GCT
AspEI        1198  GACAGCGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI          965  GGCGCC               GGCGC^C
BbrPI         958  CACGTG               CAC^GTG
BcgI          860  GCATGGCCATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BseRI          79  GAGGAG               GAGGAG(10/8)
BsmFI         540  GTCCC                GGGAC(10/14)
BspMI         335  ACCTGC               ACCTGC(4/8)
BsrDI        1229  GCAATG               GCAATG(2/0)
DdeI          484  CTGAG                C^T[ACGT]AG
DraII         887  AGGCCCT              [GA]G^G[ACGT]CC[CT]
DraIII        898  CACGGCGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          531  GACCCTCATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I     1198  GACAGCGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      494  AGCGCT               AGC^GCT
EcoNI        1181  CCTTCTCGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      887  AGGCCCT              [GA]G^G[ACGT]CC[CT]
HincII        715  GTCAAC               GT[CT]^[GA]AC
HindII        715  GTCAAC               GT[CT]^[GA]AC
HinfI        1002  GACTC                G^A[ACGT]TC
KasI          965  GGCGCC               G^GCGCC
KspI          423  CCGCGG               CCGC^GG
MluI          122  ACGCGT               A^CGCGT
NarI          965  GGCGCC               GG^CGCC
PaeR7I       1185  CTCGAG               C^TCGAG
PleI         1002  GACTC                GAGTC(4/5)
PmaCI         958  CACGTG               CAC^GTG
PmlI          958  CACGTG               CAC^GTG
SacII         423  CCGCGG               CCGC^GG
SapI          216  GCTCTTC              GCTCTTC(1/4)
SfiI         1128  GGCCACACTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI          965  GGCGCC               GGC^GCC
SgrAI         654  CGCCGGCG             C[GA]^CCGG[CT]G
SmlI         1185  CTCGAG               C^T[CT][GA]AG
XhoI         1185  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AlwNI         283  CAGAGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              886  CAGGCCCTG           
AvaII         759  GGTCC                G^G[AT]CC
              882  GGTCC               
BglI          589  GCCCTGCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1129  GCCACACTGGC         
BpmI         1224  CTCCAG               CTGGAG(16/14)
               76  CTGGAG              
BsaAI         914  TACGTG               [CT]AC^GT[GA]
              958  CACGTG              
BsgI          450  CTGCAC               GTGCAG(16/14)
              917  GTGCAG              
BsmAI        1243  GAGAC                GTCTC(1/5)
             1099  GTCTC               
BsmBI        1098  CGTCTC               CGTCTC(1/5)
             1243  GAGACG              
Bsp1407I      371  TGTACA               T^GTACA
             1088  TGTACA              
BsrGI         371  TGTACA               T^GTACA
             1088  TGTACA              
BsrI           75  ACTGG                ACTGG(1/-1)
             1134  ACTGG               
BstAPI        282  GCAGAGCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              897  GCACGGCGTGC         
BstEII        195  GGTGACC              G^GT[ACGT]ACC
             1041  GGTGACC             
BstPI         195  GGTGACC              G^GT[ACGT]ACC
             1041  GGTGACC             
BstXI         185  CCAAGGTGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              356  CCATGCCCGTGG        
EagI           41  CGGCCG               C^GGCCG
              809  CGGCCG              
EclXI          41  CGGCCG               C^GGCCG
              809  CGGCCG              
Eco52I         41  CGGCCG               C^GGCCG
              809  CGGCCG              
EcoO65I       195  GGTGACC              G^GT[ACGT]ACC
             1041  GGTGACC             
FauI         1238  CCCGC                CCCGC(4/6)
               60  GCGGG               
MspA1I         58  CAGCGG               C[AC]G^C[GT]G
              423  CCGCGG              
NcoI          617  CCATGG               C^CATGG
              743  CCATGG              
PstI          280  CTGCAG               CTGCA^G
              289  CTGCAG              
SfcI          280  CTGCAG               C^T[GA][CT]AG
              289  CTGCAG              
SmaI           30  CCCGGG               CCC^GGG
               96  CCCGGG              
SspBI         371  TGTACA               T^GTACA
             1088  TGTACA              
TaqI          869  TCGA                 T^CGA
             1186  TCGA                
TthHB8I       869  TCGA                 T^CGA
             1186  TCGA                
XcmI          149  CCAGCAGCGAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              479  CCAGCCTGAGTGTGG     
XmaCI          30  CCCGGG               C^CCGGG
               96  CCCGGG              
XmaI           30  CCCGGG               C^CCGGG
               96  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   72 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
Alw44I      ApaLI       AspHI       AvaI        BalI        
BanI        BbvI        BcnI        BmyI        BsaHI       
BsaJI       BsiEI       BsiHKAI     BsiYI       BslI        
BsoBI       Bsp1286I    BsrFI       BssKI       BstF5I      
BstNI       BstUI       Cac8I       CfoI        Cfr10I      
Cfr13I      DsaI        EaeI        EarI        EcoRII      
EcoT14I     Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HpaII       HphI        ItaI        Ksp632I     MaeII       
MaeIII      MboII       MluNI       MnlI        MscI        
MslI        MspI        MvaI        MvnI        MwoI        
NaeI        NciI        NgoMIV      NlaIII      NlaIV       
RsaI        Sau96I      ScrFI       StyI        TseI        
Tsp45I      TspRI       
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