Restriction map for
pF1KE0236
(Linear) (Six-base) MAPSORT of: KIEE0236.seq Check: 2342 from: 1 to: 828
KIEE0236.orf
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AclI AA'CG_TT
Cuts at: 0 391 828
Size: 391 437
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 492 828
Size: 492 336
ApaI G_GGCC'C
Cuts at: 0 565 828
Size: 565 263
ApoI r'AATT_y
Cuts at: 0 3 828
Size: 3 825
BanI G'GyrC_C
Cuts at: 0 412 828
Size: 412 416
BanII G_rGCy'C
Cuts at: 0 377 565 828
Size: 377 188 263
Fragments arranged by size:
377 263 188
BbsI GAAGACnn'nnnn_
Cuts at: 0 522 828
Size: 522 306
BbvCI CC'TCA_GC
Cuts at: 0 639 828
Size: 639 189
Bme1580I G_kGCm'C
Cuts at: 0 415 565 828
Size: 415 150 263
Fragments arranged by size:
415 263 150
BmrI ACTGGGnnnn_n'
Cuts at: 0 371 828
Size: 371 457
Bpu10I CC'TnA_GC
Cuts at: 0 639 828
Size: 639 189
BsaI GGTCTCn'nnnn_
Cuts at: 0 547 828
Size: 547 281
BsaHI Gr'CG_yC
Cuts at: 0 347 828
Size: 347 481
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 280 642 828
Size: 280 362 186
Fragments arranged by size:
362 280 186
BseYI C'CCAG_C
Cuts at: 0 575 828
Size: 575 253
BsmI GAATG_Cn'
Cuts at: 0 20 828
Size: 20 808
Bsp1286I G_dGCh'C
Cuts at: 0 377 415 565 828
Size: 377 38 150 263
Fragments arranged by size:
377 263 150 38
BspHI T'CATG_A
Cuts at: 0 308 753 828
Size: 308 445 75
Fragments arranged by size:
445 308 75
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 245 828
Size: 245 583
BstBI TT'CG_AA
Cuts at: 0 387 828
Size: 387 441
Bsu36I CC'TnA_GG
Cuts at: 0 274 828
Size: 274 554
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 456 828
Size: 456 372
DraI TTT'AAA
Cuts at: 0 405 828
Size: 405 423
DrdI GACnn_nn'nnGTC
Cuts at: 0 190 828
Size: 190 638
EaeI y'GGCC_r
Cuts at: 0 236 828
Size: 236 592
EarI CTCTTCn'nnn_
Cuts at: 0 389 448 511 828
Size: 389 59 63 317
Fragments arranged by size:
389 317 63 59
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 231 828
Size: 231 597
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 492 828
Size: 492 336
EcoO109I rG'GnC_Cy
Cuts at: 0 192 828
Size: 192 636
EcoRI G'AATT_C
Cuts at: 0 3 828
Size: 3 825
MslI CAynn'nnrTG
Cuts at: 0 340 828
Size: 340 488
NspI r_CATG'y
Cuts at: 0 451 828
Size: 451 377
PasI CC'CwG_GG
Cuts at: 0 558 828
Size: 558 270
PfoI T'CCnGG_A
Cuts at: 0 194 485 828
Size: 194 291 343
Fragments arranged by size:
343 291 194
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 80 112 828
Size: 80 32 716
Fragments arranged by size:
716 80 32
PpuMI rG'GwC_Cy
Cuts at: 0 192 828
Size: 192 636
PsiI TTA'TAA
Cuts at: 0 136 828
Size: 136 692
PspOMI G'GGCC_C
Cuts at: 0 561 828
Size: 561 267
SapI GCTCTTCn'nnn_
Cuts at: 0 448 828
Size: 448 380
SexAI A'CCwGG_T
Cuts at: 0 524 828
Size: 524 304
SspI AAT'ATT
Cuts at: 0 714 805 828
Size: 714 91 23
Fragments arranged by size:
714 91 23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 451 828
Size: 451 377
XmnI GAAnn'nnTTC
Cuts at: 0 89 541 828
Size: 89 452 287
Fragments arranged by size:
452 287 89
Enzymes that do cut:
AclI AcuI ApaI ApoI BanI BanII BbsI BbvCI
Bme1580I BmrI Bpu10I BsaI BsaHI BseRI BseYI BsmI
Bsp1286I BspHI BspMI BstBI Bsu36I BtgZI DraI DrdI
EaeI EarI EciI Eco57MI EcoO109I EcoRI MslI NspI
PasI PfoI PpiI PpuMI PsiI PspOMI SapI SexAI
SspI TaqII XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BcgI BciVI
BclI BfrBI BglI BglII BlpI BmgBI BmtI BplI
BpmI BpuEI BsaAI BsaBI BsaWI BsaXI BsgI BsiEI
BsiHKAI BsiWI BsmBI BspEI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstEII BstXI BstYI BstZ17I BtgI
BtsI ClaI CspCI DraIII EagI EcoICRI EcoNI EcoRV
FalI FseI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PciI PflMI PmeI PmlI PshAI PspXI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI StuI StyI
SwaI TatI TstI Tth111I XbaI XcmI XhoI XmaI
ZraI