Restriction map for pF1KE2019
##################################
# NO CUT ENZYMES (  123 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwNI       AocI        Aor51HI     ApaLI       AscI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AviII       AvrII       BamHI       BanI        BbeI        
BbrPI       BbvCI       BcgI        BciVI       BfrI        
BglI        BglII       BlnI        BsaAI       BsaBI       
BsaHI       BsaI        BseAI       BseRI       BsiHKAI     
BsiWI       BsmBI       BsmI        BspDI       BspEI       
BspLU11I    BsrDI       BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstZ17I     Bsu36I      
ClaI        CpoI        DraI        DraII       DrdI        
DsaI        Eam1105I    Eco47III    Eco81I      EcoO109I    
EcoO65I     EcoRI       EcoRV       FseI        FspI        
HgaI        Hin1I       HindIII     HpaI        KasI        
KpnI        KspI        MaeII       MamI        MfeI        
MluI        MroI        MunI        NaeI        NarI        
NcoI        NgoMIV      NheI        NotI        NruI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
Psp1406I    PstI        PvuI        RsrII       SacI        
SacII       SbfI        ScaI        SfcI        SfiI        
SfoI        SfuI        SgrAI       SnaBI       SpeI        
Sse8387I    SspI        StuI        SwaI        Tth111I     
XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

ApaI          118  GGGCCC               GGGCC^C
AseI          886  ATTAAT               AT^TAAT
AsnI          886  ATTAAT               AT^TAAT
AvaI          115  CCCGGG               C^[CT]CG[GA]G
AvaII         549  GGACC                G^G[AT]CC
BanII         118  GGGCCC               G[GA]GC[CT]^C
BbsI         1691  GTCTTC               GAAGAC(2/6)
BclI         1662  TGATCA               T^GATCA
BlpI         1366  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1366  GCTGAGC              GC^T[ACGT]AGC
BpuAI        1691  GTCTTC               GAAGAC(2/6)
BsaWI         551  ACCGGA               [AT]^CCGG[AT]
BsiEI         723  CGGCCG               CG[GA][CT]^CG
BsoBI         115  CCCGGG               C^[CT]CG[GA]G
Bsp1407I     1715  TGTACA               T^GTACA
BspHI        1665  TCATGA               T^CATGA
BsrBI         720  GAGCGG               CCGCTC(-3/-3)
BsrFI          29  GCCGGT               [GA]^CCGG[CT]
BsrGI        1715  TGTACA               T^GTACA
BstAPI        616  GCAGGTTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI        1008  CCATGTGGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         233  GGATCT               [GA]^GATC[CT]
CelII        1366  GCTGAGC              GC^T[ACGT]AGC
Cfr10I         29  GCCGGT               [GA]^CCGG[CT]
DraIII       1630  CACGCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI          723  CGGCCG               C^GGCCG
EclXI         723  CGGCCG               C^GGCCG
Eco52I        723  CGGCCG               C^GGCCG
FbaI         1662  TGATCA               T^GATCA
HaeII        1670  AGCGCC               [GA]GCGC^[CT]
MflI          233  GGATCT               [GA]^GATC[CT]
MspA1I        523  CAGCTG               C[AC]G^C[GT]G
NdeI          329  CATATG               CA^TATG
PflMI        1629  CCACGCTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI         886  ATTAAT               AT^TAAT
PvuII         523  CAGCTG               CAG^CTG
RcaI         1665  TCATGA               T^CATGA
SapI         1699  GAAGAGC              GCTCTTC(1/4)
SexAI         962  ACCTGGT              A^CC[AT]GGT
SmaI          115  CCCGGG               CCC^GGG
SmlI          810  CTTGAG               C^T[CT][GA]AG
SphI         1493  GCATGC               GCATG^C
SspBI        1715  TGTACA               T^GTACA
Van91I       1629  CCACGCTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         233  GGATCT               [GA]^GATC[CT]
XmaCI         115  CCCGGG               C^CCGGG
XmaI          115  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AccI          302  GTCGAC               GT^[AC][GT]AC
              855  GTCGAC              
AccII          56  CGCG                 CG^CG
               58  CGCG                
AcsI          406  AAATTT               [GA]^AATT[CT]
              475  GAATTT              
ApoI          406  AAATTT               [GA]^AATT[CT]
              475  GAATTT              
BalI          609  TGGCCA               TGG^CCA
             1034  TGGCCA              
BfaI          697  CTAG                 C^TAG
             1141  CTAG                
BmyI          118  GGGCCC               G[AGT]GC[ACT]^C
              728  GGGCAC              
BpmI          745  CTCCAG               CTGGAG(16/14)
              986  CTGGAG              
BsgI          560  GTGCAG               GTGCAG(16/14)
             1069  GTGCAG              
Bsp1286I      118  GGGCCC               G[AGT]GC[ACT]^C
              728  GGGCAC              
BspMI         568  ACCTGC               ACCTGC(4/8)
              616  GCAGGT              
BstNI         633  CCAGG                CC^[AT]GG
              963  CCTGG               
BstUI          56  CGCG                 CG^CG
               58  CGCG                
EarI         1075  GAAGAG               CTCTTC(1/4)
             1699  GAAGAG              
EcoNI         338  CCTTTTGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              893  CCTTCAAGAGG         
EcoRII        633  CCAGG                ^CC[AT]GG
              963  CCTGG               
FauI           54  CCCGC                CCCGC(4/6)
               59  GCGGG               
Ksp632I      1075  GAAGAG               CTCTTC(1/4)
             1699  GAAGAG              
MaeI          697  CTAG                 C^TAG
             1141  CTAG                
MluNI         609  TGGCCA               TGG^CCA
             1034  TGGCCA              
MscI          609  TGGCCA               TGG^CCA
             1034  TGGCCA              
MslI          760  CACCCCAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1009  CATGTGGATG          
MvaI          633  CCAGG                CC^[AT]GG
              963  CCTGG               
MvnI           56  CGCG                 CG^CG
               58  CGCG                
NspI          247  GCATGT               [GA]CATG^[CT]
             1493  GCATGC              
SalI          302  GTCGAC               G^TCGAC
              855  GTCGAC              
TfiI          134  GAATC                G^A[AT]TC
             1285  GAATC               
Tsp45I       1099  GTCAC                ^GT[GC]AC
             1534  GTGAC               
XcmI          349  CCAAAGGAGAACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              992  CCACTGCTTGTGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   59 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        BbvI        
BcnI        BsaJI       BsiYI       BslI        BsmAI       
BsmFI       BsrI        BssKI       BstF5I      Cac8I       
CfoI        Cfr13I      DdeI        DpnII       EaeI        
EcoT14I     EcoT22I     Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HincII      HindII      
HinfI       HpaII       HphI        ItaI        MaeIII      
MboI        MboII       MnlI        MseI        MspI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NsiI        PleI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       StyI        TaqI        Tru9I       
TseI        Tsp509I     TspRI       TthHB8I     
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