Restriction map for pF1KE2019
 (Linear) (Six-base) MAPSORT of: KIEE2019.seq  Check: 5528  from: 1  to: 1788



KIEE2019.orf

 With 182 enzymes: SgfI * 

                        July 16, 2019 14:21  ..

AccI GT'mk_AC

Cuts at:      0     303     856    1788 
   Size:        303     553     932

  Fragments arranged by size: 

                 932     553     303
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1718    1788 
   Size:       1718      70
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     270     541     585    1075    1788 
   Size:        270     271      44     490     713

  Fragments arranged by size: 

                 713     490     271     270      44
 
ApaI G_GGCC'C

Cuts at:      0     122    1788 
   Size:        122    1666
 
ApoI r'AATT_y

Cuts at:      0     406     475    1788 
   Size:        406      69    1313

  Fragments arranged by size: 

                1313     406      69
 
AseI AT'TA_AT

Cuts at:      0     887    1788 
   Size:        887     901
 
AvaI C'yCGr_G

Cuts at:      0     115    1788 
   Size:        115    1673
 
BanII G_rGCy'C

Cuts at:      0     122    1788 
   Size:        122    1666
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1684    1788 
   Size:       1684     104
 
BclI T'GATC_A

Cuts at:      0    1662    1788 
   Size:       1662     126
 
BfrBI ATG'CAT

Cuts at:      0    1449    1497    1551    1788 
   Size:       1449      48      54     237

  Fragments arranged by size: 

                1449     237      54      48
 
BlpI GC'TnA_GC

Cuts at:      0    1367    1788 
   Size:       1367     421
 
Bme1580I G_kGCm'C

Cuts at:      0     122     732    1788 
   Size:        122     610    1056

  Fragments arranged by size: 

                1056     610     122
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     133    1788 
   Size:        133    1655
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1101    1692    1788 
   Size:       1101     591      96

  Fragments arranged by size: 

                1101     591      96
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     730    1007    1788 
   Size:        730     277     781

  Fragments arranged by size: 

                 781     730     277
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     831    1788 
   Size:        831     957
 
BsaWI w'CCGG_w

Cuts at:      0     551    1788 
   Size:        551    1237
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      90     120     346     376     728     758    1788 
   Size:         90      30     226      30     352      30    1030

  Fragments arranged by size: 

                1030     352     226      90      30      30      30
 
BseYI C'CCAG_C

Cuts at:      0    1062    1788 
   Size:       1062     726
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     581    1090    1788 
   Size:        581     509     698

  Fragments arranged by size: 

                 698     581     509
 
BsiEI CG_ry'CG

Cuts at:      0     726    1788 
   Size:        726    1062
 
Bsp1286I G_dGCh'C

Cuts at:      0     122     732    1788 
   Size:        122     610    1056

  Fragments arranged by size: 

                1056     610     122
 
BspHI T'CATG_A

Cuts at:      0    1665    1788 
   Size:       1665     123
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     577     607    1788 
   Size:        577      30    1181

  Fragments arranged by size: 

                1181     577      30
 
BsrBI CCG'CTC

Cuts at:      0     722    1788 
   Size:        722    1066
 
BsrFI r'CCGG_y

Cuts at:      0      29    1788 
   Size:         29    1759
 
BsrGI T'GTAC_A

Cuts at:      0    1715    1788 
   Size:       1715      73
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     622    1788 
   Size:        622    1166
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1015    1788 
   Size:       1015     773
 
BstYI r'GATC_y

Cuts at:      0     233    1788 
   Size:        233    1555
 
BtsI GCAGTG_nn'

Cuts at:      0     242     719     730     992    1361    1788 
   Size:        242     477      11     262     369     427

  Fragments arranged by size: 

                 477     427     369     262     242      11
 
DraIII CAC_nnn'GTG

Cuts at:      0    1635    1788 
   Size:       1635     153
 
EaeI y'GGCC_r

Cuts at:      0     609     723    1034    1788 
   Size:        609     114     311     754

  Fragments arranged by size: 

                 754     609     311     114
 
EagI C'GGCC_G

Cuts at:      0     723    1788 
   Size:        723    1065
 
EarI CTCTTCn'nnn_

Cuts at:      0    1070    1694    1788 
   Size:       1070     624      94

  Fragments arranged by size: 

                1070     624      94
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     730    1007    1718    1788 
   Size:        730     277     711      70

  Fragments arranged by size: 

                 730     711     277      70
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     342     897    1788 
   Size:        342     555     891

  Fragments arranged by size: 

                 891     555     342
 
HaeII r_GCGC'y

Cuts at:      0    1674    1788 
   Size:       1674     114
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1647    1679    1788 
   Size:       1647      32     109

  Fragments arranged by size: 

                1647     109      32
 
HincII GTy'rAC

Cuts at:      0     304     857    1023    1788 
   Size:        304     553     166     765

  Fragments arranged by size: 

                 765     553     304     166
 
MscI TGG'CCA

Cuts at:      0     611    1036    1788 
   Size:        611     425     752

  Fragments arranged by size: 

                 752     611     425
 
MslI CAynn'nnrTG

Cuts at:      0     764    1013    1788 
   Size:        764     249     775

  Fragments arranged by size: 

                 775     764     249
 
MspA1I CmG'CkG

Cuts at:      0     525    1788 
   Size:        525    1263
 
NdeI CA'TA_TG

Cuts at:      0     330    1788 
   Size:        330    1458
 
NsiI A_TGCA'T

Cuts at:      0    1451    1499    1553    1788 
   Size:       1451      48      54     235

  Fragments arranged by size: 

                1451     235      54      48
 
NspI r_CATG'y

Cuts at:      0     251    1497    1788 
   Size:        251    1246     291

  Fragments arranged by size: 

                1246     291     251
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1635    1788 
   Size:       1635     153
 
PspOMI G'GGCC_C

Cuts at:      0     118    1788 
   Size:        118    1670
 
PvuII CAG'CTG

Cuts at:      0     525    1788 
   Size:        525    1263
 
SalI G'TCGA_C

Cuts at:      0     302     855    1788 
   Size:        302     553     933

  Fragments arranged by size: 

                 933     553     302
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1694    1788 
   Size:       1694      94
 
SexAI A'CCwGG_T

Cuts at:      0     962    1788 
   Size:        962     826
 
SmaI CCC'GGG

Cuts at:      0     117    1788 
   Size:        117    1671
 
SmlI C'TyrA_G

Cuts at:      0     810    1788 
   Size:        810     978
 
SphI G_CATG'C

Cuts at:      0    1497    1788 
   Size:       1497     291
 
StyI C'CwwG_G

Cuts at:      0     375     504     920    1788 
   Size:        375     129     416     868

  Fragments arranged by size: 

                 868     416     375     129
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     388     555    1054    1465    1788 
   Size:        388     167     499     411     323

  Fragments arranged by size: 

                 499     411     388     323     167
 
TatI w'GTAC_w

Cuts at:      0     368     797    1715    1788 
   Size:        368     429     918      73

  Fragments arranged by size: 

                 918     429     368      73
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      90     122     306     338     346     378    1461    1493 
   Size:         90      32     184      32       8      32    1083      32

Cuts at:   1493    1788 
   Size:        295

  Fragments arranged by size: 

                1083     295     184      90      32      32      32      32
                   8
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     356     999    1788 
   Size:        356     643     789

  Fragments arranged by size: 

                 789     643     356
 
XmaI C'CCGG_G

Cuts at:      0     115    1788 
   Size:        115    1673
 

 Enzymes that do cut:

     AccI     AcuI     AlfI     ApaI     ApoI     AseI     AvaI    BanII
     BbsI     BclI    BfrBI     BlpI Bme1580I     BmrI     BplI     BpmI
    BpuEI    BsaWI    BsaXI    BseYI     BsgI    BsiEI Bsp1286I    BspHI
    BspMI    BsrBI    BsrFI    BsrGI   BstAPI    BstXI    BstYI     BtsI
   DraIII     EaeI     EagI     EarI  Eco57MI    EcoNI    HaeII    Hin4I
   HincII     MscI     MslI   MspA1I     NdeI     NsiI     NspI    PflMI
   PspOMI    PvuII     SalI     SapI    SexAI     SmaI     SmlI     SphI
     StyI    TaqII     TatI     TstI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI    AlwNI    ApaLI     AscI    AsiSI    AvrII
     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI    BciVI     BglI
    BglII    BmgBI     BmtI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI
    BseRI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BsrDI   BssHII
    BssSI    BstBI   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI
    CspCI     DraI     DrdI     EciI  EcoICRI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     NaeI     NarI     NcoI   NgoMIV     NheI
     NotI     NruI     PacI     PasI     PciI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI     PstI     PvuI
    RsrII     SacI    SacII    SanDI     SbfI     ScaI     SfcI     SfiI
     SfoI     SgfI    SgrAI    SnaBI     SpeI     SrfI     SspI     StuI
     SwaI  Tth111I     XbaI     XhoI     XmnI     ZraI
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